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  3. Ac-DEVD-AMC

Ac-DEVD-AMC is a fluorescent substrate of caspase-3/caspase-7. When treating Ac-DEVD-AMC with cell lysate, Ac-DEVD-AMC releases amino-4-methylcoumarin (AMC) for fluorescence detection, with an excitation wavelength of 380 nm and an emission wavelength of 460 nm.

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Custom Peptide Synthesis

Ac-DEVD-AMC Chemical Structure

Ac-DEVD-AMC Chemical Structure

CAS No. : 169332-61-0

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Based on 4 publication(s) in Google Scholar

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Description

Ac-DEVD-AMC is a fluorescent substrate of caspase-3/caspase-7. When treating Ac-DEVD-AMC with cell lysate, Ac-DEVD-AMC releases amino-4-methylcoumarin (AMC) for fluorescence detection, with an excitation wavelength of 380 nm and an emission wavelength of 460 nm[1][2][3].

In Vitro

Ac-DEVD-AMC fluorescence labeling[2]
(1) Preparation of caspase buffer: 0.5% Igepal CA-630, 10 mM HEPES (pH 7.4), 2 mM EDTA, 0.5 mM phenylmethylsulphonyl fluoride (PMSF) and 5 μg/mL leupeptin.
(2) PC12 cells were treated with 50 μM 6-OHDA for 20 h.
(3) Collect cells by centrifugation at 250 g for 5 min, wash with PBS, and then centrifuge at 250 g for 5 min.
(4) Resuspension cells with caspase buffer at a ratio of 2 μL/105 cells and lyse at 4°C for 20 min.
(5) Collect the lysate by centrifugation at 7200 g, 4°C and 10 min.
(6) Incubate 20 μL lysate with 100 μM Ac-DEVD-AMC at 30°C for 10 min.
(7) Measure caspase activity using a fluorescence spectrophotometer at an excitation wavelength of 380 nm and an emission wavelength of 460 nm.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

675.64

Formula

C30H37N5O13

CAS No.
Appearance

Solid

Color

White to off-white

Emission (Em)

480

Excitation (Ex)

390

Sequence

N-Acetyl-Asp-Glu-Val-Asp-7-amido-4-Methylcoumarin

Sequence Shortening

Ac-DEVD-7-amido-4-Methylcoumarin

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture and light

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

H2O : < 0.1 mg/mL (insoluble)

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This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 3.33 mg/mL (4.93 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
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Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.93%

References
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Ac-DEVD-AMC Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Ac-DEVD-AMC
Cat. No.:
HY-P1003
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