2. Apoptosis
  3. Caspase
  4. Z-IETD-FMK

Z-IETD-FMK  (Synonyms: Z-IE(OMe)TD(OMe)-FMK)

Cat. No.: HY-101297 Purity: 98.0%
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SDS
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Z-IETD-FMK (Z-IE(OMe)TD(OMe)-FMK) est un inhibiteur de caspase-8 qui est sélectif et perméable aux cellules. Z-IETD-FMK est également un inhibiteur de granzyme B.

Z-IETD-FMK (Z-IE(OMe)TD(OMe)-FMK) is a selective and cell permeable caspase-8 inhibitor. Z-IETD-FMK is also a granzyme B inhibitor.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

CAS No. : 210344-98-2

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Customer Review

Based on 68 publication(s) in Google Scholar

Other Forms of Z-IETD-FMK:

Z-IETD-FMK Related Antibodies

Top Publications Citing Use of Products

68 Publications Citing Use of MCE Z-IETD-FMK

2D/3D Cell Culture and Differentiation
WB
Cell Imaging/Staining

    Z-IETD-FMK purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2025 Jun 17;16(1):452.  [Abstract]

    Representative phase-contrast images of pyroptotic cell death induced by RG7388 in H23 cells pretreated with or without pan-caspase inhibitor Z-VAD-FMK (zVAD, 50 μM), caspase-8 inhibitor Z-IETD-FMK (Cas8i, 50 μM) or caspase-3 inhibitor Z-DEVD-FMK (Cas3i, 25 μM) as indicated for 6 hours

    Z-IETD-FMK purchased from MedChemExpress. Usage Cited in: Cell. 2024 Dec 26;187(26):7533-7550.e23.  [Abstract]

    Organoids were treated with Z-IETD-FMK (Z-IETD, 40 μM, 16 h) during rIFN-λ treatment.

    Z-IETD-FMK purchased from MedChemExpress. Usage Cited in: Cell Mol Immunol. 2023 Oct;20(10):1203-1215.  [Abstract]

    THP-1 monocytes were pretreated for 0.5 h with DMSO or Z-IETD-FMK (25 μM) and stimulated for 6 h with heat-killed gram-negative bacteria at twice the number of cells. Immunoblots showing caspase-8, caspase-1 and IL-1β in total cell lysate are representative of one of two experimental repeats.

    Z-IETD-FMK purchased from MedChemExpress. Usage Cited in: Cell Mol Immunol. 2023 Oct;20(10):1203-1215.  [Abstract]

    PBMCs were pretreated for 0.5 h with DMSO or Z-IETD-FMK (25 μM) and stimulated for 16 h with heat-killed gram-negative bacteria at twice the number of monocytes.

    Z-IETD-FMK purchased from MedChemExpress. Usage Cited in: Cell Mol Immunol. 2023 Oct;20(10):1203-1215.  [Abstract]

    THP-1 monocytes were pretreated for 0.5 h with DMSO or Z-IETD-FMK (25 μM) and stimulated at a specific time point with heat-killed E. coli XL10 at twice the number of cells. Immunoblots showing P-TAK1, caspase-8 and caspase-1 in total cell lysate are representative of one of two experimental repeats.

    Z-IETD-FMK purchased from MedChemExpress. Usage Cited in: Food Chem Toxicol. 2018 Oct:120:143-154.  [Abstract]

    Z-IETD-FMK remarkably decreases the dioscin-induced up regulation of Caspase-3

    View All Caspase Isoform Specific Products:

    View All Isoforms
    Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase Caspase 11 Caspase-13

    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Z-IETD-FMK (Z-IE(OMe)TD(OMe)-FMK) is a selective and cell permeable caspase-8 inhibitor[1]. Z-IETD-FMK is also a granzyme B inhibitor[5].

    IC50 & Target[1]

    Caspase-8

     

    In Vitro

    T-cellZ-IETD-FMK causes full inhibition only of the proapoptotic effect of TNFα with an IC50 of 0.46 μM[1]. Z-IETD-FMK and Z-VAD-FMK at non-toxic doses are found to be immunosuppressive and inhibit human T cell proliferation induced by mitogens and IL-2. They are shown to block NF-κB in activated primary T cells, but have little inhibitory effect on the secretion of IL-2 and IFN-γ during T cell activation[2]. Z-IETD-FMK inhibits the cleavage of caspase-8 and only partially inhibits the cleavage of caspase-3 and PARP. Z-IETD-FMK can prevent the execution of apoptosis in retinal cells exposed to different apoptotic stimuli[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Z-IETD-FMK Related Antibodies
    In Vivo

    Pharmacological inhibition of caspase-8 by z-IETD-FMK robustly reduces tumor growth and this is closely associated with a reduction in the release of pro-inflammatory cytokines, IL-6, TNF-α, IL-18, IL-1α, IL-33, but not IL-1β. Furthermore, inhibition of caspase-8 reduces the recruitment of innate suppressive cells, such as myeloid-derived suppressor cells, but not of regulatory T cells to lungs of tumor-bearing mice[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Molecular Weight

    654.68

    Formula

    C30H43FN4O11
    CAS No.
    Appearance

    Solid

    Color

    White to light yellow

    Sequence

    Z-Ile-Glu-Thr-Asp-FMK
    Sequence Shortening

    ZIETDFMK
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    Sealed storage, away from moisture

    Powder -80°C 2 years
    -20°C 1 year

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    DMSO : 41.67 mg/mL (63.65 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.5275 mL 7.6373 mL 15.2746 mL
    5 mM 0.3055 mL 1.5275 mL 3.0549 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

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    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    Purity & Documentation

    Purity: ≥98.0%

    SDS (396 KB)

    COA (248 KB) HNMR (244 KB)

    Handling Instructions (2659 KB)
    References
    Cell Assay
    [2]

    T cell proliferation following mitogen stimulation is determined using [3H]-thymidine incorporation. In brief, PBMCs or purified T cells are seeded at 1×106 cells/mL in 96 well plates and stimulated with either PHA (5 μg/mL or co-stimulated with anti-CD3 mAb (5 μg/mL) and anti-CD28 mAb (2.5 μg/mL) in the presence or absence of caspase inhibitor Z-IETD-FMK. The cells are cultured for 72 h with the last 16 h pulsed with [[3H]-labelled methyl-thymidine (0.037 MBq) prior to harvest onto glass fibre filter mats using a Tomtec automated multi-well harvester[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Animal Administration
    [4]

    Mice: Mice are divided into three groups: (1) naive, non-treated, mice; (2) CTR (control), i.t. instilled with NMU; and (3) lung cancer-bearing mice treated with Z-IETD-FMK (0.5 μg per mouse). The involvement of caspase-8 in lung cancer development is the determined at different time points (3, 7 and 28 days)[4].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.5275 mL 7.6373 mL 15.2746 mL 38.1866 mL
    5 mM 0.3055 mL 1.5275 mL 3.0549 mL 7.6373 mL
    10 mM 0.1527 mL 0.7637 mL 1.5275 mL 3.8187 mL
    15 mM 0.1018 mL 0.5092 mL 1.0183 mL 2.5458 mL
    20 mM 0.0764 mL 0.3819 mL 0.7637 mL 1.9093 mL
    25 mM 0.0611 mL 0.3055 mL 0.6110 mL 1.5275 mL
    30 mM 0.0509 mL 0.2546 mL 0.5092 mL 1.2729 mL
    40 mM 0.0382 mL 0.1909 mL 0.3819 mL 0.9547 mL
    50 mM 0.0305 mL 0.1527 mL 0.3055 mL 0.7637 mL
    60 mM 0.0255 mL 0.1273 mL 0.2546 mL 0.6364 mL
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    Z-IETD-FMK Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Z-IETD-FMK210344-98-2Z-IE(OMe)TD(OMe)-FMKCaspaseInhibitorinhibitorinhibit

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