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His Tag Antibody (YA4624)

(Synonyms: 6 His tag; HHHHHH tag)
1 Cited Publications
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Based on 1 publication(s) in Google Scholar

His Tag Antibody (YA4624) is a mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to His Tag.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, IP, IHC-P, ICC/IF, FC, ELISA

  • Reactivity :

    Species independent

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for His Tag Antibody (YA4624)
  • His Tag Antibody (YA4624) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Publications Citing Use of MedChemExpress (MCE) His Tag Antibody (YA4624)

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1 Publications Citing Use of MCE His Tag Antibody (YA4624) (1)
WB
  • WB

Applications

Application
WB Info
WB: Western Blot
IP Info
IP: Immunoprecipitation
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:1000 1:20 1:100-1:1000 1:100-1:1000 1:200-1:400 1:10000

Product Details

Description

His Tag Antibody (YA4624) is a mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to His Tag.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Species independent
  • Calculated Molecular Weight Predicted band size: 1 kDa
Immunogen

Synthesized peptide of His Tag (AA: HHHHHHHH-KLH).

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

RRID

AB_3719003

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Background

  • Function

    His Tag is a His tags are short peptide sequences (usually composed of 6-10 consecutive histidine residues) fused to the N-terminus or C-terminus of a target protein through genetic engineering. They are used for the efficient and rapid purification of recombinant proteins using metal chelate affinity chromatography.

  • Synonyms

    6 His tag; HHHHHH tag

His Tag Antibody (YA4624) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    His Tag Antibody (YA4624) purchased from MedChemExpress. Usage Cited in: SSRN. 2025 Nov 8.

    Customer Validation
     WB analysis with Flag beads for IP and anti-His antibody (1:1000, His Tag Antibody (YA4624), HY-P84927) in HEK293T cells transfected with empty His-tag vector (Vector), His-S6K2, or its mutants (S6K2-R283L, S6K2-E163A).

  • His Tag Antibody (YA4624) Product Image
    1/16
  • Experimental Validation Results for His Tag Antibody (YA4624)
  • His Tag Antibody (YA4624) Product Image

  • His Tag Antibody (YA4624) purchased from MedChemExpress. Usage Cited in: SSRN. 2025 Nov 8.

    WB analysis with Flag beads for IP and anti-His antibody (1:1000, His Tag Antibody (YA4624), HY-P84927) in HEK293T cells transfected with empty His-tag vector (Vector), His-S6K2, or its mutants (S6K2-R283L, S6K2-E163A).