1. Apoptosis
  2. Caspase
  3. Z-IETD-FMK

Z-IETD-FMK  (Synonyms: Z-IE(OMe)TD(OMe)-FMK)

Cat. No.: HY-101297 Purity: ≥98.0%
COA Handling Instructions

Z-IETD-FMK (Z-IE(OMe)TD(OMe)-FMK) est un inhibiteur de caspase-8 qui est sélectif et perméable aux cellules. Z-IETD-FMK est également un inhibiteur de granzyme B.

Z-IETD-FMK (Z-IE(OMe)TD(OMe)-FMK) is a selective and cell permeable caspase-8 inhibitor. Z-IETD-FMK is also a granzyme B inhibitor.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Z-IETD-FMK Chemical Structure

Z-IETD-FMK Chemical Structure

CAS No. : 210344-98-2

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
USD 519 In-stock
Solution
10 mM * 1 mL in DMSO USD 519 In-stock
Solid
1 mg USD 168 In-stock
5 mg USD 360 In-stock
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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 37 publication(s) in Google Scholar

Top Publications Citing Use of Products

36 Publications Citing Use of MCE Z-IETD-FMK

WB

    Z-IETD-FMK purchased from MedChemExpress. Usage Cited in: Food Chem Toxicol. 2018 Oct;120:143-154.  [Abstract]

    Z-IETD-FMK remarkably decreases the dioscin-induced up regulation of Caspase-3
    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Z-IETD-FMK (Z-IE(OMe)TD(OMe)-FMK) is a selective and cell permeable caspase-8 inhibitor[1]. Z-IETD-FMK is also a granzyme B inhibitor[5].

    IC50 & Target[1]

    Caspase-8

     

    In Vitro

    T-cellZ-IETD-FMK causes full inhibition only of the proapoptotic effect of TNFα with an IC50 of 0.46 μM[1]. Z-IETD-FMK and Z-VAD-FMK at non-toxic doses are found to be immunosuppressive and inhibit human T cell proliferation induced by mitogens and IL-2. They are shown to block NF-κB in activated primary T cells, but have little inhibitory effect on the secretion of IL-2 and IFN-γ during T cell activation[2]. Z-IETD-FMK inhibits the cleavage of caspase-8 and only partially inhibits the cleavage of caspase-3 and PARP. Z-IETD-FMK can prevent the execution of apoptosis in retinal cells exposed to different apoptotic stimuli[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    In Vivo

    Pharmacological inhibition of caspase-8 by z-IETD-FMK robustly reduces tumor growth and this is closely associated with a reduction in the release of pro-inflammatory cytokines, IL-6, TNF-α, IL-18, IL-1α, IL-33, but not IL-1β. Furthermore, inhibition of caspase-8 reduces the recruitment of innate suppressive cells, such as myeloid-derived suppressor cells, but not of regulatory T cells to lungs of tumor-bearing mice[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Molecular Weight

    654.68

    Formula

    C30H43FN4O11

    CAS No.
    Appearance

    Solid

    Color

    White to light yellow

    Sequence

    Z-Ile-Glu-Thr-Asp-FMK

    Sequence Shortening

    ZIETDFMK

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage

    Sealed storage, away from moisture

    Powder -80°C 2 years
    -20°C 1 year

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    DMSO : 41.67 mg/mL (63.65 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.5275 mL 7.6373 mL 15.2746 mL
    5 mM 0.3055 mL 1.5275 mL 3.0549 mL
    10 mM 0.1527 mL 0.7637 mL 1.5275 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (3.18 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.08 mg/mL (3.18 mM); Clear solution

    • 3.

      Add each solvent one by one:  10% DMSO    90% Corn Oil

      Solubility: ≥ 2.08 mg/mL (3.18 mM); Clear solution

    *All of the co-solvents are available by MedChemExpress (MCE).
    Purity & Documentation

    Purity: ≥98.0%

    References
    Cell Assay
    [2]

    T cell proliferation following mitogen stimulation is determined using [3H]-thymidine incorporation. In brief, PBMCs or purified T cells are seeded at 1×106 cells/mL in 96 well plates and stimulated with either PHA (5 μg/mL or co-stimulated with anti-CD3 mAb (5 μg/mL) and anti-CD28 mAb (2.5 μg/mL) in the presence or absence of caspase inhibitor Z-IETD-FMK. The cells are cultured for 72 h with the last 16 h pulsed with [[3H]-labelled methyl-thymidine (0.037 MBq) prior to harvest onto glass fibre filter mats using a Tomtec automated multi-well harvester[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Administration
    [4]

    Mice: Mice are divided into three groups: (1) naive, non-treated, mice; (2) CTR (control), i.t. instilled with NMU; and (3) lung cancer-bearing mice treated with Z-IETD-FMK (0.5 μg per mouse). The involvement of caspase-8 in lung cancer development is the determined at different time points (3, 7 and 28 days)[4].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References
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    Z-IETD-FMK Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    Product Name:
    Z-IETD-FMK
    Cat. No.:
    HY-101297
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