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GAPDH Antibody (YA418)

(Synonyms: GAPDH; GAPD; CDABP0047; OK/SW-cl.12; Glyceraldehyde-3-phosphate dehydrogenase; Peptidyl-cysteine S-nitrosylase GAPDH)
31 Cited Publications
Customer Review

Based on 31 publication(s) in Google Scholar

GAPDH Antibody is a non-conjugated and Rabbit origined IgG monoclonal antibody, targeting to GAPDH. It can be used as a loading control antibody.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IHC-P, FC, IP

  • Reactivity :

    Human, Mouse, Rat, Chicken

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P FC ICC
  • Experimental Validation Results for GAPDH Antibody (YA418)
  • Experimental Validation Results for GAPDH Antibody (YA418)
  • Experimental Validation Results for GAPDH Antibody (YA418)
  • Experimental Validation Results for GAPDH Antibody (YA418)
  • Experimental Validation Results for GAPDH Antibody (YA418)
  • Experimental Validation Results for GAPDH Antibody (YA418)
  • Experimental Validation Results for GAPDH Antibody (YA418)
  • Experimental Validation Results for GAPDH Antibody (YA418)
  • Experimental Validation Results for GAPDH Antibody (YA418)
  • Experimental Validation Results for GAPDH Antibody (YA418)
  • GAPDH Antibody (YA418) Product Image
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Top Publications Citing

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31 Publications Citing Use of MCE GAPDH Antibody (YA418) (2)
WB
  • WB
  • WB

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:10000-1:1000000 1:50 1:50 1:50 Use at an assay dependent concentration.

Product Details

Description

GAPDH Antibody is a non-conjugated and Rabbit origined IgG monoclonal antibody, targeting to GAPDH. It can be used as a loading control antibody.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat, Chicken
  • Observed Molecular Weight
    Observed band size: 36 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 36 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Mouse GAPDH.AA range:94-333.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102089

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for GAPDH Antibody (YA418)
    Western blot analysis of extracts from Mouse spleen (lane 2(20μg)) 、HEK293 (lane 3(20μg)) 、Human lung (lane 4(20μg)) and A549 (lane 5(20μg)) using GAPDH Antibody (HY-P80137) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/640000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for GAPDH Antibody (YA418)
    Western blot analysis of extracts from MCF-7(lane 2(20ug) , THP-1(lane 3(20ug)and HepG2(lane 4(20ug) using GAPDH Antibody (HY-P80137) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for GAPDH Antibody (YA418)
    HT-1080 cells were seeded at 60000 cells/well in a 24 well plate and were cultured overnight. Then, cells were treated with Erastin (HY-15763) (0-100 nM) for 24 h, and the expression of GPX-4 was measured. The results indicated that Erastin inhibits GPX-4 expression. Primary antibody: GPX-4 antibody (HY-P80450), GAPDH antibody (HY-P80137).
  • Experimental Validation Results for GAPDH Antibody (YA418)
    HT-1080 cells were seeded at 60000 cells/well in a 24 well plate and were cultured overnight. Then, cells were treated with RSL3 (HY-100218A) (0-20 nM) for 24 h, and the expression of GPX-4 was measured. The results indicated that RSL3 inhibits GPX-4 expression. Primary antibody: GPX-4 antibody (HY-P80450), GAPDH antibody (HY-P80137).
  • Experimental Validation Results for GAPDH Antibody (YA418)
    HCT-116 cells were seeded at 4*105 cells/well in a 6-well plate and were cultured overnight. Then, cells are treated with 10 nM Actinomycin D (HY-17559) for 0-8 hours to assess the expression of MDM2 and p53 via Western blotting. The results demonstrated that Actinomycin D upregulated the expression of MDM2 and p53 in a time-dependent manner. Primary antibody: MDM2 antibody (HY-P83705), p53 antibody (HY-P80938), GAPDH antibody (HY-P80137).
  • Experimental Validation Results for GAPDH Antibody (YA418)
    Immunohistochemical analysis of paraffin-embedded rat liver tissue using GAPDH Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for GAPDH Antibody (YA418)
    Immunohistochemical analysis of paraffin-embedded rat liver tissue using GAPDH Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for GAPDH Antibody (YA418)
    Flow cytometric analysis of 1X10^6 Hela cells labeling GAPDH Antibody (YA418) (HY-P80137, red). Cells were stained with the primary antibody at 1/100 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for GAPDH Antibody (YA418)
    Immunocytochemistry analysis of Hela cells labeling GAPDH with GAPDH Antibody (HY-P80137) at 1:50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with GAPDH Antibody (HY-P80137) at 1:50 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for GAPDH Antibody (YA418)
    Immunocytochemistry analysis of NIH-3T3 cells labeling GAPDH with GAPDH Antibody (HY-P80137) at 1:50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with GAPDH Antibody (HY-P80137) at 1:50 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively (PubMed:11724794, PubMed:3170585). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate (PubMed:11724794, PubMed:3170585). Modulates the organization and assembly of the cytoskeleton (By similarity). Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes (PubMed:23071094). Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation (PubMed:23071094). Also plays a role in innate immunity by promoting TNF-induced NF-kappa-B activation and type I interferon production, via interaction with TRAF2 and TRAF3, respectively (PubMed:23332158, PubMed:27387501). Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis (By similarity). Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity)

  • Subcellular Localization

    Cytoplasm, cytosol; Nucleus; Cytoplasm, perinuclear region; Membrane; Cytoplasm, cytoskeleton

  • Isoforms & Post-Translational Modification

    P04406 has 2 isomers: P04406-1: 36053 Da (predicted); P04406-2: 31548 Da (predicted).
    S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus (By similarity). S-nitrosylation of Cys-247 is induced by interferon-gamma and LDL(ox) implicating the iNOS-S100A8/9 transnitrosylase complex and seems to prevent interaction with phosphorylated RPL13A and to interfere with GAIT complex activity (PubMed:22771119, PubMed:25417112);ISGylated;Sulfhydration at Cys-152 increases catalytic activity;Oxidative stress can promote the formation of high molecular weight disulfide-linked GAPDH aggregates, through a process called nucleocytoplasmic coagulation. Such aggregates can be observed in vivo in the affected tissues of patients with Alzheimer disease or alcoholic liver cirrhosis, or in cell cultures during necrosis. Oxidation at Met-46 may play a pivotal role in the formation of these insoluble structures. This modification has been detected in vitro following treatment with free radical donor (+/-)-(E)-4-ethyl-2-[(E)-hydroxyimino]-5-nitro-3-hexenamide. It has been proposed to destabilize nearby residues, increasing the likelihood of secondary oxidative damages, including oxidation of Tyr-45 and Met-105. This cascade of oxidations may augment GAPDH misfolding, leading to intermolecular disulfide cross-linking and aggregation;Succination of Cys-152 and Cys-247 by the Krebs cycle intermediate fumarate, which leads to S-(2-succinyl)cysteine residues, inhibits glyceraldehyde-3-phosphate dehydrogenase activity. Fumarate concentration as well as succination of cysteine residues in GAPDH is significantly increased in muscle of diabetic mammals. It was proposed that the S-(2-succinyl)cysteine chemical modification may be a useful biomarker of mitochondrial and oxidative stress in diabetes and that succination of GAPDH and other thiol proteins by fumarate may contribute to the metabolic changes underlying the development of diabetes complications;(Microbial infection) Glycosylated by C.rodentium protein NleB, enteropathogenic E.coli protein NleB1 and S.typhimurium protein Ssek1: arginine GlcNAcylation prevents the interaction with TRAF2 and TRAF3 (PubMed:23332158, PubMed:27387501, PubMed:28522607). This leads to reduced ubiquitination of TRAF2 and TRAF3, and subsequent inhibition of NF-kappa-B signaling and type I interferon production, respectively (PubMed:23332158, PubMed:27387501)

  • Subunit

    Homotetramer (PubMed:16239728, PubMed:16510976).

  • SwissProt ID

    P04406

  • Gene ID
    14433 [NCBI]
  • Synonyms

    GAPDH; GAPD; CDABP0047; OK/SW-cl.12; Glyceraldehyde-3-phosphate dehydrogenase; Peptidyl-cysteine S-nitrosylase GAPDH

  • Research Field

    Neuroscience

GAPDH Antibody (YA418) Related Classifications

MOQ
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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Mouse spleen (lane 2(20μg)) 、HEK293 (lane 3(20μg)) 、Human lung (lane 4(20μg)) and A549 (lane 5(20μg)) using GAPDH Antibody (HY-P80137) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/640000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from MCF-7(lane 2(20ug) , THP-1(lane 3(20ug)and HepG2(lane 4(20ug) using GAPDH Antibody (HY-P80137) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     HT-1080 cells were seeded at 60000 cells/well in a 24 well plate and were cultured overnight. Then, cells were treated with Erastin (HY-15763) (0-100 nM) for 24 h, and the expression of GPX-4 was measured. The results indicated that Erastin inhibits GPX-4 expression. Primary antibody: GPX-4 antibody (HY-P80450), GAPDH antibody (HY-P80137).

  • 1/16

    MedChemExpress Validation
     HT-1080 cells were seeded at 60000 cells/well in a 24 well plate and were cultured overnight. Then, cells were treated with RSL3 (HY-100218A) (0-20 nM) for 24 h, and the expression of GPX-4 was measured. The results indicated that RSL3 inhibits GPX-4 expression. Primary antibody: GPX-4 antibody (HY-P80450), GAPDH antibody (HY-P80137).

  • 1/16

    MedChemExpress Validation
     HCT-116 cells were seeded at 4*105 cells/well in a 6-well plate and were cultured overnight. Then, cells are treated with 10 nM Actinomycin D (HY-17559) for 0-8 hours to assess the expression of MDM2 and p53 via Western blotting. The results demonstrated that Actinomycin D upregulated the expression of MDM2 and p53 in a time-dependent manner. Primary antibody: MDM2 antibody (HY-P83705), p53 antibody (HY-P80938), GAPDH antibody (HY-P80137).

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat liver tissue using GAPDH Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat liver tissue using GAPDH Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Flow cytometric analysis of 1X10^6 Hela cells labeling GAPDH Antibody (YA418) (HY-P80137, red). Cells were stained with the primary antibody at 1/100 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling GAPDH with GAPDH Antibody (HY-P80137) at 1:50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with GAPDH Antibody (HY-P80137) at 1:50 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of NIH-3T3 cells labeling GAPDH with GAPDH Antibody (HY-P80137) at 1:50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with GAPDH Antibody (HY-P80137) at 1:50 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    GAPDH Antibody (YA418) purchased from MedChemExpress. Usage Cited in: Food Chem (Oxf). 2025 Oct 8:11:100306. [Abstract]

    Customer Validation
     Effects of CuSO₄ (24 h) on the protein levels of Smad2 (1:1,000), p-Smad2 (Ser250) (1:1,000), Smad3 (1:1,000), and p-Smad3 (Ser423/425) (1:1,000) in fibroblasts. GAPDH was used as a loading control (antibody dilution 1:5,000).

  • 1/16

    GAPDH Antibody (YA418) purchased from MedChemExpress. Usage Cited in: J Transl Med. 2025 Jul 25;23(1):841. [Abstract]

    Customer Validation
     Western blotting analysis of neutrophil protein expression showing phosphorylated Syk (p-Syk), total Syk, and GAPDH expression levels. p-Syk and Syk band intensities were quantified using ImageJ software.

  • GAPDH Antibody (YA418) Product Image
    1/16
  • Experimental Validation Results for GAPDH Antibody (YA418)
  • Experimental Validation Results for GAPDH Antibody (YA418)
  • Experimental Validation Results for GAPDH Antibody (YA418)
  • Experimental Validation Results for GAPDH Antibody (YA418)
  • Experimental Validation Results for GAPDH Antibody (YA418)
  • Experimental Validation Results for GAPDH Antibody (YA418)
  • Experimental Validation Results for GAPDH Antibody (YA418)
  • Experimental Validation Results for GAPDH Antibody (YA418)
  • Experimental Validation Results for GAPDH Antibody (YA418)
  • Experimental Validation Results for GAPDH Antibody (YA418)
  • Experimental Validation Results for GAPDH Antibody (YA418)
  • Experimental Validation Results for GAPDH Antibody (YA418)
  • GAPDH Antibody (YA418) Product Image
  • Experimental Validation Results for GAPDH Antibody (YA418)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Mouse spleen (lane 2(20μg)) 、HEK293 (lane 3(20μg)) 、Human lung (lane 4(20μg)) and A549 (lane 5(20μg)) using GAPDH Antibody (HY-P80137) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/640000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for GAPDH Antibody (YA418)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from MCF-7(lane 2(20ug) , THP-1(lane 3(20ug)and HepG2(lane 4(20ug) using GAPDH Antibody (HY-P80137) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for GAPDH Antibody (YA418)
    1/1
    MedChemExpress Validation
     HT-1080 cells were seeded at 60000 cells/well in a 24 well plate and were cultured overnight. Then, cells were treated with Erastin (HY-15763) (0-100 nM) for 24 h, and the expression of GPX-4 was measured. The results indicated that Erastin inhibits GPX-4 expression. Primary antibody: GPX-4 antibody (HY-P80450), GAPDH antibody (HY-P80137).
  • Experimental Validation Results for GAPDH Antibody (YA418)
    1/1
    MedChemExpress Validation
     HT-1080 cells were seeded at 60000 cells/well in a 24 well plate and were cultured overnight. Then, cells were treated with RSL3 (HY-100218A) (0-20 nM) for 24 h, and the expression of GPX-4 was measured. The results indicated that RSL3 inhibits GPX-4 expression. Primary antibody: GPX-4 antibody (HY-P80450), GAPDH antibody (HY-P80137).
  • Experimental Validation Results for GAPDH Antibody (YA418)
    1/1
    MedChemExpress Validation
     HCT-116 cells were seeded at 4*105 cells/well in a 6-well plate and were cultured overnight. Then, cells are treated with 10 nM Actinomycin D (HY-17559) for 0-8 hours to assess the expression of MDM2 and p53 via Western blotting. The results demonstrated that Actinomycin D upregulated the expression of MDM2 and p53 in a time-dependent manner. Primary antibody: MDM2 antibody (HY-P83705), p53 antibody (HY-P80938), GAPDH antibody (HY-P80137).
  • Experimental Validation Results for GAPDH Antibody (YA418)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat liver tissue using GAPDH Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for GAPDH Antibody (YA418)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat liver tissue using GAPDH Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for GAPDH Antibody (YA418)
    1/1
    MedchemExpress Validation
     Flow cytometric analysis of 1X10^6 Hela cells labeling GAPDH Antibody (YA418) (HY-P80137, red). Cells were stained with the primary antibody at 1/100 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for GAPDH Antibody (YA418)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling GAPDH with GAPDH Antibody (HY-P80137) at 1:50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with GAPDH Antibody (HY-P80137) at 1:50 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for GAPDH Antibody (YA418)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of NIH-3T3 cells labeling GAPDH with GAPDH Antibody (HY-P80137) at 1:50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with GAPDH Antibody (HY-P80137) at 1:50 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for GAPDH Antibody (YA418)
  • Experimental Validation Results for GAPDH Antibody (YA418)
  • Experimental Validation Results for GAPDH Antibody (YA418)
  • Experimental Validation Results for GAPDH Antibody (YA418)
  • Experimental Validation Results for GAPDH Antibody (YA418)
  • Experimental Validation Results for GAPDH Antibody (YA418)
  • Experimental Validation Results for GAPDH Antibody (YA418)
  • Experimental Validation Results for GAPDH Antibody (YA418)
  • Experimental Validation Results for GAPDH Antibody (YA418)
  • Experimental Validation Results for GAPDH Antibody (YA418)

  • GAPDH Antibody (YA418) purchased from MedChemExpress. Usage Cited in: Food Chem (Oxf). 2025 Oct 8:11:100306. [Abstract]

    Effects of CuSO₄ (24 h) on the protein levels of Smad2 (1:1,000), p-Smad2 (Ser250) (1:1,000), Smad3 (1:1,000), and p-Smad3 (Ser423/425) (1:1,000) in fibroblasts. GAPDH was used as a loading control (antibody dilution 1:5,000).

  • GAPDH Antibody (YA418) purchased from MedChemExpress. Usage Cited in: J Transl Med. 2025 Jul 25;23(1):841. [Abstract]

    Western blotting analysis of neutrophil protein expression showing phosphorylated Syk (p-Syk), total Syk, and GAPDH expression levels. p-Syk and Syk band intensities were quantified using ImageJ software.

  • GAPDH Antibody (YA418) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Mouse spleen (lane 2(20μg)) 、HEK293 (lane 3(20μg)) 、Human lung (lane 4(20μg)) and A549 (lane 5(20μg)) using GAPDH Antibody (HY-P80137) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/640000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • GAPDH Antibody (YA418) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from MCF-7(lane 2(20ug) , THP-1(lane 3(20ug)and HepG2(lane 4(20ug) using GAPDH Antibody (HY-P80137) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • GAPDH Antibody (YA418) Product Image
    MedChemExpress Validation

    HT-1080 cells were seeded at 60000 cells/well in a 24 well plate and were cultured overnight. Then, cells were treated with Erastin (HY-15763) (0-100 nM) for 24 h, and the expression of GPX-4 was measured. The results indicated that Erastin inhibits GPX-4 expression. Primary antibody: GPX-4 antibody (HY-P80450), GAPDH antibody (HY-P80137).

  • GAPDH Antibody (YA418) Product Image
    MedChemExpress Validation

    HT-1080 cells were seeded at 60000 cells/well in a 24 well plate and were cultured overnight. Then, cells were treated with RSL3 (HY-100218A) (0-20 nM) for 24 h, and the expression of GPX-4 was measured. The results indicated that RSL3 inhibits GPX-4 expression. Primary antibody: GPX-4 antibody (HY-P80450), GAPDH antibody (HY-P80137).

  • GAPDH Antibody (YA418) Product Image
    MedChemExpress Validation

    HCT-116 cells were seeded at 4*105 cells/well in a 6-well plate and were cultured overnight. Then, cells are treated with 10 nM Actinomycin D (HY-17559) for 0-8 hours to assess the expression of MDM2 and p53 via Western blotting. The results demonstrated that Actinomycin D upregulated the expression of MDM2 and p53 in a time-dependent manner. Primary antibody: MDM2 antibody (HY-P83705), p53 antibody (HY-P80938), GAPDH antibody (HY-P80137).

  • GAPDH Antibody (YA418) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat liver tissue using GAPDH Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • GAPDH Antibody (YA418) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat liver tissue using GAPDH Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • GAPDH Antibody (YA418) Product Image
    MedchemExpress Validation

    Flow cytometric analysis of 1X10^6 Hela cells labeling GAPDH Antibody (YA418) (HY-P80137, red). Cells were stained with the primary antibody at 1/100 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • GAPDH Antibody (YA418) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling GAPDH with GAPDH Antibody (HY-P80137) at 1:50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with GAPDH Antibody (HY-P80137) at 1:50 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • GAPDH Antibody (YA418) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of NIH-3T3 cells labeling GAPDH with GAPDH Antibody (HY-P80137) at 1:50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with GAPDH Antibody (HY-P80137) at 1:50 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • GAPDH Antibody (YA418) Product Image WB
  • GAPDH Antibody (YA418) Product Image WB
  • GAPDH Antibody (YA418) Product Image WB
  • GAPDH Antibody (YA418) Product Image WB
  • GAPDH Antibody (YA418) Product Image WB
  • GAPDH Antibody (YA418) Product Image IHC-P
  • GAPDH Antibody (YA418) Product Image IHC-P
  • GAPDH Antibody (YA418) Product Image FC
  • GAPDH Antibody (YA418) Product Image ICC
  • GAPDH Antibody (YA418) Product Image ICC