1. Apoptosis
  2. Caspase
  3. Z-VAD-FMK

Z-VAD-FMK (Synonyms: Z-VAD(OH)-FMK)

Cat. No.: HY-16658B Purity: 98.29%
Handling Instructions

Z-VAD-FMK (Z-VAD(OH)-FMK) is a well-know pan caspase inhibitor, which does not inhibit ubiquitin carboxy-terminal hydrolase L1 (UCHL1) activity even at concentrations as high as 440 μM.

For research use only. We do not sell to patients.

Z-VAD-FMK Chemical Structure

Z-VAD-FMK Chemical Structure

CAS No. : 161401-82-7

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 309 In-stock
Estimated Time of Arrival: December 31
1 mg USD 105 In-stock
Estimated Time of Arrival: December 31
5 mg USD 310 In-stock
Estimated Time of Arrival: December 31
10 mg USD 527 In-stock
Estimated Time of Arrival: December 31
50 mg   Get quote  
100 mg   Get quote  

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Customer Review

Based on 2 publication(s) in Google Scholar

Other Forms of Z-VAD-FMK:

Top Publications Citing Use of Products
  • Biological Activity

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Description

Z-VAD-FMK (Z-VAD(OH)-FMK) is a well-know pan caspase inhibitor, which does not inhibit ubiquitin carboxy-terminal hydrolase L1 (UCHL1) activity even at concentrations as high as 440 μM[1].

IC50 & Target[1]

Caspase

 

In Vitro

Z-VAD-FMK (40 μM) reverses the apoptotic effect exerted by total saponin of Solanum lyratum Thunb (TSSLT) in Hela cells. HeLa cells are pretreated with Z-VAD-FMK (40 μM) for 30 min and exposed to TSSLT (6 μg/mL) for 48 h[2].

Cell Viability Assay[2]

Cell Line: HeLa cells
Concentration: 40 μM
Incubation Time: Prtreated for 30 minutes
Result: Prevented TSSLT-induced cell death. More than 80% cell survival was observed.
Molecular Weight

453.46

Formula

C₂₁H₂₈FN₃O₇

CAS No.

161401-82-7

SMILES

O=C(N[[email protected]](C(N[[email protected]@H](C)C(N[[email protected]@H](CC(O)=O)C(CF)=O)=O)=O)C(C)C)OCC1=CC=CC=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (220.53 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.2053 mL 11.0263 mL 22.0527 mL
5 mM 0.4411 mL 2.2053 mL 4.4105 mL
10 mM 0.2205 mL 1.1026 mL 2.2053 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (5.51 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (5.51 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (5.51 mM); Clear solution

*All of the co-solvents are provided by MCE.
References

Purity: 98.29%

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Keywords:

Z-VAD-FMKZ-VAD(OH)-FMKCaspasepan-caspaseAntiapoptosisHelacellsInhibitorinhibitorinhibit

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Z-VAD-FMK
Cat. No.:
HY-16658B
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