- Fluorescent Dyes
- Fluorescence Wavelength ranges
- Excitation Wavelength (Ex)
- Ex 381-450 nm Violet
Ex 381-450 nm Violet
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Ex 381-450 nm Violet (47)
- Formula: C40H53N5O10
- Molecular Weight: 763.88
Suc-Leu-Leu-Val-Tyr-AMC is a membrane-permeable calpain-specific fluorogenic substrate (Ex/Em = 390/480 nm).
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- Formula: C10H11ClN2O2
- Molecular Weight: 226.66
Monochlorobimane (Chlorobimane) is a fluorescent dye (λex=395 nm, λem=490 nm) to measure glutathione (GSH) in cellular assays.
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- Formula: C13H9Li2N5O9S2
- Molecular Weight: 457.25
Luc Yellow CH dilithium is a high-intensity fluorescent probe containing free hydrazyl groups. Luc Yellow CH can react with fatty aldehydes at room temperature. Luc Yellow CH serves as a biological tracer to monitor neuronal branching, regeneration, gap junction detection and characterization, and selective ablation of cells after aldehyde fixation. Luc Yellow CH displays the maximum excitation/emission of 430 nm/540 nm, respectively.
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- Formula: C20H18N2O2S
- Molecular Weight: 350.44
Coumarin 6, a fluorescent dye, is used as a fluorescent probe in a microparticle drug delivery system to conduct in vivo tracking, cell uptake, and transport mechanism studies of drug delivery systems (λexc=450 nm, λem=505 nm).
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- Formula: C13H9K2N5O9S2
- Molecular Weight: 521.57
Lucifer Yellow CH dipotassium is a high-intensity fluorescent probe containing free hydrazyl groups. Lucifer Yellow CH can react with fatty aldehydes at room temperature. Lucifer Yellow CH serves as a biological tracer to monitor neuronal branching, regeneration, gap junction detection and characterization, and selective ablation of cells after aldehyde fixation. Lucifer yellow CH displays the maximum excitation/emission of 430 nm/540 nm, respectively.
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- Formula: C26H21F5N4O4S
- Molecular Weight: 580.53
ER-Tracker dye is a derivative of BODIPY series dyes coupled with Glibenclamide (HY-15206), highly selective binding to the endoplasmic reticulum, non-toxic to cells at low concentrations, this type of dye is an environmentally sensitive probe, and formaldehyde treatment can still retain part of the fluorescence, with high fluorescence life, good extinction coefficient and other characteristics. Glibenclamide is an atp-dependent K+ channel blocker (Kir6, KATP) and CFTR Cl-channel blocker that binds in the endoplasmic reticulum. ER-Tracker is not suitable for staining fixed cells. Staining followed by fixation is possible, but cells fixed with aldehydes will only retain partial fluorescence (Ex/Em = 374/ 430-640 nm).
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- Formula: C16H7Na3O10S3
- Molecular Weight: 524.39
Pyranine (HPTS; Solvent Green 7) is a cell-impermeant pH-sensitive fluorescent indicator. Pyranine acts as a class of fluorescent chemosensor for the Cu+ ion. Pyranine is used as a coloring agent, biological stain, optical detecting reagent, and pH indicator (Ex/Em = 450/510 nm).
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- Formula: C28H33N7O7
- Molecular Weight: 579.60
Z-Gly-Gly-Arg-AMC is a thrombin-specific fluorogenic substrate for testing of thrombin generation in PRP and platelet-poor plasma (PPP) (Ex/Em = 390/480 nm).
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- Formula: C30H37N5O13
- Molecular Weight: 675.64
Ac-DEVD-AMC is a fluorescent substrate of caspase-3/caspase-7. When treating Ac-DEVD-AMC with cell lysate, Ac-DEVD-AMC releases amino-4-methylcoumarin (AMC) for fluorescence detection, with an excitation wavelength of 380 nm and an emission wavelength of 460 nm.
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- Formula: C30H37N7O9
- Molecular Weight: 639.66
Z-Gly-Gly-Arg-AMC acetate is a thrombin-specific fluorogenic substrate for testing of thrombin generation in PRP and platelet-poor plasma (PPP) (Ex/Em = 390/480 nm).
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- Formula: C32H34B2O7
- Molecular Weight: 552.23
Peroxyfluor 1 is a cell-permeable probe for H2O2. Peroxyfluor 1 represents a first-generation, green-fluorescent probe.
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- Formula: C9H10ClN5O5
- Molecular Weight: 303.66
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- Formula: C10H5ClF2O3
- Molecular Weight: 246.59
CellTracker Blue CMF2HC Dye is a blue dye, can be used in two-channel nuclei acid sequencing, with blue and purple excitation light (450-460 nm/400-405nm or 415-450 nm/480-525nm). CellTracker Blue CMF2HC Dye can be used to rapid determination of antibiotic sensitivity of microorganisms.
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- Formula: C20H18Cl2N4O2
- Molecular Weight: 417.29
True Blue (NCI 240899) is a fluorescent dye, as neuronal retrograde tracer (excitation wavelength 395-425 nm, barrier filter 450 nm). True Blue can label neuron and has no effects on neuronal survival.
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- Formula: C32H37N5O6
- Molecular Weight: 587.67
Ac-WLA-AMC is a specific 20S constitutive proteasome β5 fluorogenic substrate.
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- Formula: C16H18O8
- Molecular Weight: 338.31
4-Methylumbelliferyl β-D-galactopyranoside is a fluorescent substrate for β-galactosidase which, when cleaved, produces a water-soluble blue fluorescent coumarin fluorophore that can be detected using a fluoroenzymeter or fluorometer.
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- Formula: C33H38F3N7O11
- Molecular Weight: 765.69
Ac-LEHD-AFC is a fluorescent substrate for caspase-9, can be used to assess the activity of caspase.
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- Formula: C21H29ClN4O6
- Molecular Weight: 468.93
D-Ala-Lys-AMCA hydrochloride is a known proton-coupled oligopeptide transporter 1 (PEPT1) substrate that emits blue fluorescence. D-Ala-Lys-AMCA hydrochloride may be transported into liver cancer cells and Caco-2 cells based on fluorescence analysis. D-Ala-Lys-AMCA hydrochloride can be used for characterizing PEPT1-specific substrates or inhibitors (Ex/Em = 390/480 nm).
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- Formula: C16H18N2O4S
- Molecular Weight: 334.39
Phenazine ethosulfate is a cationic dye (Ex=390 nm, Em=530 nm) and electron acceptor that can be used in dye-linked enzyme assays. Phenazine ethosulfate exhibits weak, pH-dependent fluorescence properties and serves as an oxidative substrate. Phenazine ethosulfate promotes the reduction of equine heart metmyoglobin to ferrohemoglobin. As an intermediate, Phenazine ethosulfate can be used in combination with ascorbic acid for the detection of nitric oxide reductase activity.
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- Formula: C16H8Na2O8S2
- Molecular Weight: 438.34
DHPDS disodium salt is a pH-sensitive fluorescent dye. DHPDS disodium salt is used to measure intracellular pH (pHi) from the surface fluorescence of the isolated perfused rat liver.
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