1. Dye Reagents
  2. 7-Amino-4-methylcoumarin

7-Amino-4-methylcoumarin (Synonyms: Coumarin 120; AMC)

Cat. No.: HY-D0027 Purity: >98.0%
Handling Instructions

7-amino-4-methylcoumarin (Coumarin 120) has been used as a fluorogenic probe for many serine proteases.

For research use only. We do not sell to patients.

7-Amino-4-methylcoumarin Chemical Structure

7-Amino-4-methylcoumarin Chemical Structure

CAS No. : 26093-31-2

Size Price Stock Quantity
50 mg USD 60 In-stock
Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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Description

7-amino-4-methylcoumarin (Coumarin 120) has been used as a fluorogenic probe for many serine proteases.

Molecular Weight

175.18

Formula

C₁₀H₉NO₂

CAS No.

26093-31-2

SMILES

O=C1C=C(C)C2=CC=C(N)C=C2O1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (570.84 mM; Need ultrasonic)

H2O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 5.7084 mL 28.5421 mL 57.0841 mL
5 mM 1.1417 mL 5.7084 mL 11.4168 mL
10 mM 0.5708 mL 2.8542 mL 5.7084 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (14.27 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: 2.5 mg/mL (14.27 mM); Suspended solution; Need ultrasonic

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (14.27 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
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  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

Keywords:

7-Amino-4-methylcoumarinCoumarin 120AMCCoumarin120Coumarin-120OthersInhibitorinhibitorinhibit

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Product name:
7-Amino-4-methylcoumarin
Cat. No.:
HY-D0027
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