1. Apoptosis
  2. Ferroptosis

Erastin 

Cat. No.: HY-15763 Purity: 99.54%
Data Sheet SDS Handling Instructions

Erastin is a ferroptosis activator.

For research use only. We do not sell to patients.
Erastin Chemical Structure

Erastin Chemical Structure

CAS No. : 571203-78-6

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Free Sample (0.5-1 mg)   Apply now  
10 mM * 1 mL in DMSO $120 In-stock
5 mg $100 In-stock
10 mg $150 In-stock
50 mg $400 In-stock
100 mg $650 In-stock
200 mg $1100 In-stock
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  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

Erastin is a ferroptosis activator.

In Vitro

Erastin triggers oxidative, iron-dependent cell death. Treatment of NRAS-mutant HT-1080 fibrosarcoma cells with the RSL molecule erastin (10 µM) results in a time-dependent increase in cytosolic and lipid ROS beginning at 2 hours[1]. Cell death triggered by erastin is significantly inhibited by antioxidants (e.g., α-tocopherol, butylated hydroxytoluene, and β-carotene) and iron chelators (e.g., deferoxamine), suggesting that ROS- and iron-dependent signaling is required for erastin-induced ferroptosis. Erastin can directly bind to VDAC2/3 in BJeLR cells. Knockdown of VDAC2 and VDAC3, but not VDAC1, leads to erastin resistance. Erastin has the ability to reduce glutathione level by directly inhibiting cystine/glutamate antiporter system Xc− activity, with activation of the ER stress response[2]. Erastin potently inhibits HT-29 cell survival. Erastin shows a dose-dependent effect, and 30 μM of erastin displays the most dramatic effect[3].

In Vivo

Intraperitoneal injection of erastin at well-tolerated doses dramatically inhibits HT-29 xenograft growth in severe combined immunodeficient mice[3].

References
Preparing Stock Solutions
Concentration Volume Mass 1 mg 5 mg 10 mg
1 mM 1.8280 mL 9.1401 mL 18.2802 mL
5 mM 0.3656 mL 1.8280 mL 3.6560 mL
10 mM 0.1828 mL 0.9140 mL 1.8280 mL
Please refer to the solubility information to select the appropriate solvent.
Cell Assay
[3]

To test erastin’s activity on colorectal cancer cell survival, HT-29 cells are treated with increasing concentrations of erastin (0.1–30 μM). MTT assay was performed[3]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[3]

Mouse: Mice are treated daily with 10 or 30 mg/kg body weight of erastin (intraperitoneal injection, for 4 weeks) or vehicle control (Saline). Tumor volumes are calculated. Mice body weights are also recorded every week[3]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

547.04

Formula

C₃₀H₃₁ClN₄O₄

CAS No.

571203-78-6

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

Solvent & Solubility

DMSO: 6.4 mg/mL (Need ultrasonic)

* "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

Purity: 99.54%

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Product Name:
Erastin
Cat. No.:
HY-15763
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