Z-Gly-Gly-Arg-AMC acetate
Based on 11 publication(s) in Google Scholar
Z-Gly-Gly-Arg-AMC acetate is a thrombin-specific fluorogenic substrate for testing of thrombin generation in PRP and platelet-poor plasma (PPP) (Ex/Em = 390/480 nm).
For research use only. We do not sell to patients.
- Purity: 99.78%
- CAS No.: 2070009-61-7
- Formula: C30H37N7O9
- Molecular Weight:639.66
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Storage:
Sealed storage, away from moisture and light.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Z-Gly-Gly-Arg-AMC acetate
More- Cell Rep Med. 2025 Aug 19;6(8):102260. [Abstract]
- Acta Pharmacol Sin. 2024 Nov;45(11):2300-2312. [Abstract]
- Int J Biol Macromol. 2019 Sep 15:137:261-269. [Abstract]
- Int J Biol Macromol. 2019 Aug 1:134:622-630. [Abstract]
- Blood Adv. 2025 Jun 10;9(11):2695-2705. [Abstract]
- J Mater Chem B. 2017 Oct 14;5(38):7876-7884. [Abstract]
- RSC Adv. 2020 Jan 22;10(7):3626-3635. [Abstract]
- Chem Biol Interact. 2023 Jun 1:378:110501. [Abstract]
- Bioorg Chem. 2019 Nov:92:103199. [Abstract]
- Spectrochim Acta A Mol Biomol Spectrosc. 2022 Jan 5:264:120250. [Abstract]
- Phytochemistry. 2019 Sep:165:112025. [Abstract]
Biological Activity
Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
1. Solution preparation[3]
1.1 Preparation of stock solution
Solvent: H2O
Concentration: 10 mM.
Storage: Store at -20°C or -80°C in dark after aliquoting. Avoid repeated freezing and thawing.
1.2 Preparation of working solution
Dilute to 10 μM with PBS (or other buffer) (optimized according to the experiment).
Note: The working solution should be prepared and used immediately. Keep it away from light.
2. Thrombin inhibition screen
2.1 The samples and thrombin are transferred to a 384-well plate in sequence.
2.2 Add Z-Gly-Gly-Arg-AMC (50 μM) after incubation for 15 minutes.
2.3 Using a buffer containing 10 mM Tris-Cl (pH 7.4), 150 mM NaCl, 10 mM MgCl2, 1 mM CaCl2, 0.1% w/v BSA, 0.01% v/v Triton-X100, and 1% v/v DMSO and the fluorogenic substrate Z-Gly-Gly-Arg-AMC (50 μM).
2.4 The fluorescence intensity is measured with a fluorescence plate reader (Ex/Em = 360/465 nm) at 25°C for a period of 30 min with a read every 3 min.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
480
390
Chemical Information
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CAS No. 2070009-61-7
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Appearance Solid
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Molecular Weight 639.66
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Formula C30H37N7O9
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Color White to off-white
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Sequence
Z-Gly-Gly-Arg-AMC
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Sequence Shortening
ZGGR-AMC
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture and light
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (11)
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Journal Impact Factor
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Most Recent
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Cell Rep Med
High-fat diet increases circulating palmitic acid produced by gut Bacteroides thetaiotaomicron to promote thrombosis. [Abstract]2025 Aug 19;6(8):102260. PMID: 40749683 -
Acta Pharmacol Sin
Lethal pulmonary thromboembolism in mice induced by intravenous human umbilical cord mesenchymal stem cell-derived large extracellular vesicles in a dose- and tissue factor-dependent manner. [Abstract]2024 Nov;45(11):2300-2312. PMID: 38914677 -
Int J Biol Macromol
Discovery of a highly specific and efficacious inhibitor of human carboxylesterase 2 by large-scale screening. [Abstract]2019 Sep 15:137:261-269. PMID: 31260759 -
Int J Biol Macromol
Natural constituents of St. John's Wort inhibit the proteolytic activity of human thrombin. [Abstract]2019 Aug 1:134:622-630. PMID: 31047931 -
Blood Adv
Development of a protease-resistant ADAMTS13 to improve stability against proteolytic degradation. [Abstract]2025 Jun 10;9(11):2695-2705. PMID: 40127394 -
J Mater Chem B
2,3-Dialdehyde nanofibrillated cellulose as a potential material for the treatment of MRSA infection. [Abstract]2017 Oct 14;5(38):7876-7884. PMID: 32264389 -
RSC Adv
Inhibition of human thrombin by the constituents of licorice: inhibition kinetics and mechanistic insights through in vitro and in silico studies. [Abstract]2020 Jan 22;10(7):3626-3635. PMID: 35492646 -
Chem Biol Interact
Discovery of seven-membered ring berberine analogues as highly potent and specific hCES2A inhibitors. [Abstract]2023 Jun 1:378:110501. PMID: 37080375 -
Bioorg Chem
2019 Nov:92:103199. PMID: 31446241 -
Spectrochim Acta A Mol Biomol Spectrosc
An online, non-destructive method for simultaneously detecting chemical, biological, and physical properties of herbal injections using hyperspectral imaging with artificial intelligence. [Abstract]2022 Jan 5:264:120250. PMID: 34391991 -
Phytochemistry
Anthraquinones from Cassiae semen as thrombin inhibitors: in vitro and in silico studies. [Abstract]2019 Sep:165:112025. PMID: 31207449
Solvent & Solubility
DMSO : 100 mg/mL (156.33 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 20 mg/mL (31.27 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (275 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Tarandovskiy ID, et al. Antiplatelet agents can promote two-peaked thrombin generation in platelet rich plasma: mechanism and possible applications. PLoS One. 2013;8(2):e55688. [Content Brief]
[2]. Machlus KR, et al. Effects of tissue factor, thrombomodulin and elevated clotting factor levels on thrombin generation in the calibrated automated thrombogram. Thromb Haemost. 2009 Nov;102(5):936-44. [Content Brief]
[3]. Mothukuri GK, et al. Macrocycle synthesis strategy based on step-wise "adding and reacting" three components enables screening of large combinatorial libraries. Chem Sci. 2020 Jun 26;11(30):7858-7863. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 1.5633 mL | 7.8167 mL | 15.6333 mL | 39.0833 mL |
| 5 mM | 0.3127 mL | 1.5633 mL | 3.1267 mL | 7.8167 mL | |
| 10 mM | 0.1563 mL | 0.7817 mL | 1.5633 mL | 3.9083 mL | |
| 15 mM | 0.1042 mL | 0.5211 mL | 1.0422 mL | 2.6056 mL | |
| 20 mM | 0.0782 mL | 0.3908 mL | 0.7817 mL | 1.9542 mL | |
| 25 mM | 0.0625 mL | 0.3127 mL | 0.6253 mL | 1.5633 mL | |
| 30 mM | 0.0521 mL | 0.2606 mL | 0.5211 mL | 1.3028 mL | |
| DMSO | 40 mM | 0.0391 mL | 0.1954 mL | 0.3908 mL | 0.9771 mL |
| 50 mM | 0.0313 mL | 0.1563 mL | 0.3127 mL | 0.7817 mL | |
| 60 mM | 0.0261 mL | 0.1303 mL | 0.2606 mL | 0.6514 mL | |
| 80 mM | 0.0195 mL | 0.0977 mL | 0.1954 mL | 0.4885 mL | |
| 100 mM | 0.0156 mL | 0.0782 mL | 0.1563 mL | 0.3908 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Z-Gly-Gly-Arg-AMC acetate Related Classifications
HY-P0019A Related Classifications
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