2. Apoptosis
  3. Caspase
  4. Z-VAD-FMK

Z-VAD-FMK  (Synonyms: Z-VAD(OH)-FMK)

Cat. No.: HY-16658B Purity: 99.44%
COA Handling Instructions

Z-VAD-FMK (Z-VAD(OH)-FMK) is a well-know pan caspase inhibitor, which does not inhibit ubiquitin carboxy-terminal hydrolase L1 (UCHL1) activity even at concentrations as high as 440 μM.

For research use only. We do not sell to patients.

Z-VAD-FMK Chemical Structure

Z-VAD-FMK Chemical Structure

CAS No. : 161401-82-7

Size Price Stock Quantity
Solution
10 mM * 1 mL in DMSO USD 309 In-stock
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
 USD 309 In-stock
Solid
5 mg USD 310 In-stock
10 mg USD 527 In-stock
50 mg   Get quote  
100 mg   Get quote  

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 229 publication(s) in Google Scholar

Other Forms of Z-VAD-FMK:

Top Publications Citing Use of Products

215 Publications Citing Use of MCE Z-VAD-FMK

Cell Viability Assay
WB
IF
Proliferation Assay

    Z-VAD-FMK purchased from MCE. Usage Cited in: Nat Microbiol. 2022 Jul;7(7):1041-1053.  [Abstract]

    HFFs stably expressing UL37x1 or empty vector are infected with HCMV in the presence of z-VAD-FMK. At 0, 6 and 12 h.

    Z-VAD-FMK purchased from MCE. Usage Cited in: Redox Biol. 2022 Oct;56:102435.  [Abstract]

    Primary HSCs and LX-2 cells are treated with EA (LX-2 cells: 40 μM; primary HSCs: 45 μM), Ferrostatin-1 (1 μM) and Z-VAD-FMK (10 μM), and cells are stained with PI (red fluorescence) to examine the dead cells.

    Z-VAD-FMK purchased from MCE. Usage Cited in: Redox Biol. 2022 Aug;54:102355.

    In CAL-27 cells, Z-VAD-FMK (50 μM; 4 hours) treatment counteracted the NRC-03-induced cytotoxicity and apoptosis.

    Z-VAD-FMK purchased from MCE. Usage Cited in: J Autoimmun. 2022 Sep 13;133:102904.  [Abstract]

    C57BL/6 are treated with Concanavalin A (12 μg/g) alone or zVAD (20 μg/g) alone or zVAD (20 μg/g) + Concanavalin A (12 μg/g) at indicated times. The number of apoptotic cells in the zVAD + Concanavalin A treated group is markedly reduced compared with that observed in the ConA-treated group.

    Z-VAD-FMK purchased from MCE. Usage Cited in: J Autoimmun. 2022 Sep 13;133:102904.  [Abstract]

    The BMDMs are cultured with different concentrations of zVAD (0, 20, 40, 80, 100 μM) for 24 h.

    Z-VAD-FMK purchased from MCE. Usage Cited in: J Autoimmun. 2022 Sep 13;133:102904.  [Abstract]

    After being treated with zVAD (0, 20, 40, 80 μM) for 30 min, the BMDMs are followed by the administration of LPS (100 ng/ml) for 24 h.

    Z-VAD-FMK purchased from MCE. Usage Cited in: Phytother Res. 2022 Oct 17.  [Abstract]

    z-VAD-fmk (5 μM; 4 h) weakens CDBEE-induced apoptosis in MGC-803 and HGC-27 cells.

    Z-VAD-FMK purchased from MCE. Usage Cited in: Adv Sci (Weinh). 2021 Feb 8;8(8):2002874.  [Abstract]

    Representative image and statistical analyses of JC‐1 staining of KYSE30 cells expressing EV or OTUD1 and treated with DDP or Z‐VAD-FMK (50 μM). Z‐VAD-FMK treatment could not completely inhibit the proapoptotic function of OTUD1.

    Z-VAD-FMK purchased from MCE. Usage Cited in: Adv Sci (Weinh). 2021 Feb 8;8(8):2002874.  [Abstract]

    In vitro growth of KYSE30 cells expressing EV or OTUD1 and treated with or without Z‐VAD-FMK (50 μM).

    Z-VAD-FMK purchased from MCE. Usage Cited in: Adv Sci (Weinh). 2021 Feb 8;8(8):2002874.  [Abstract]

    IB further confirmed the activation of the caspase‐dependent apoptotic pathway in OTUD1‐overexpressing cells with AIF ablation, which is diminished by VAD-FMK (50 μM).

    Z-VAD-FMK purchased from MCE. Usage Cited in: Bioact Mater. 2021 Nov 19;13:23-36.  [Abstract]

    H1299 and H460 cells are treated with curcumenol with or without Z-VAD-FMK (10 μM) for 24 h, the cell viability is detected.

    Z-VAD-FMK purchased from MCE. Usage Cited in: Theranostics. 2020 Jun 19;10(17):7710-7729.  [Abstract]

    When the cells were co-treated with Z-VAD-FMK (50 μM; 24 hours), the F-AgÅPs-induced inhibition of survival of 143B and SJSA-1 is significantly attenuated, as revealed by live/dead cell staining.

    Z-VAD-FMK purchased from MCE. Usage Cited in: Acta Biomater. 2020 Jun;109:229-243.  [Abstract]

    The decreased cleaved caspase-3 and Bax proteins showed that Z-VAD successfully inhibited Chemo-PDT induced apoptosis.

    Z-VAD-FMK purchased from MCE. Usage Cited in: Acta Pharm Sin B. 2021 May;11(5):1246-1260.

    Apoptosis of ECa109 and EC9706 cells treated with SFN (40 μM) and Z-VAD-FMK (20 μM) alone or combination for 48 h is analyzed by flow cytometry.

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    Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase Caspase 11 Caspase-13

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Z-VAD-FMK (Z-VAD(OH)-FMK) is a well-know pan caspase inhibitor, which does not inhibit ubiquitin carboxy-terminal hydrolase L1 (UCHL1) activity even at concentrations as high as 440 μM[1].

    IC50 & Target[1]

    Caspase

     

    In Vitro

    Z-VAD-FMK (40 μM) reverses the apoptotic effect exerted by total saponin of Solanum lyratum Thunb (TSSLT) in Hela cells. HeLa cells are pretreated with Z-VAD-FMK (40 μM) for 30 min and exposed to TSSLT (6 μg/mL) for 48 h[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Cell Viability Assay[2]

    Cell Line: HeLa cells
    Concentration: 40 μM
    Incubation Time: Prtreated for 30 minutes
    Result: Prevented TSSLT-induced cell death. More than 80% cell survival was observed.
    Molecular Weight

    453.46

    Appearance

    Solid

    Formula

    C21H28FN3O7
    CAS No.
    SMILES

    O=C(N[[email protected]](C(N[[email protected]@H](C)C(N[[email protected]@H](CC(O)=O)C(CF)=O)=O)=O)C(C)C)OCC1=CC=CC=C1
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, sealed storage, away from moisture and light

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (220.53 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.2053 mL 11.0263 mL 22.0527 mL
    5 mM 0.4411 mL 2.2053 mL 4.4105 mL
    10 mM 0.2205 mL 1.1026 mL 2.2053 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.08 mg/mL (4.59 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: 2.08 mg/mL (4.59 mM); Suspended solution; Need ultrasonic

    • 3.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 2.08 mg/mL (4.59 mM); Clear solution

    *All of the co-solvents are available by MCE.
    Purity & Documentation

    Purity: 99.76%

    COA (245 KB) HNMR (263 KB) LCMS (119 KB)

    Handling Instructions (2659 KB)
    References
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    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    Keywords:

    Z-VAD-FMK161401-82-7Z-VAD(OH)-FMKCaspasepan-caspaseAntiapoptosisHelacellsInhibitorinhibitorinhibit

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