Copy Link Print Information

Beta Actin Antibody (YA823)

(Synonyms: A26C1A antibody; A26C1B antibody; ACTB antibody; ACTB_HUMAN antibody; Actin beta antibody; Actin cytoplasmic 1 antibody; Actin, cytoplasmic 1, N-terminally processed antibody; Actx antibody; b actin antibody; Beta cytoskeletal actin antibody; Beta-actin antibody; BRWS1 antibody; E430023M04Rik antibody; MGC128179 antibody; PS1TP5 binding protein 1 antibody; PS1TP5BP1 antibody; β-actin antibody )
10 Cited Publications
Customer Review

Based on 10 publication(s) in Google Scholar

Beta Actin Antibody is a non-conjugated and Mouse origined IgG1 monoclonal antibody, targeting to Beta Actin. It can be used as a loading control antibody.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, IHC-P, ICC/IF

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 1*PBS (pH7.4), 0.2% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.OR PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

  • Conjugation:
    Non-conjugated
WB
  • Experimental Validation Results for Beta Actin Antibody (YA823)
  • Experimental Validation Results for Beta Actin Antibody (YA823)
  • Experimental Validation Results for Beta Actin Antibody (YA823)
  • Beta Actin Antibody (YA823) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
You May Still Need
Top Publications Citing

Use of MCE Products

Publications Citing Use of MedChemExpress (MCE) Beta Actin Antibody (YA823)

More
10 Publications Citing Use of MCE Beta Actin Antibody (YA823) (3)
WB
  • WB
  • WB
  • WB

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:10000 1:100-1:200 1:50-1:200

Product Details

Description

Beta Actin Antibody is a non-conjugated and Mouse origined IgG1 monoclonal antibody, targeting to Beta Actin. It can be used as a loading control antibody.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 42 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 42 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human Beta-actin.The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

RRID

AB_3102520

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 1*PBS (pH7.4), 0.2% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.OR PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Beta Actin Antibody (YA823)
    HCT 116 cells were seeded at 4*105 cells/well in a 6-well plate and were cultured overnight. Then, cells were treated with 10 and 100 μg/mL Cycloheximide (HY-12320) for 0-8 hours to assess the expression of MDM2 and c-Myc via Western blotting. The results demonstrated that Cycloheximide inhibited the expression of MDM2 and c-Myc in a time-dependent manner. Primary antibody: MDM2 antibody (HY-P83705), c-Myc antibody (HY-P80626), β-actin antibody (HY-P80438).
  • Experimental Validation Results for Beta Actin Antibody (YA823)
    HCT 116 cells were seeded at 4*105 cells/well in a 6-well plate and were cultured overnight. Then, cells were treated with 10 μg/mL Cycloheximide (HY-12320) for 0-6 hours to assess the expression of MDM2 and c-Myc via Western blotting. The results demonstrated that Cycloheximide inhibited the expression of MDM2 and c-Myc proteins and showed lot-to-lot consistency. Primary antibody: MDM2 antibody (HY-P83705), c-Myc antibody (HY-P80626), β-actin antibody (HY-P80438).
  • Experimental Validation Results for Beta Actin Antibody (YA823)
    Western blot analysis of extracts from Hela (lane 2(20μg), HEK293T (lane 3(20μg), Jurkat (lane 4(20μg) using Beta Tubulin (HY-P80487) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80487, 1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells (PubMed:25255767, PubMed:29581253). Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction (PubMed:29581253). In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA (PubMed:29925947). Plays a role in the assembly of the gamma-tubulin ring complex (gTuRC), which regulates the minus-end nucleation of alpha-beta tubulin heterodimers that grow into microtubule protafilaments (PubMed:39321809, PubMed:38609661). Part of the ACTR1A/ACTB filament around which the dynactin complex is built (By similarity). The dynactin multiprotein complex activates the molecular motor dynein for ultra-processive transport along microtubules (By similarity)

  • Subcellular Localization

    Cytoplasm, cytoskeleton; Nucleus

  • Subunit

    Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix (PubMed:16685646, PubMed:28604741). Each actin can bind to 4 others (PubMed:16685646, PubMed:28604741). Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs (PubMed:17289661). Component of the BAF complex, which includes at least actin (ACTB), ARID1A, ARID1B/BAF250, SMARCA2, SMARCA4/BRG1, ACTL6A/BAF53, ACTL6B/BAF53B, SMARCE1/BAF57 SMARCC1/BAF155, SMARCC2/BAF170, SMARCB1/SNF5/INI1, and one or more of SMARCD1/BAF60A, SMARCD2/BAF60B, or SMARCD3/BAF60C (PubMed:18765789). In muscle cells, the BAF complex also contains DPF3 (PubMed:18765789). Found in a complex with XPO6, Ran, ACTB and PFN1 (PubMed:14592989). Interacts with PFN1 (PubMed:10411937, PubMed:25255767). Interacts with XPO6 and EMD (PubMed:15328537). Interacts with ERBB2 (PubMed:21555369). Interacts with GCSAM (PubMed:17823310). Interacts with TBC1D21 (By similarity). Interacts with CPNE1 (via VWFA domain) and CPNE4 (via VWFA domain) (By similarity). Interacts with DHX9 (via C-terminus); this interaction is direct and mediates the attachment to nuclear ribonucleoprotein complexes (PubMed:11687588). Interacts with FAM107A (PubMed:21969592, PubMed:28604741). Associates with the gamma-tubulin ring complex (gTuRC) consisting of TUBGCP2, TUBGCP3, TUBGCP4, TUBGCP5 and TUBGCP6 and gamma-tubulin TUBG1 or TUBG2; within the complex, interacts with TUBGCP3 and TUBGCP6 to form a luminal bridge with MZT1 that stabilizes the initial structure during complex assembly (PubMed:39321809, PubMed:38609661). Part of the ACTR1A/ACTB filament around which the dynactin complex is built (By similarity). The filament contains 8 copies of ACTR1A and 1 ACTB (By similarity). Interacts with TPRN which forms ring-like structures in the stereocilium taper region; the interaction may stabilize stereocilia in inner ear hair cells (By similarity). Interacts with AMOTL2 (via N-terminus), the interaction facilitates binding of cell junction complexes to actin fibers in endothelial cells (By similarity)

  • SwissProt ID

    P60709

  • Gene ID
    60 [NCBI]
  • Synonyms

    A26C1A antibody; A26C1B antibody; ACTB antibody; ACTB_HUMAN antibody; Actin beta antibody; Actin cytoplasmic 1 antibody; Actin, cytoplasmic 1, N-terminally processed antibody; Actx antibody; b actin antibody; Beta cytoskeletal actin antibody; Beta-actin antibody; BRWS1 antibody; E430023M04Rik antibody; MGC128179 antibody; PS1TP5 binding protein 1 antibody; PS1TP5BP1 antibody; β-actin antibody

  • Research Field

    Isotype/Loading Controls

Beta Actin Antibody (YA823) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     HCT 116 cells were seeded at 4*105 cells/well in a 6-well plate and were cultured overnight. Then, cells were treated with 10 and 100 μg/mL Cycloheximide (HY-12320) for 0-8 hours to assess the expression of MDM2 and c-Myc via Western blotting. The results demonstrated that Cycloheximide inhibited the expression of MDM2 and c-Myc in a time-dependent manner. Primary antibody: MDM2 antibody (HY-P83705), c-Myc antibody (HY-P80626), β-actin antibody (HY-P80438).

  • 1/16

    MedChemExpress Validation
     HCT 116 cells were seeded at 4*105 cells/well in a 6-well plate and were cultured overnight. Then, cells were treated with 10 μg/mL Cycloheximide (HY-12320) for 0-6 hours to assess the expression of MDM2 and c-Myc via Western blotting. The results demonstrated that Cycloheximide inhibited the expression of MDM2 and c-Myc proteins and showed lot-to-lot consistency. Primary antibody: MDM2 antibody (HY-P83705), c-Myc antibody (HY-P80626), β-actin antibody (HY-P80438).

  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg), HEK293T (lane 3(20μg), Jurkat (lane 4(20μg) using Beta Tubulin (HY-P80487) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80487, 1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    Beta Actin Antibody (YA823) purchased from MedChemExpress. Usage Cited in: Imeta. 2025 Jul 17;4(5):e70066. [Abstract]

    Customer Validation
     Western blot analysis of IL-22 and β-actin expression in the ileum. β-actin (HY-P80438) was used to normalize the expression of IL-22.

  • 1/16

    Beta Actin Antibody (YA823) purchased from MedChemExpress. Usage Cited in: Discov Oncol. 2025 Apr 9;16(1):499. [Abstract]

    Customer Validation
     CeapinA7 induced S6K dephosphorylation and cellular autophagy protein expression in DLBCL cell lines. Cells were subject to the specified concentrations of ceapinA7, and the expression levels of Bip, phosphorylated S6K, P62, and LC3 proteins were evaluated through Western blot analysis, with β-actin as a loading control. Quantification was based on three independent experiments, with band intensities normalized to the control via ImageJ. Data are displayed in mean ± SD., n = 3.

  • 1/16

    Beta Actin Antibody (YA823) purchased from MedChemExpress. Usage Cited in: Discov Oncol. 2025 Apr 9;16(1):499. [Abstract]

    Customer Validation
     WB detection of changes in LC3-II/β-actin expression after combined application of ceapinA7 and CQ. Results are shown as mean ± SD.

  • Beta Actin Antibody (YA823) Product Image
    1/16
  • Experimental Validation Results for Beta Actin Antibody (YA823)
  • Experimental Validation Results for Beta Actin Antibody (YA823)
  • Experimental Validation Results for Beta Actin Antibody (YA823)
  • Experimental Validation Results for Beta Actin Antibody (YA823)
  • Experimental Validation Results for Beta Actin Antibody (YA823)
  • Experimental Validation Results for Beta Actin Antibody (YA823)
  • Beta Actin Antibody (YA823) Product Image
  • Experimental Validation Results for Beta Actin Antibody (YA823)
    1/1
    MedChemExpress Validation
     HCT 116 cells were seeded at 4*105 cells/well in a 6-well plate and were cultured overnight. Then, cells were treated with 10 and 100 μg/mL Cycloheximide (HY-12320) for 0-8 hours to assess the expression of MDM2 and c-Myc via Western blotting. The results demonstrated that Cycloheximide inhibited the expression of MDM2 and c-Myc in a time-dependent manner. Primary antibody: MDM2 antibody (HY-P83705), c-Myc antibody (HY-P80626), β-actin antibody (HY-P80438).
  • Experimental Validation Results for Beta Actin Antibody (YA823)
    1/1
    MedChemExpress Validation
     HCT 116 cells were seeded at 4*105 cells/well in a 6-well plate and were cultured overnight. Then, cells were treated with 10 μg/mL Cycloheximide (HY-12320) for 0-6 hours to assess the expression of MDM2 and c-Myc via Western blotting. The results demonstrated that Cycloheximide inhibited the expression of MDM2 and c-Myc proteins and showed lot-to-lot consistency. Primary antibody: MDM2 antibody (HY-P83705), c-Myc antibody (HY-P80626), β-actin antibody (HY-P80438).
  • Experimental Validation Results for Beta Actin Antibody (YA823)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg), HEK293T (lane 3(20μg), Jurkat (lane 4(20μg) using Beta Tubulin (HY-P80487) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80487, 1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Beta Actin Antibody (YA823)
  • Experimental Validation Results for Beta Actin Antibody (YA823)
  • Experimental Validation Results for Beta Actin Antibody (YA823)

  • Beta Actin Antibody (YA823) purchased from MedChemExpress. Usage Cited in: Imeta. 2025 Jul 17;4(5):e70066. [Abstract]

    Western blot analysis of IL-22 and β-actin expression in the ileum. β-actin (HY-P80438) was used to normalize the expression of IL-22.

  • Beta Actin Antibody (YA823) purchased from MedChemExpress. Usage Cited in: Discov Oncol. 2025 Apr 9;16(1):499. [Abstract]

    CeapinA7 induced S6K dephosphorylation and cellular autophagy protein expression in DLBCL cell lines. Cells were subject to the specified concentrations of ceapinA7, and the expression levels of Bip, phosphorylated S6K, P62, and LC3 proteins were evaluated through Western blot analysis, with β-actin as a loading control. Quantification was based on three independent experiments, with band intensities normalized to the control via ImageJ. Data are displayed in mean ± SD., n = 3.

  • Beta Actin Antibody (YA823) purchased from MedChemExpress. Usage Cited in: Discov Oncol. 2025 Apr 9;16(1):499. [Abstract]

    WB detection of changes in LC3-II/β-actin expression after combined application of ceapinA7 and CQ. Results are shown as mean ± SD.

  • Beta Actin Antibody (YA823) Product Image
    MedChemExpress Validation

    HCT 116 cells were seeded at 4*105 cells/well in a 6-well plate and were cultured overnight. Then, cells were treated with 10 and 100 μg/mL Cycloheximide (HY-12320) for 0-8 hours to assess the expression of MDM2 and c-Myc via Western blotting. The results demonstrated that Cycloheximide inhibited the expression of MDM2 and c-Myc in a time-dependent manner. Primary antibody: MDM2 antibody (HY-P83705), c-Myc antibody (HY-P80626), β-actin antibody (HY-P80438).

  • Beta Actin Antibody (YA823) Product Image
    MedChemExpress Validation

    HCT 116 cells were seeded at 4*105 cells/well in a 6-well plate and were cultured overnight. Then, cells were treated with 10 μg/mL Cycloheximide (HY-12320) for 0-6 hours to assess the expression of MDM2 and c-Myc via Western blotting. The results demonstrated that Cycloheximide inhibited the expression of MDM2 and c-Myc proteins and showed lot-to-lot consistency. Primary antibody: MDM2 antibody (HY-P83705), c-Myc antibody (HY-P80626), β-actin antibody (HY-P80438).

  • Beta Actin Antibody (YA823) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from Hela (lane 2(20μg), HEK293T (lane 3(20μg), Jurkat (lane 4(20μg) using Beta Tubulin (HY-P80487) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80487, 1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • Beta Actin Antibody (YA823) Product Image WB
  • Beta Actin Antibody (YA823) Product Image WB
  • Beta Actin Antibody (YA823) Product Image WB