4-Methylumbelliferone (Synonyms: Hymecromone; 4-MU)

Name: 4-Methylumbelliferone
Brand: MedChemExpress
SKU: HY-N0187
Rating: 5.0 (7 reviews)

Cat. No.: HY-N0187 Purity: 99.62%: Data Sheet
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4-Methylumbelliferone is a hyaluronic acid biosynthesis inhibitor with antitumoral and antimetastatic effects.

For research use only. We do not sell to patients.

CAS No. : 90-33-5

Size	Stock	Quantity

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10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
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10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
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100 mg	In-stock	Estimated Time of Arrival: December 31
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Customer Review

Based on 7 publication(s) in Google Scholar

Other Forms of 4-Methylumbelliferone:

4-Methylumbelliferone sodium salt In-stock
4-Methylumbelliferone (Standard) In-stock
4-Methylumbelliferone-¹³C₄ Get quote

7 Publications Citing Use of MCE 4-Methylumbelliferone

•bioRxiv. 2024 September 07.

Gel Electrophoresis

Cell Imaging/Staining

Bio/Physico-chemical Assay

Cell Proliferation/Viability Assay

: 4-Methylumbelliferone purchased from MedChemExpress. Usage Cited in: J Transl Med. 2025 Feb 24;23(1):224. [Abstract]; Representative western blots and statistical analysis of CD68, OPN, ARG1, CD206, collagen I, and αSMA protein expression levels in ITIH5 overexpression and control co-culture systems treated with different concentrations of 4-Methylumbelliferone (0-1 mM, 24 h).

: 4-Methylumbelliferone purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2025 Aug 7;26(15):7637. [Abstract]; Electrophoresis analysis and staining of HA extracted from conditioned medium by the same cells, in the presence or absence of 4-Methylumbelliferone (4MU) and HYAL (6 U/mL). The analyzed samples include: (1) INS1E/SVF control, (2) INS1E/SVF treated with 4-Methylumbelliferone (4MU), (3) INS1E/SVF treated with HYAL (6 U/mL).

: 4-Methylumbelliferone purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2025 Aug 7;26(15):7637. [Abstract]; Phase contrast Images of INS1E pseudo-islets treated or untreated with 4-Methylumbelliferone (4MU) for 24 h.

: 4-Methylumbelliferone purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2025 Aug 7;26(15):7637. [Abstract]; Immunofluorescence analysis of INS1E cells cultured in 2D conditions, with or without 4-Methylumbelliferone (4MU) treatment, stained with anti-insulin antibody, DAPI, and Phalloidin.

: 4-Methylumbelliferone purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2025 Aug 7;26(15):7637. [Abstract]; Phase contrast images of INS1E cells grown in 2D conditions, with or without 0.250 mM 4-Methylumbelliferone (4MU) cultivated on a tissue culture plate.

: 4-Methylumbelliferone purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2025 Aug 7;26(15):7637. [Abstract]; Quantification of basal insulin secretion in INS1E cells grown in 2D and 3D conditions, with or without 0.250 mM of 4-Methylumbelliferone (4MU). Insulin levels were normalized to the total protein content (mg) of cell extracts.

: 4-Methylumbelliferone purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2025 Aug 7;26(15):7637. [Abstract]; Cell viability assessment (MTS assay) of INS1E cells treated with different concentrations of 4-Methylumbelliferone (4MU) (0.5–0.075 mM) for 48 h.

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Description

4-Methylumbelliferone is a hyaluronic acid biosynthesis inhibitor with antitumoral and antimetastatic effects.

IC₅₀ & Target

hyaluronic acid^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
A2780	IC₅₀	> 2 μM Compound: 7-hydroxy-4-methylcoumarin	Antiproliferative activity against human A2780 cells after 48 hrs by MTT assay Antiproliferative activity against human A2780 cells after 48 hrs by MTT assay	[PMID: 25350923]
A549	IC₅₀	> 100 μM Compound: Hymecromone	Cytotoxicity against human A549 cells assessed as reduction in cell viability measured after 24 hrs Cytotoxicity against human A549 cells assessed as reduction in cell viability measured after 24 hrs	[PMID: 37252094]
HEK293	IC₅₀	100 μM Compound: 4-Methylumbelliferone	Inhibition of human recombinant UGT1A1 expressed in HEK293 cells assessed as reduction in bilirubin glucuronidation by LC-MS/MS method Inhibition of human recombinant UGT1A1 expressed in HEK293 cells assessed as reduction in bilirubin glucuronidation by LC-MS/MS method	[PMID: 21030469]
HEK293	IC₅₀	100 μM Compound: 4-Methylumbelliferone	Inhibition of human recombinant UGT1A1 expressed in HEK293 cells assessed as reduction in estradiol 3-glucuronidation by LC-MS/MS method Inhibition of human recombinant UGT1A1 expressed in HEK293 cells assessed as reduction in estradiol 3-glucuronidation by LC-MS/MS method	[PMID: 21030469]
HeLa	IC₅₀	1 μM Compound: 1, 4-MU	Inhibition of human 17beta-HSD3 expressed in HeLa cells Inhibition of human 17beta-HSD3 expressed in HeLa cells	[PMID: 19954971]
HepG2	IC₅₀	> 100 μM Compound: Hymecromone	Cytotoxicity against human HepG2 cells assessed as reduction in cell viability measured after 24 hrs Cytotoxicity against human HepG2 cells assessed as reduction in cell viability measured after 24 hrs	[PMID: 37252094]
J774.A1	IC₅₀	> 100 μM Compound: Hymecromone	Cytotoxicity against mouse J774.A1 cells assessed as reduction in cell viability measured after 24 hrs Cytotoxicity against mouse J774.A1 cells assessed as reduction in cell viability measured after 24 hrs	[PMID: 37252094]
MCF7	IC₅₀	54.77 μM Compound: Hymecromone	Cytotoxicity against human MCF7 cells assessed as reduction in cell viability measured after 24 hrs Cytotoxicity against human MCF7 cells assessed as reduction in cell viability measured after 24 hrs	[PMID: 37252094]
NIH3T3	IC₅₀	23.99 μM Compound: Hymecromone	Cytotoxicity against mouse 3T3 cells assessed as reduction in cell viability measured after 24 hrs Cytotoxicity against mouse 3T3 cells assessed as reduction in cell viability measured after 24 hrs	[PMID: 37252094]
SK-MEL-28	IC₅₀	0.58 μM Compound: 6	Anticancer activity against human SK-MEL-28 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay Anticancer activity against human SK-MEL-28 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay	[PMID: 37197457]
SK-MEL3	IC₅₀	1.6 μM Compound: 6	Anticancer activity against human SK-MEL3 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay Anticancer activity against human SK-MEL3 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay	[PMID: 37197457]
SMMC-7721	IC₅₀	356 mM Compound: 14.1	Antiproliferative activity against human SMMC-7721 cells incubated for 48 hrs by MTT assay Antiproliferative activity against human SMMC-7721 cells incubated for 48 hrs by MTT assay	[PMID: 38340509]
SiHa	IC₅₀	> 100 μM Compound: Hymecromone	Cytotoxicity against human SiHa cells assessed as reduction in cell viability measured after 24 hrs Cytotoxicity against human SiHa cells assessed as reduction in cell viability measured after 24 hrs	[PMID: 37252094]
U-937	CC₅₀	> 2000 μM Compound: 10	Cytotoxicity against human U937 cells after 48 hrs by trypan blue assay Cytotoxicity against human U937 cells after 48 hrs by trypan blue assay	[PMID: 22925447]
U-937	CC₅₀	> 2000 μM Compound: 12	Cytotoxicity against human U937 cells by trypan blue assay after 48 hrs Cytotoxicity against human U937 cells by trypan blue assay after 48 hrs	[PMID: 18060791]
U-937	CC₅₀	> 2000 μM Compound: 7	Cytotoxicity against human U937 cells by trypan blue assay after 48 hrs Cytotoxicity against human U937 cells by trypan blue assay after 48 hrs	[PMID: 18060791]
U-937	IC₅₀	710.9 μM Compound: 10	Antiproliferative activity against human U937 cells assessed as incorporation of [3H]-methyl-thymidine after 12 hrs by scintillation counting Antiproliferative activity against human U937 cells assessed as incorporation of [3H]-methyl-thymidine after 12 hrs by scintillation counting	[PMID: 22925447]
U-937	IC₅₀	710.9 μM Compound: 7	Growth inhibition of human U937 cells by [3H]thymidine incorporation assay Growth inhibition of human U937 cells by [3H]thymidine incorporation assay	[PMID: 18060791]
U-937	IC₅₀	> 2000 μM Compound: 12	Growth inhibition of human U937 cells by [3H]thymidine incorporation assay Growth inhibition of human U937 cells by [3H]thymidine incorporation assay	[PMID: 18060791]

In Vitro

4-Methylumbelliferone (4-MU) affects several key steps of angiogenesis, including endothelial cell proliferation, adhesion, tube formation, and extracellular matrix remodeling. Half-maximal inhibitory concentrations (IC₅₀) values in the proliferation assay are 0.65±0.04 and 0.37±0.03 mM for human microvascular endothelial cells (HMEC) and RF-24 endothelial cells, respectively. 4-Methylumbelliferone (2 mM) treatment for 24 h induces apoptosis in 13% of HMEC and 5% of RF-24 cells. The number of adherent endothelial cells decreases by >20% after 24 h of treatment with 1 mM 4-Methylumbelliferone. Minimal inhibitory concentrations in the tube formation assay are 2 and 0.5 mM 4-Methylumbelliferone for HMEC and RF-24, respectively. Matrix metalloproteinase-2 expression is differentially altered upon 4-Methylumbelliferone treatment in both tested endothelial cell lines^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

176.17

Formula

C₁₀H₈O₃

CAS No.

90-33-5

Appearance

Solid

Color

White to off-white

SMILES

O=C1C=C(C)C2=CC=C(O)C=C2O1

Structure Classification

Initial Source

Microorganisms

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	1 year
	-20°C	6 months

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (567.63 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	5.6763 mL	28.3817 mL	56.7634 mL
5 mM	1.1353 mL	5.6763 mL	11.3527 mL
10 mM	0.5676 mL	2.8382 mL	5.6763 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (14.19 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (14.19 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (14.19 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 50% PEG300 50% Saline
Solubility: 50 mg/mL (283.82 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.62%

Select Batch:

Data Sheet (273 KB) SDS (393 KB)

COA (247 KB) HNMR (117 KB) RP-HPLC (259 KB)

Handling Instructions (2659 KB)

References

[1]. García-Vilas JA, et al. 4-methylumbelliferone inhibits angiogenesis in vitro and in vivo. J Agric Food Chem. 2013 May 1;61(17):4063-71. [Content Brief]

Cell Assay
^[1]

The MTT dye reduction assay in 96-well microplates is used. The assay is dependent on the reduction of MTT by mitochondrial dehydrogenases of viable cells to a blue formazan product, which can be measured spectrophotometrically. Endothelial cells (2.5×10³ cells in a total volume of 100 μL of complete medium) are incubated in each well with serial dilutions of 4-Methylumbelliferone (4-MU) (0, 0.5, 1, 1.5 and 2 mM). After 3 days of incubation in the dark (37°C, 5% CO₂ in a humid atmosphere), 10 μL of MTT (5 mg/mL in PBS) is added to each well, and the plate is incubated for further a 4 h (37°C). The formazan is dissolved in 150 μL of 0.04 N HCl-2 propanol, and samples are spectrophotometrically measured at 550 nm. All determinations are carried out in quadruplicate, and at least three independent experiments are carried out. IC₅₀ values are calculated as those concentrations of compound yielding 50% cell survival, taking the values obtained for control as 100%^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

[1]. García-Vilas JA, et al. 4-methylumbelliferone inhibits angiogenesis in vitro and in vivo. J Agric Food Chem. 2013 May 1;61(17):4063-71. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	5.6763 mL	28.3817 mL	56.7634 mL	141.9084 mL
	5 mM	1.1353 mL	5.6763 mL	11.3527 mL	28.3817 mL
	10 mM	0.5676 mL	2.8382 mL	5.6763 mL	14.1908 mL
	15 mM	0.3784 mL	1.8921 mL	3.7842 mL	9.4606 mL
	20 mM	0.2838 mL	1.4191 mL	2.8382 mL	7.0954 mL
	25 mM	0.2271 mL	1.1353 mL	2.2705 mL	5.6763 mL
	30 mM	0.1892 mL	0.9461 mL	1.8921 mL	4.7303 mL
	40 mM	0.1419 mL	0.7095 mL	1.4191 mL	3.5477 mL
	50 mM	0.1135 mL	0.5676 mL	1.1353 mL	2.8382 mL
	60 mM	0.0946 mL	0.4730 mL	0.9461 mL	2.3651 mL
	80 mM	0.0710 mL	0.3548 mL	0.7095 mL	1.7739 mL
	100 mM	0.0568 mL	0.2838 mL	0.5676 mL	1.4191 mL

4-Methylumbelliferone Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

4-Methylumbelliferone90-33-5Hymecromone 4-MUOthersInhibitorinhibitorinhibit

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4-Methylumbelliferone (Synonyms: Hymecromone; 4-MU)

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4-Methylumbelliferone Related Antibodies

7 Publications Citing Use of MCE 4-Methylumbelliferone

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4-Methylumbelliferone Related Antibodies

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Complete Stock Solution Preparation Table

4-Methylumbelliferone Related Classifications

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