Ac-DEVD-AMC
Based on 5 publication(s) in Google Scholar
Ac-DEVD-AMC is a fluorescent substrate of caspase-3/caspase-7. When treating Ac-DEVD-AMC with cell lysate, Ac-DEVD-AMC releases amino-4-methylcoumarin (AMC) for fluorescence detection, with an excitation wavelength of 380 nm and an emission wavelength of 460 nm.
For research use only. We do not sell to patients.
- Purity: 99.18%
- CAS No.: 169332-61-0
- Formula: C30H37N5O13
- Molecular Weight:675.64
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Storage:
Sealed storage, away from moisture and light.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Ac-DEVD-AMC
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Biological Activity
Guide (The following is our recommended solution. This solution is merely a guideline and should be modified according to your specific needs.)
Ac-DEVD-AMC Fluorescence Labeling[2]
(1) Preparation of caspase buffer: 0.5% Igepal CA-630, 10 mM HEPES (pH 7.4), 2 mM EDTA, 0.5 mM phenylmethylsulfonyl fluoride (PMSF), and 5 μg/mL leucine enkephalin.
(2) PC12 cells were treated with 50 μM 6-OHDA for 20 hours.
(3) Centrifuge at 250 g for 5 minutes to collect cells, wash with PBS, and centrifuge again at 250 g for 5 minutes.
(4) Resuspend the cells in caspase buffer at a concentration of 2 uL/10^5 cells and incubate at 4°C for 20 minutes for cell lysis.
(5) Collect the lysates by centrifugation at 7200 g, 4°C, and for 10 minutes.
(6) Incubate 20 μL of the lysate with 100 μM Ac-DEVD-AMC at 30°C for 10 minutes.
(7) Measure caspase activity using a fluorescence spectrophotometer at an excitation wavelength of 380 nm and an emission wavelength of 460 nm.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
480
390
Chemical Information
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CAS No. 169332-61-0
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Appearance Solid
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Molecular Weight 675.64
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Formula C30H37N5O13
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Color White to off-white
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Sequence
N-Acetyl-Asp-Glu-Val-Asp-7-amido-4-Methylcoumarin
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Sequence Shortening
Ac-DEVD-7-amido-4-Methylcoumarin
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture and light
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (5)
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Journal Impact Factor
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Most Recent
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Nucleic Acids Res
EWS-FLI1 modulated alternative splicing of ARID1A reveals novel oncogenic function through the BAF complex. [Abstract]2019 Oct 10;47(18):9619-9636. PMID: 31392992 -
Research (Wash D C)
Natural Flavonoids Genistein and Baicalein as Well-Tolerated Radiosensitizers to Enhance the Efficacy of 177Lu-PSMA617 in Prostate Cancer: In Vitro and In Vivo Studies. [Abstract]2026 Jun 10:9:1314. PMID: 42281770 -
Cell Death Dis
TNF-α synergises with IFN-γ to induce caspase-8-JAK1/2-STAT1-dependent death of intestinal epithelial cells. [Abstract]2021 Sep 23;12(10):864. PMID: 34556638 -
J Agric Food Chem
Mechanism of Juglone-Induced Cell Cycle Arrest and Apoptosis in Ishikawa Human Endometrial Cancer Cells. [Abstract]2019 Jul 3;67(26):7378-7389. PMID: 31184118 -
PLoS One
2019 Dec 30;14(12):e0227278. PMID: 31887216
Solvent & Solubility
DMSO : 10 mg/mL (14.80 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : < 0.1 mg/mL (insoluble)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (278 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Nobel CS, et al. Disulfiram is a potent inhibitor of proteases of the caspase family. Chem Res Toxicol. 1997 Dec;10(12):1319-24. [Content Brief]
[2]. Ochu EE, et al. Caspases mediate 6-hydroxydopamine-induced apoptosis but not necrosis in PC12 cells. J Neurochem. 1998 Jun;70(6):2637-40. [Content Brief]
[3]. Yakovlev AA, Gorokhovatsky AY, Onufriev MV, Beletsky IP, Gulyaeva NV. Brain cathepsin B cleaves a caspase substrate. Biochemistry (Mosc). 2008 Mar;73(3):332-6. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.4801 mL | 7.4004 mL | 14.8008 mL | 37.0020 mL |
| 5 mM | 0.2960 mL | 1.4801 mL | 2.9602 mL | 7.4004 mL | |
| 10 mM | 0.1480 mL | 0.7400 mL | 1.4801 mL | 3.7002 mL |
Ac-DEVD-AMC Related Classifications
HY-P1003 Related Classifications
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