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Ac-DEVD-AMC 

Cat. No.: HY-P1003 Purity: 99.93%
Handling Instructions

Ac-DEVD-AMC is the Caspase-3 substrate.

For research use only. We do not sell to patients.

Ac-DEVD-AMC Chemical Structure

Ac-DEVD-AMC Chemical Structure

CAS No. : 169332-61-0

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1 mg USD 60 In-stock
Estimated Time of Arrival: December 31
5 mg USD 180 In-stock
Estimated Time of Arrival: December 31
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Customer Review

Based on 2 publication(s) in Google Scholar

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Description

Ac-DEVD-AMC is the Caspase-3 substrate.

In Vitro

Ac-DEVD-AMC is the caspase-3 substrate and could be used to monitor intracellular caspase-3 activity[1].The AMC moiety is highest at 2 h (area =5.6±1.8), the AMC peak area for Ac-DEVD-AMC incubated without lung specimen (substrate alone) for 6 h is relatively small[2].

Molecular Weight

675.64

Formula

C₃₀H₃₇N₅O₁₃

CAS No.

169332-61-0

Sequence

N-Acetyl-Asp-Glu-Val-Asp-7-amido-4-Methylcoumarin

Sequence Shortening

Ac-DEVD-7-amido-4-Methylcoumarin

SMILES

CC(C1=CC=C(NC([[email protected]](CC(O)=O)NC([[email protected]](C(C)C)NC([[email protected]](CCC(O)=O)NC([[email protected]@H](NC(C)=O)CC(O)=O)=O)=O)=O)=O)C=C1O2)=CC2=O

Shipping

Room temperature in continental US; may vary elsewhere

Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

H2O : < 0.1 mg/mL (insoluble)

References
Cell Assay
[2]

Lung fragments (about 20 mg each) are collected from rats and mice. The samples are incubated at 37°C in 50 mL KH buffer or MEM (continuously gass with 95% O2 : 5% CO2) for up to 6 h. At specific time points, samples are incubated in oxygenated KH buffer or MEM with 32 μM zVAD-fmk or 15 μL DMSO for 20 min (f/v=1.0 mL). Ac-DEVD-AMC (37 μM) is then added and the incubation continues for additional 20 min. At the end of incubation, the tissue is disrupted by vigorous homogenization for 2 min, sonication for 3 min and 10 passages through a 27-G needle. This disruption procedure quenches the Ac-DEVD-AMC cleavage reaction due to dilution. The supernatants are collected by centrifugation (~6,300g for 90 min) through microcentrifuge filter, separateed on HPLC, and analyzed for the fluorogenic AMC moiety[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

Purity: 99.93%

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Ac-DEVD-AMC
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