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  4. Monoclonal Antibodies Tag Antibodies
  5. DYKDDDDK Tag (FLAG) Antibody (YA780)

DYKDDDDK Tag (FLAG) Antibody (YA780)

Cat. No.: HY-P80111
COA
SDS
User Guide for Antibodies Technical Support

DYKDDDDK Tag (FLAG) Antibody (YA780) is a non-conjugated and Mouse origined IgG1 monoclonal antibody, targeting to DYKDDDDK Tag(FLAG).

For research use only. We do not sell to patients.

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Top Publications Citing Use of Products

3 Publications Citing Use of MCE DYKDDDDK Tag (FLAG) Antibody (YA780)

IP

    DYKDDDDK Tag (FLAG) Antibody (YA780) purchased from MCE. Usage Cited in: ACS Chem Biol. 2025 Jul 18;20(7):1646-1659.  [Abstract]

    Targeted O-GlcNAcylation of CK2α by OGTAC-1 (100 nM) for 8 h significantly increased CK2α-CRBN interaction. Flag-CRBN, HTN-CK2α (WT or S347A), and fOGT were overexpressed in cells. HaloTrap was used for IP, and anti-FlagTag was used for co-IP detection.
    • WB: Western Blot;
    • IHC-P: Immunohistochemistry-Paraffin;
    • IHC-F: Immunohistochemistry-Frozen;
    • ICC/IF: Immunocytochemistry/Immunofluorescence;
    • IF-Tissue: Immunofluorescence-Tissue;
    • mIHC: Multiplex Immunohistochemical;
    • IP: Immunoprecipitation;
    • ChIP: Chromatin Immunoprecipitation;
    • FC: Flow Cytometry;
    • ELISA: Enzyme Linked Immunosorbent Assay

    • Product Detail

    • Verification Image

    • Background

    • Documentation

    Description

    DYKDDDDK Tag (FLAG) Antibody (YA780) is a non-conjugated and Mouse origined IgG1 monoclonal antibody, targeting to DYKDDDDK Tag(FLAG).
    Host

    Mouse
    Clonality

    Monoclonal
    Molecular Weight
    Predicted band size: 35 kDa;
    Observed band size: 35 kDa
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
    Species Reactivity
    Species independent
    Immunogen

    Synthetic peptide corresponding to FLAG-tag.
    Application &
    Dilution Ratio
    Application Dilution Ratio
    WB
    WB: Western Blot
    		1:5000-1:10000
    ICC/IF
    ICC/IF: Immunocytochemistry/Immunofluorescence
    		1:100
    IP
    IP: Immunoprecipitation
    		2-5 µg/mL.
    Sensitivity Endogenous Purity Protein G affinity purified.
    Conjugation Non-conjugated Modification Unmodified
    Isotype IgG1  
    Appearance

    Liquid
    Formulation

    Supplied in 1*PBS (pH7.4), 0.2% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.
    Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
    Shipping

    Shipping with blue ice.
    Verification Image
    WB

    • Western blot analysis of protein Human SIRT5(lane 2(10ng) and lane 3(20ng)) and Human BRD4(lane 4(10ng) and lane 5(20ng)) using Flag Tag (HY-P80111) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

    Background
    Function：FLAG Tag is a multifunctional fusion tag for the purification of recombinant proteins. FLAG Tag maintances the natural folding of its fusing proteins. FLAG Tag can be removed by enterokinase, and eluted under non-denaturing conditions.
    RRID
    Research Field

    Tags & Cell Markers
    Synonyms
    FLAG Tag; flag
    Documentation
    SDS (251 KB)

    COA (206 KB)

    User Guide for Antibodies (1077 KB)

    DYKDDDDK Tag (FLAG) Antibody (YA780) Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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