OATP1 Antibody
(Synonyms: OATP, OATP1, OATP1A2, SLC21A3, SLCO1A2, Solute carrier organic anion transporter family member 1A2, OATP-A, Organic anion-transporting polypeptide 1, Sodium-independent organic anion transporter, Solute carrier family 21 member 3, OATP-1)OATP1 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to OATP1.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:100-200 |
Product Details
OATP1 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to OATP1.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 95 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 74 kDa
Synthetic peptide corresponding to the center region of human OATP1.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
OATP1 is a Na(+)-independent transporter that mediates the cellular uptake of a broad range of organic anions such as the endogenous bile salts cholate and deoxycholate, either in their unconjugated or conjugated forms (taurocholate and glycocholate), at the plasmam membrane. Responsible for intestinal absorption of bile acids (By similarity). Transports dehydroepiandrosterone 3-sulfate (DHEAS), a major circulating steroid secreted by the adrenal cortex, as well as estrone 3-sulfate and 17beta-estradiol 17-O-(beta-D-glucuronate). Mediates apical uptake of all-trans-retinol (atROL) across human retinal pigment epithelium, which is essential to maintaining the integrity of the visual cycle and thus vision. Involved in the uptake of clinically used drugs. Capable of thyroid hormone transport (both T3 or 3,3',5'-triiodo-L-thyronine, and T4 or L-tyroxine). Also transports prostaglandin E2. Plays roles in blood-brain and -cerebrospinal fluid barrier transport of organic anions and signal mediators, and in hormone uptake by neural cells (By similarity). May also play a role in the reuptake of neuropeptides such as substance P/TAC1 and vasoactive intestinal peptide/VIP released from retinal neurons. May play an important role in plasma and tissue distribution of the structurally diverse chemotherapeutic drugs methotrexate and paclitaxel. Shows a pH-sensitive substrate specificity which may be ascribed to the protonation state of the binding site and leads to a stimulation of substrate transport in an acidic microenvironment. Hydrogencarbonate/HCO3(-) acts as the probable counteranion that exchanges for organic anions. May contribute to regulate the transport of organic compounds in testis across the blood-testis-barrier (Probable)[1][2][3][4][5][6][7][8][9][10][11][12][13].
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Subcellular Localization
Cell membrane; Basal cell membrane
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Expression
Tissue_Specificity: Higher expression in the brain than in liver and kidney. Expressed in brain neurons in both cortex and hippocampus. Expressed in placental trophoblasts. Also expressed in lung and testes at lower levels. Expressed in the eye (at protein level). Expressed in the retina in the outer and inner nuclear layers, the inner plexiform layer and the ganglion cell layer. Expressed in liver and prostate. In testis, primarily localized to the basal membrane of Sertoli cells and weakly expressed in Leydig cells and within the tubules. Expressed in fetal brain and liver. -
Isoforms & Post-Translational Modification
OATP1 has 2 isoforms, P46721-1: amino acid length is 670, molecular weight is 74145 Da (predicted); P46721-2: amino acid length is 579, molecular weight is 64369 Da (predicted).
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SwissProt ID
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Synonyms
OATP, OATP1, OATP1A2, SLC21A3, SLCO1A2, Solute carrier organic anion transporter family member 1A2, OATP-A, Organic anion-transporting polypeptide 1, Sodium-independent organic anion transporter, Solute carrier family 21 member 3, OATP-1
Documentation
[1]. Leuthold S, et al. Mechanisms of pH-gradient driven transport mediated by organic anion polypeptide transporters. Am J Physiol Cell Physiol. 2009 Mar;296(3):C570-82. [Content Brief]
[2]. Kullak-Ublick GA, et al. Molecular and functional characterization of an organic anion transporting polypeptide cloned from human liver. Gastroenterology. 1995 Oct;109(4):1274-82. [Content Brief]
[3]. Kullak-Ublick GA, et al. Organic anion-transporting polypeptide B (OATP-B) and its functional comparison with three other OATPs of human liver. Gastroenterology. 2001 Feb;120(2):525-33. [Content Brief]
[4]. Briz O, et al. Role of organic anion-transporting polypeptides, OATP-A, OATP-C and OATP-8, in the human placenta-maternal liver tandem excretory pathway for foetal bilirubin. Biochem J. 2003 May 1;371(Pt 3):897-905. [Content Brief]
[5]. Zhou F, et al. Functional analysis of novel polymorphisms in the human SLCO1A2 gene that encodes the transporter OATP1A2. AAPS J. 2013 Oct;15(4):1099-108. [Content Brief]
[6]. Chan T, et al. Human organic anion transporting polypeptide 1A2 (OATP1A2) mediates cellular uptake of all-trans-retinol in human retinal pigmented epithelial cells. Br J Pharmacol. 2015 May;172(9):2343-53. [Content Brief]
[7]. Kullak-Ublick GA, et al. Dehydroepiandrosterone sulfate (DHEAS): identification of a carrier protein in human liver and brain. FEBS Lett. 1998 Mar 13;424(3):173-6. [Content Brief]
[8]. Bailey DG, et al. Naringin is a major and selective clinical inhibitor of organic anion-transporting polypeptide 1A2 (OATP1A2) in grapefruit juice. Clin Pharmacol Ther. 2007 Apr;81(4):495-502. [Content Brief]
[9]. Shirasaka Y, et al. Intestinal absorption of HMG-CoA reductase inhibitor pravastatin mediated by organic anion transporting polypeptide. Pharm Res. 2010 Oct;27(10):2141-9. [Content Brief]
[10]. Lee HH, et al. Contribution of Organic Anion-Transporting Polypeptides 1A/1B to Doxorubicin Uptake and Clearance. Mol Pharmacol. 2017 Jan;91(1):14-24. [Content Brief]
[11]. Ianculescu AG, et al. Transport of thyroid hormones is selectively inhibited by 3-iodothyronamine. Mol Biosyst. 2010 Aug;6(8):1403-10. [Content Brief]
[12]. Gao B, et al. Differential cellular expression of organic anion transporting peptides OATP1A2 and OATP2B1 in the human retina and brain: implications for carrier-mediated transport of neuropeptides and neurosteriods in the CNS. Pflugers Arch. 2015 Jul;467(7):1481-1493. [Content Brief]
[13]. van de Steeg E, et al. Influence of human OATP1B1, OATP1B3, and OATP1A2 on the pharmacokinetics of methotrexate and paclitaxel in humanized transgenic mice. Clin Cancer Res. 2013 Feb 15;19(4):821-32. [Content Brief]
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