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c-Myc Antibody (YA497)

(Synonyms: MYC; BHLHE39; Myc proto-oncogene protein; Class E basic helix-loop-helix protein 39; bHLHe39; Proto-oncogene c-Myc; Transcription factor p64)
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Based on 7 publication(s) in Google Scholar

c-Myc Antibody (YA497) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to c-Myc.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for c-Myc Antibody (YA497)
  • Experimental Validation Results for c-Myc Antibody (YA497)
  • Experimental Validation Results for c-Myc Antibody (YA497)
  • Experimental Validation Results for c-Myc Antibody (YA497)
  • Experimental Validation Results for c-Myc Antibody (YA497)
  • c-Myc Antibody (YA497) Product Image
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Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:200 1:20

Product Details

Description

c-Myc Antibody (YA497) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to c-Myc.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 45/57-65 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 49 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human c-Myc aa1-50.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102150

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for c-Myc Antibody (YA497)
    Western blot analysis of extracts from Hela (lane 2(20μg)), Raji (lane 3(20μg)), K562 (lane 4(20μg)) and 3T3 (lane 5(20μg)) using c-Myc Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (beta Actin, HY-P83730, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for c-Myc Antibody (YA497)
    HCT 116 cells were seeded at 4*105 cells/well in a 6-well plate and were cultured overnight. Then, cells were treated with 10 and 100 μg/mL Cycloheximide (HY-12320) for 0-8 hours to assess the expression of MDM2 and c-Myc via Western blotting. The results demonstrated that Cycloheximide inhibited the expression of MDM2 and c-Myc in a time-dependent manner. Primary antibody: MDM2 antibody (HY-P83705), c-Myc antibody (HY-P80626), β-actin antibody (HY-P80438).
  • Experimental Validation Results for c-Myc Antibody (YA497)
    HCT 116 cells were seeded at 4*105 cells/well in a 6-well plate and were cultured overnight. Then, cells were treated with 10 μg/mL Cycloheximide (HY-12320) for 0-6 hours to assess the expression of MDM2 and c-Myc via Western blotting. The results demonstrated that Cycloheximide inhibited the expression of MDM2 and c-Myc proteins and showed lot-to-lot consistency. Primary antibody: MDM2 antibody (HY-P83705), c-Myc antibody (HY-P80626), β-actin antibody (HY-P80438).
  • Experimental Validation Results for c-Myc Antibody (YA497)
    Immunocytochemistry analysis of Hela cells labeling c-Myc with c-Myc Antibody (HY-P80626) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with c-Myc Antibody (HY-P80626) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for c-Myc Antibody (YA497)
    Immunocytochemistry analysis of NIH-3T3 cells labeling c-Myc with c-Myc Antibody (HY-P80626) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with c-Myc Antibody (HY-P80626) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    The transcription factor c-Myc is a member of the basic helix-loop-helix leucinezipper (bHLHZip) protein family. The target genes of the c-MYC protein participate in different cellular functions, including cell cycle, survival, protein synthesis, cell adhesion, and micro-RNA expression. c-Myc is also one of the four factors used in reprogramming somatic cells to induce pluripotent stem (iPS) cells and is implicated in maintaining cancer stem-like cells (CSCs) . Most biological functions of c-Myc require heterodimerization with its activation partner Max.

    c-Myc is also part of a dynamic network whose members interact selectively with one another and with various transcriptional coregulators and histone-modifying enzymes. Deregulated expression of c-MYC caused by gene amplification, retroviral insertion, or chromosomal translocation is associated with tumorigenesis. c-Myc has been identified as a highly promising target for cancer therapy.

  • Subcellular Localization

    Nucleus, nucleoplasm; Nucleus, nucleolus; Nucleus; Cytoplasm; Chromosome

  • Isoforms & Post-Translational Modification

    P01106 has 3 isomers: P01106-2: 50565 Da (predicted); P01106-1: 48804 Da (predicted); P01106-3: 50437 Da (predicted).
    Phosphorylated by PRKDC (PubMed:1597196). Phosphorylation at Ser-344 by PIM2 leads to the stabilization of MYC (By similarity). Phosphorylation at Ser-77 by CDK2 prevents Ras-induced senescence (PubMed:19966300, PubMed:20713526). Phosphorylated at Ser-77 by DYRK2; this primes the protein for subsequent phosphorylation by GSK3B at Thr-73 (PubMed:22307329). Phosphorylation at Thr-73 and Ser-77 by GSK3 is required for ubiquitination and degradation by the proteasome (PubMed:15103331, PubMed:17558397, PubMed:8386367). Dephosphorylation at multiple sites by the PNUTS-PP1 complex promotes MYC stability by preventing ubiquitination by the SCF(FBXW7) complex (PubMed:30158517). Dephosphorylation at Ser-77 by protein phosphatase 2A (PPP2CA) promotes its degradation; interaction with PPP2CA is enhanced by AMBRA1 (PubMed:25438055, PubMed:25803737);Ubiquitinated by the SCF(FBXW7) complex when phosphorylated at Thr-73 and Ser-77, leading to its degradation by the proteasome (PubMed:15103331, PubMed:17558397, PubMed:25775507, PubMed:30158517). In the nucleoplasm, ubiquitination is counteracted by USP28, which interacts with isoform 1 of FBXW7 (FBW7alpha), leading to its deubiquitination and preventing degradation (PubMed:17558397, PubMed:17873522). In the nucleolus, however, ubiquitination is not counteracted by USP28 but by USP36, due to the lack of interaction between isoform 3 of FBXW7 (FBW7gamma) and USP28, explaining the selective MYC degradation in the nucleolus (PubMed:17558397, PubMed:25775507). Also polyubiquitinated by the DCX(TRPC4AP) complex (PubMed:20551172, PubMed:29779948). Ubiquitinated by UBR5 when not forming a heterodimer with another bHLH protein, leading to its degradation: UBR5 recognizes and binds a degron that is only available upon heterodimer dissociation (PubMed:33208877, PubMed:37478862). Ubiquitinated by TRIM6 in a phosphorylation-independent manner (By similarity)

  • Subunit

    Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MAX (PubMed:9680483). Interacts with TAF1C and SPAG9. Interacts with PARP10. Interacts with KDM5A and KDM5B. Interacts (when phosphorylated at Thr-73 and Ser-77) with FBXW7 (PubMed:17558397, PubMed:25775507). Interacts with PIM2. Interacts with RIOX1. The heterodimer MYC:MAX interacts with ABI1; the interaction may enhance MYC:MAX transcriptional activity. Interacts with TRIM6 (By similarity). Interacts with NPM1; the binary complex is recruited to the promoter of MYC target genes and enhances their transcription (PubMed:25956029). Interacts with CIP2A; leading to the stabilization of MYC (PubMed:17632056). Interacts with NUP205 (PubMed:22719065). Interacts with HEATR1; the interaction is required for localization of MYC to the nucleolus (PubMed:38225354)

  • SwissProt ID

    P01106

  • Gene ID
    4609 [NCBI]
  • Synonyms

    MYC; BHLHE39; Myc proto-oncogene protein; Class E basic helix-loop-helix protein 39; bHLHe39; Proto-oncogene c-Myc; Transcription factor p64

  • Research Field

    Epigenetics and Nuclear Signaling

c-Myc Antibody (YA497) Related Classifications

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg)), Raji (lane 3(20μg)), K562 (lane 4(20μg)) and 3T3 (lane 5(20μg)) using c-Myc Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (beta Actin, HY-P83730, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     HCT 116 cells were seeded at 4*105 cells/well in a 6-well plate and were cultured overnight. Then, cells were treated with 10 and 100 μg/mL Cycloheximide (HY-12320) for 0-8 hours to assess the expression of MDM2 and c-Myc via Western blotting. The results demonstrated that Cycloheximide inhibited the expression of MDM2 and c-Myc in a time-dependent manner. Primary antibody: MDM2 antibody (HY-P83705), c-Myc antibody (HY-P80626), β-actin antibody (HY-P80438).

  • 1/16

    MedChemExpress Validation
     HCT 116 cells were seeded at 4*105 cells/well in a 6-well plate and were cultured overnight. Then, cells were treated with 10 μg/mL Cycloheximide (HY-12320) for 0-6 hours to assess the expression of MDM2 and c-Myc via Western blotting. The results demonstrated that Cycloheximide inhibited the expression of MDM2 and c-Myc proteins and showed lot-to-lot consistency. Primary antibody: MDM2 antibody (HY-P83705), c-Myc antibody (HY-P80626), β-actin antibody (HY-P80438).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling c-Myc with c-Myc Antibody (HY-P80626) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with c-Myc Antibody (HY-P80626) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of NIH-3T3 cells labeling c-Myc with c-Myc Antibody (HY-P80626) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with c-Myc Antibody (HY-P80626) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • c-Myc Antibody (YA497) Product Image
    1/16
  • Experimental Validation Results for c-Myc Antibody (YA497)
  • Experimental Validation Results for c-Myc Antibody (YA497)
  • Experimental Validation Results for c-Myc Antibody (YA497)
  • Experimental Validation Results for c-Myc Antibody (YA497)
  • Experimental Validation Results for c-Myc Antibody (YA497)
  • c-Myc Antibody (YA497) Product Image
  • Experimental Validation Results for c-Myc Antibody (YA497)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg)), Raji (lane 3(20μg)), K562 (lane 4(20μg)) and 3T3 (lane 5(20μg)) using c-Myc Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (beta Actin, HY-P83730, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for c-Myc Antibody (YA497)
    1/1
    MedChemExpress Validation
     HCT 116 cells were seeded at 4*105 cells/well in a 6-well plate and were cultured overnight. Then, cells were treated with 10 and 100 μg/mL Cycloheximide (HY-12320) for 0-8 hours to assess the expression of MDM2 and c-Myc via Western blotting. The results demonstrated that Cycloheximide inhibited the expression of MDM2 and c-Myc in a time-dependent manner. Primary antibody: MDM2 antibody (HY-P83705), c-Myc antibody (HY-P80626), β-actin antibody (HY-P80438).
  • Experimental Validation Results for c-Myc Antibody (YA497)
    1/1
    MedChemExpress Validation
     HCT 116 cells were seeded at 4*105 cells/well in a 6-well plate and were cultured overnight. Then, cells were treated with 10 μg/mL Cycloheximide (HY-12320) for 0-6 hours to assess the expression of MDM2 and c-Myc via Western blotting. The results demonstrated that Cycloheximide inhibited the expression of MDM2 and c-Myc proteins and showed lot-to-lot consistency. Primary antibody: MDM2 antibody (HY-P83705), c-Myc antibody (HY-P80626), β-actin antibody (HY-P80438).
  • Experimental Validation Results for c-Myc Antibody (YA497)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling c-Myc with c-Myc Antibody (HY-P80626) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with c-Myc Antibody (HY-P80626) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for c-Myc Antibody (YA497)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of NIH-3T3 cells labeling c-Myc with c-Myc Antibody (HY-P80626) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with c-Myc Antibody (HY-P80626) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for c-Myc Antibody (YA497)
  • Experimental Validation Results for c-Myc Antibody (YA497)
  • Experimental Validation Results for c-Myc Antibody (YA497)
  • Experimental Validation Results for c-Myc Antibody (YA497)
  • Experimental Validation Results for c-Myc Antibody (YA497)
  • c-Myc Antibody (YA497) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from Hela (lane 2(20μg)), Raji (lane 3(20μg)), K562 (lane 4(20μg)) and 3T3 (lane 5(20μg)) using c-Myc Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (beta Actin, HY-P83730, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • c-Myc Antibody (YA497) Product Image
    MedChemExpress Validation

    HCT 116 cells were seeded at 4*105 cells/well in a 6-well plate and were cultured overnight. Then, cells were treated with 10 and 100 μg/mL Cycloheximide (HY-12320) for 0-8 hours to assess the expression of MDM2 and c-Myc via Western blotting. The results demonstrated that Cycloheximide inhibited the expression of MDM2 and c-Myc in a time-dependent manner. Primary antibody: MDM2 antibody (HY-P83705), c-Myc antibody (HY-P80626), β-actin antibody (HY-P80438).

  • c-Myc Antibody (YA497) Product Image
    MedChemExpress Validation

    HCT 116 cells were seeded at 4*105 cells/well in a 6-well plate and were cultured overnight. Then, cells were treated with 10 μg/mL Cycloheximide (HY-12320) for 0-6 hours to assess the expression of MDM2 and c-Myc via Western blotting. The results demonstrated that Cycloheximide inhibited the expression of MDM2 and c-Myc proteins and showed lot-to-lot consistency. Primary antibody: MDM2 antibody (HY-P83705), c-Myc antibody (HY-P80626), β-actin antibody (HY-P80438).

  • c-Myc Antibody (YA497) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling c-Myc with c-Myc Antibody (HY-P80626) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with c-Myc Antibody (HY-P80626) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • c-Myc Antibody (YA497) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of NIH-3T3 cells labeling c-Myc with c-Myc Antibody (HY-P80626) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with c-Myc Antibody (HY-P80626) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • c-Myc Antibody (YA497) Product Image WB
  • c-Myc Antibody (YA497) Product Image WB
  • c-Myc Antibody (YA497) Product Image WB
  • c-Myc Antibody (YA497) Product Image ICC
  • c-Myc Antibody (YA497) Product Image ICC