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GFP Tag Antibody (YA415)

1 Cited Publications
Customer Review

Based on 1 publication(s) in Google Scholar

GFP Antibody is a non-conjugated and Rabbit origined IgG monoclonal antibody, targeting to GFP.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, IP, IF-Tissue

  • Reactivity :

    Species independent

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for GFP Tag Antibody (YA415)
  • Experimental Validation Results for GFP Tag Antibody (YA415)
  • Experimental Validation Results for GFP Tag Antibody (YA415)
  • Experimental Validation Results for GFP Tag Antibody (YA415)
  • GFP Tag Antibody (YA415) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Top Publications Citing

Use of MCE Products

Publications Citing Use of MedChemExpress (MCE) GFP Tag Antibody (YA415)

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1 Publications Citing Use of MCE GFP Tag Antibody (YA415) (3)
IPWB
  • IP
  • WB
  • WB

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IF-Tissue Info
IF-Tissue: Immunofluorescence-Tissue
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:5000-1:10000 1:200-1:500 1:100-1:500 1:100-1:500 2-5 µg/mL

Product Details

Description

GFP Antibody is a non-conjugated and Rabbit origined IgG monoclonal antibody, targeting to GFP.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Species independent
  • Observed Molecular Weight
    Observed band size: 27 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 27 kDa
Immunogen

Synthetic peptide corresponding to Aequorea victoria GFP.AA range:1-50.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102008

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for GFP Tag Antibody (YA415)
    Western blot analysis of extracts from GFP(lane 2(25ng) , GFP(lane 3(50ng) and GFP(lane 4(100ng)using GFP Antibody (HY-P80141) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin

  • Expression


    Tissue_specificity:Photocell

  • Subunit

    Monomer

  • SwissProt ID

    P42212

  • Research Field

    Tags & Cell Markers

GFP Tag Antibody (YA415) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from GFP(lane 2(25ng) , GFP(lane 3(50ng) and GFP(lane 4(100ng)using GFP Antibody (HY-P80141) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    GFP Tag Antibody (YA415) purchased from MedChemExpress. Usage Cited in: Signal Transduct Target Ther. 2025 Nov 18;10(1):373. [Abstract]

    Customer Validation
     293T cells were cotransfected with the FAM114A1-Myc plasmid together with the indicated E2F4-GFP (GFP Antibody; 5 µg; dilution 1:1000) mutants. The cells were collected for co-IP after 24 h. The samples were subjected to western blotting to determine interactions.

  • 1/16

    GFP Tag Antibody (YA415) purchased from MedChemExpress. Usage Cited in: Signal Transduct Target Ther. 2025 Nov 18;10(1):373. [Abstract]

    Customer Validation
     GFP Antibody (GFP) (dilution 1:1000). Py8119 cells transfected with indicated E2F4 mutants were collected for western blotting. Vinculin served as internal control.

  • 1/16

    GFP Tag Antibody (YA415) purchased from MedChemExpress. Usage Cited in: Signal Transduct Target Ther. 2025 Nov 18;10(1):373. [Abstract]

    Customer Validation
     Cytosol and nuclear fractions of the Py8119 cells were extracted and subjected to western blotting analysis. The ratio of nuclear E2F4-GFP (GFP Antibody; dilution 1:1000) was quantified. Vinculin and Lamin B1 served as internal controls for cytosol and nuclear fractions respectively.

  • GFP Tag Antibody (YA415) Product Image
    1/16
  • Experimental Validation Results for GFP Tag Antibody (YA415)
  • Experimental Validation Results for GFP Tag Antibody (YA415)
  • Experimental Validation Results for GFP Tag Antibody (YA415)
  • Experimental Validation Results for GFP Tag Antibody (YA415)
  • GFP Tag Antibody (YA415) Product Image
  • Experimental Validation Results for GFP Tag Antibody (YA415)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from GFP(lane 2(25ng) , GFP(lane 3(50ng) and GFP(lane 4(100ng)using GFP Antibody (HY-P80141) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for GFP Tag Antibody (YA415)

  • GFP Tag Antibody (YA415) purchased from MedChemExpress. Usage Cited in: Signal Transduct Target Ther. 2025 Nov 18;10(1):373. [Abstract]

    293T cells were cotransfected with the FAM114A1-Myc plasmid together with the indicated E2F4-GFP (GFP Antibody; 5 µg; dilution 1:1000) mutants. The cells were collected for co-IP after 24 h. The samples were subjected to western blotting to determine interactions.

  • GFP Tag Antibody (YA415) purchased from MedChemExpress. Usage Cited in: Signal Transduct Target Ther. 2025 Nov 18;10(1):373. [Abstract]

    GFP Antibody (GFP) (dilution 1:1000). Py8119 cells transfected with indicated E2F4 mutants were collected for western blotting. Vinculin served as internal control.

  • GFP Tag Antibody (YA415) purchased from MedChemExpress. Usage Cited in: Signal Transduct Target Ther. 2025 Nov 18;10(1):373. [Abstract]

    Cytosol and nuclear fractions of the Py8119 cells were extracted and subjected to western blotting analysis. The ratio of nuclear E2F4-GFP (GFP Antibody; dilution 1:1000) was quantified. Vinculin and Lamin B1 served as internal controls for cytosol and nuclear fractions respectively.

  • GFP Tag Antibody (YA415) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from GFP(lane 2(25ng) , GFP(lane 3(50ng) and GFP(lane 4(100ng)using GFP Antibody (HY-P80141) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • GFP Tag Antibody (YA415) Product Image WB