2. Academic Validation
  3. Mechanism of Juglone-Induced Cell Cycle Arrest and Apoptosis in Ishikawa Human Endometrial Cancer Cells

Mechanism of Juglone-Induced Cell Cycle Arrest and Apoptosis in Ishikawa Human Endometrial Cancer Cells

  • J Agric Food Chem. 2019 Jul 3;67(26):7378-7389. doi: 10.1021/acs.jafc.9b02759.
Yuan-Yuan Zhang 1,  Fan Zhang 1,  Ying-Shuo Zhang 1,  Kiran Thakur 1,  Jian-Guo Zhang 1,  Yun Liu 2,  Huan Kan 2,  Zhao-Jun Wei 1 3
Affiliations

Affiliations

  • 1 School of Food and Biological Engineering , Hefei University of Technology , Hefei 230009 , People's Republic of China.
  • 2 Key Laboratory for Forest Resources Conservation and Utilization in the Southwest Mountains of China, Ministry of Education , Southwest Forestry University , Kunming 650224 , People's Republic of China.
  • 3 Anhui Province Key Laboratory of Functional Compound Seasoning , Anhui Qiangwang Seasoning Food Company, Ltd. , Jieshou 236500 , People's Republic of China.
PMID: 31184118 DOI: 10.1021/acs.jafc.9b02759
Abstract

The molecular mechanism of Juglone-induced cell cycle arrest and Apoptosis in human endometrial Cancer cells was investigated. Juglone was purified from the green husk of Carya cathayensis Sarg and identified by HPLC, LC-MS/MS, and NMR. At an IC50 of 20.81 μM, juglone significantly inhibited Ishikawa cell proliferation, as shown by S phase arrest mediated by inactivation of cyclin A protein ( p < 0.05). The ROS levels increased significantly after exposure to juglone, which paralleled increases in the mRNA and protein expression of p21 and decreases in the levels of CDK2, cdc25A, Chk1, and cyclin A. The expression of Bcl-2 and Bcl-xL was significantly down-regulated, whereas the expression of Bax, Bad and cyto c was up-regulated, and we later confirmed the involvement of the mitochondrial pathway in juglone-induced Apoptosis. Our in vitro results stated that juglone can be studied further as an effective natural Anticancer agent.

Keywords

Sarg; Ishikawa; apoptosis; cell cycle; juglone; reactive oxygen species.

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