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  3. Primary Antibodies
  4. Monoclonal Antibodies Recombinant Antibodies Tag Antibodies
  5. GFP Tag Antibody (YA415)

GFP Antibody is a non-conjugated and Rabbit origined IgG monoclonal antibody, targeting to GFP.

For research use only. We do not sell to patients.

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Top Publications Citing Use of Products

1 Publications Citing Use of MCE GFP Tag Antibody (YA415)

  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

  • Verification Image

  • Background

  • Documentation

Description

GFP Antibody is a non-conjugated and Rabbit origined IgG monoclonal antibody, targeting to GFP.
Host

Rabbit
Clonality

Recombinant,Monoclonal
Molecular Weight
Predicted band size: 27 kDa;
Observed band size: 27 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Species independent
SwissProt ID
Immunogen

Synthetic peptide corresponding to Aequorea victoria GFP.AA range:1-50.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:5000-1:10000
IHC-P
IHC-P: Immunohistochemistry-Paraffin
1:200-1:500
ICC/IF
ICC/IF: Immunocytochemistry/Immunofluorescence
1:100-1:500
IF-Tissue
IF-Tissue: Immunofluorescence-Tissue
1:100-1:500
IP
IP: Immunoprecipitation
2-5 µg/mL
Sensitivity Endogenous Purity Protein A affinity purified.
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Verification Image
WB

  • Western blot analysis of extracts from GFP(lane 2(25ng) , GFP(lane 3(50ng) and GFP(lane 4(100ng)using GFP Antibody (HY-P80141) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background
Function：Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin
Expression：
Tissue_specificity:Photocell
Subunit：Monomer
RRID
Research Field

Tags & Cell Markers
Documentation
SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

GFP Tag Antibody (YA415) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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GFP Tag Antibody (YA415)
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HY-P80141
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