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DYKDDDDK Tag (FLAG) Antibody (YA780)

(Synonyms: FLAG Tag; flag)
3 Cited Publications
Customer Review

Based on 3 publication(s) in Google Scholar

DYKDDDDK Tag (FLAG) Antibody (YA780) is a non-conjugated and Mouse origined IgG1 monoclonal antibody, targeting to DYKDDDDK Tag(FLAG).

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, ICC/IF, IP

  • Reactivity :

    Species independent

  • Formulation:

    Supplied in 1*PBS (pH7.4), 0.2% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for DYKDDDDK Tag (FLAG) Antibody (YA780)
  • Experimental Validation Results for DYKDDDDK Tag (FLAG) Antibody (YA780)
  • DYKDDDDK Tag (FLAG) Antibody (YA780) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Publications Citing Use of MedChemExpress (MCE) DYKDDDDK Tag (FLAG) Antibody (YA780)

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3 Publications Citing Use of MCE DYKDDDDK Tag (FLAG) Antibody (YA780) (1)
IP
  • IP

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:5000-1:10000 1:100 2-5 µg/mL.

Product Details

Description

DYKDDDDK Tag (FLAG) Antibody (YA780) is a non-conjugated and Mouse origined IgG1 monoclonal antibody, targeting to DYKDDDDK Tag(FLAG).

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Species independent
  • Observed Molecular Weight
    Observed band size: 35 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 35 kDa
Immunogen

Synthetic peptide corresponding to FLAG-tag.

Sensitivity

Endogenous

Purification

Protein G affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

RRID

AB_3102307

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 1*PBS (pH7.4), 0.2% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for DYKDDDDK Tag (FLAG) Antibody (YA780)
    Western blot analysis of protein Human SIRT5(lane 2(10ng) and lane 3(20ng)) and Human BRD4(lane 4(10ng) and lane 5(20ng)) using Flag Tag (HY-P80111) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    DYKDDDDK Tag is a FLAG Tag is a multifunctional fusion tag for the purification of recombinant proteins. FLAG Tag maintances the natural folding of its fusing proteins. FLAG Tag can be removed by enterokinase, and eluted under non-denaturing conditions.

  • Synonyms

    FLAG Tag; flag

  • Research Field

    Tags & Cell Markers

DYKDDDDK Tag (FLAG) Antibody (YA780) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of protein Human SIRT5(lane 2(10ng) and lane 3(20ng)) and Human BRD4(lane 4(10ng) and lane 5(20ng)) using Flag Tag (HY-P80111) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    DYKDDDDK Tag (FLAG) Antibody (YA780) purchased from MedChemExpress. Usage Cited in: ACS Chem Biol. 2025 Jul 18;20(7):1646-1659. [Abstract]

    Customer Validation
     Targeted O-GlcNAcylation of CK2α by OGTAC-1 (100 nM) for 8 h significantly increased CK2α-CRBN interaction. Flag-CRBN, HTN-CK2α (WT or S347A), and fOGT were overexpressed in cells. HaloTrap was used for IP, and anti-FlagTag was used for co-IP detection.

  • DYKDDDDK Tag (FLAG) Antibody (YA780) Product Image
    1/16
  • Experimental Validation Results for DYKDDDDK Tag (FLAG) Antibody (YA780)
  • Experimental Validation Results for DYKDDDDK Tag (FLAG) Antibody (YA780)
  • DYKDDDDK Tag (FLAG) Antibody (YA780) Product Image
  • Experimental Validation Results for DYKDDDDK Tag (FLAG) Antibody (YA780)
    1/1
    MedchemExpress Validation
     Western blot analysis of protein Human SIRT5(lane 2(10ng) and lane 3(20ng)) and Human BRD4(lane 4(10ng) and lane 5(20ng)) using Flag Tag (HY-P80111) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for DYKDDDDK Tag (FLAG) Antibody (YA780)

  • DYKDDDDK Tag (FLAG) Antibody (YA780) purchased from MedChemExpress. Usage Cited in: ACS Chem Biol. 2025 Jul 18;20(7):1646-1659. [Abstract]

    Targeted O-GlcNAcylation of CK2α by OGTAC-1 (100 nM) for 8 h significantly increased CK2α-CRBN interaction. Flag-CRBN, HTN-CK2α (WT or S347A), and fOGT were overexpressed in cells. HaloTrap was used for IP, and anti-FlagTag was used for co-IP detection.

  • DYKDDDDK Tag (FLAG) Antibody (YA780) Product Image
    MedchemExpress Validation

    Western blot analysis of protein Human SIRT5(lane 2(10ng) and lane 3(20ng)) and Human BRD4(lane 4(10ng) and lane 5(20ng)) using Flag Tag (HY-P80111) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • DYKDDDDK Tag (FLAG) Antibody (YA780) Product Image WB