Cat. No.: HY-N0187

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4-Methylumbelliferone

(Synonyms: Hymecromone; 4-MU)

8 Cited Publications

Customer Review

Based on 8 publication(s) in Google Scholar

4-Methylumbelliferone is a hyaluronic acid biosynthesis inhibitor with antitumoral and antimetastatic effects.

For research use only. We do not sell to patients.

Purity: 99.62%
CAS No.: 90-33-5
Formula: C₁₀H₈O₃
Molecular Weight:176.17

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 1 year , -20°C, 6 months

4-Methylumbelliferone Chemical Structure

CAS No.: 90-33-5

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Publications (8)
Solvent & Solubility
- In Vitro
- In Vivo
Protocol
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

4-Methylumbelliferone is a hyaluronic acid biosynthesis inhibitor with antitumoral and antimetastatic effects.

IC₅₀ & Target

hyaluronic acid^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
A2780	IC₅₀	>2 μM Compound: 7-hydroxy-4-methylcoumarin	Antiproliferative activity against human A2780 cells after 48 hrs by MTT assay Antiproliferative activity against human A2780 cells after 48 hrs by MTT assay	[PMID: 25350923]
A549	IC₅₀	>100 μM Compound: Hymecromone	Cytotoxicity against human A549 cells assessed as reduction in cell viability measured after 24 hrs Cytotoxicity against human A549 cells assessed as reduction in cell viability measured after 24 hrs	[PMID: 37252094]
HEK293	IC₅₀	100 μM Compound: 4-Methylumbelliferone	Inhibition of human recombinant UGT1A1 expressed in HEK293 cells assessed as reduction in bilirubin glucuronidation by LC-MS/MS method Inhibition of human recombinant UGT1A1 expressed in HEK293 cells assessed as reduction in bilirubin glucuronidation by LC-MS/MS method	[PMID: 21030469]
HEK293	IC₅₀	100 μM Compound: 4-Methylumbelliferone	Inhibition of human recombinant UGT1A1 expressed in HEK293 cells assessed as reduction in estradiol 3-glucuronidation by LC-MS/MS method Inhibition of human recombinant UGT1A1 expressed in HEK293 cells assessed as reduction in estradiol 3-glucuronidation by LC-MS/MS method	[PMID: 21030469]
HeLa	IC₅₀	1 μM Compound: 1, 4-MU	Inhibition of human 17beta-HSD3 expressed in HeLa cells Inhibition of human 17beta-HSD3 expressed in HeLa cells	[PMID: 19954971]
HepG2	IC₅₀	>100 μM Compound: Hymecromone	Cytotoxicity against human HepG2 cells assessed as reduction in cell viability measured after 24 hrs Cytotoxicity against human HepG2 cells assessed as reduction in cell viability measured after 24 hrs	[PMID: 37252094]
J774.A1	IC₅₀	>100 μM Compound: Hymecromone	Cytotoxicity against mouse J774.A1 cells assessed as reduction in cell viability measured after 24 hrs Cytotoxicity against mouse J774.A1 cells assessed as reduction in cell viability measured after 24 hrs	[PMID: 37252094]
MCF7	IC₅₀	54.77 μM Compound: Hymecromone	Cytotoxicity against human MCF7 cells assessed as reduction in cell viability measured after 24 hrs Cytotoxicity against human MCF7 cells assessed as reduction in cell viability measured after 24 hrs	[PMID: 37252094]
NIH3T3	IC₅₀	23.99 μM Compound: Hymecromone	Cytotoxicity against mouse 3T3 cells assessed as reduction in cell viability measured after 24 hrs Cytotoxicity against mouse 3T3 cells assessed as reduction in cell viability measured after 24 hrs	[PMID: 37252094]
SiHa	IC₅₀	>100 μM Compound: Hymecromone	Cytotoxicity against human SiHa cells assessed as reduction in cell viability measured after 24 hrs Cytotoxicity against human SiHa cells assessed as reduction in cell viability measured after 24 hrs	[PMID: 37252094]
SK-MEL-28	IC₅₀	0.58 μM Compound: 6	Anticancer activity against human SK-MEL-28 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay Anticancer activity against human SK-MEL-28 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay	[PMID: 37197457]
SK-MEL3	IC₅₀	1.6 μM Compound: 6	Anticancer activity against human SK-MEL3 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay Anticancer activity against human SK-MEL3 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay	[PMID: 37197457]
SMMC-7721	IC₅₀	356 mM Compound: 14.1	Antiproliferative activity against human SMMC-7721 cells incubated for 48 hrs by MTT assay Antiproliferative activity against human SMMC-7721 cells incubated for 48 hrs by MTT assay	[PMID: 38340509]
U-937	CC₅₀	>2000 μM Compound: 12	Cytotoxicity against human U937 cells by trypan blue assay after 48 hrs Cytotoxicity against human U937 cells by trypan blue assay after 48 hrs	[PMID: 18060791]
U-937	IC₅₀	710.9 μM Compound: 10	Antiproliferative activity against human U937 cells assessed as incorporation of [3H]-methyl-thymidine after 12 hrs by scintillation counting Antiproliferative activity against human U937 cells assessed as incorporation of [3H]-methyl-thymidine after 12 hrs by scintillation counting	[PMID: 22925447]
U-937	CC₅₀	>2000 μM Compound: 10	Cytotoxicity against human U937 cells after 48 hrs by trypan blue assay Cytotoxicity against human U937 cells after 48 hrs by trypan blue assay	[PMID: 22925447]

In Vitro

4-Methylumbelliferone (4-MU) affects several key steps of angiogenesis, including endothelial cell proliferation, adhesion, tube formation, and extracellular matrix remodeling. Half-maximal inhibitory concentrations (IC₅₀) values in the proliferation assay are 0.65±0.04 and 0.37±0.03 mM for human microvascular endothelial cells (HMEC) and RF-24 endothelial cells, respectively. 4-Methylumbelliferone (2 mM) treatment for 24 h induces apoptosis in 13% of HMEC and 5% of RF-24 cells. The number of adherent endothelial cells decreases by >20% after 24 h of treatment with 1 mM 4-Methylumbelliferone. Minimal inhibitory concentrations in the tube formation assay are 2 and 0.5 mM 4-Methylumbelliferone for HMEC and RF-24, respectively. Matrix metalloproteinase-2 expression is differentially altered upon 4-Methylumbelliferone treatment in both tested endothelial cell lines^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 90-33-5
Appearance Solid
Molecular Weight 176.17
Formula C₁₀H₈O₃
Color White to off-white
SMILES

O=C1C=C(C)C2=CC=C(O)C=C2O1
Synonyms

Hymecromone; 4-MU
Structure Classification
- Phenylpropanoids
- Coumarins
- Phenols
- Monophenols
Initial Source
- Microorganisms
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	1 year
	-20°C	6 months

Publications (8)

All WBGel ElectrophoresisCell Imaging/StainingIFBio/Physico-chemical AssayCell Proliferation/Viability Assay

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Journal Impact Factor

J Transl Med

ITIH5-mediated fibroblast/macrophage crosstalk exacerbates cardiac remodelling after myocardial infarction. [Abstract]

2025 Feb 24;23(1):224. PMID: 39994656

4-Methylumbelliferone purchased from MedChemExpress. Usage Cited in: J Transl Med. 2025 Feb 24;23(1):224. [Abstract]

Representative western blots and statistical analysis of CD68, OPN, ARG1, CD206, collagen I, and αSMA protein expression levels in ITIH5 overexpression and control co-culture systems treated with different concentrations of 4-Methylumbelliferone (0-1 mM, 24 h).
Int J Biol Macromol

Compacting hyaluronan into nanogels induces an enhanced macropinocytosis in MC38 cells. [Abstract]

2025 Apr 28;310(Pt 4):143599. PMID: 40306505
Int J Biol Macromol

HIF1α lactylation enhances KIAA1199 transcription to promote angiogenesis and vasculogenic mimicry in prostate cancer. [Abstract]

2022 Dec 1;222(Pt B):2225-2243. PMID: 36209908
Cell Rep

UDP-glucose dehydrogenase supports autophagy-deficient PDAC growth via increasing hyaluronic acid biosynthesis. [Abstract]

2024 Feb 16;43(2):113808. PMID: 38367236
Commun Biol

Targeting HAS2 to enhance anti-tumor immunity in pancreatic cancer via PD-L1 regulation. [Abstract]

2026 Apr 8;9(1):764. PMID: 41951812
Int J Mol Sci

Dual Effect of 4-Methylumbelliferone on INS1E Cells: Enhancing Migration and Glucose-Stimulated Insulin Secretion. [Abstract]

2025 Aug 7;26(15):7637. PMID: 40806765

4-Methylumbelliferone purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2025 Aug 7;26(15):7637. [Abstract]

Electrophoresis analysis and staining of HA extracted from conditioned medium by the same cells, in the presence or absence of 4-Methylumbelliferone (4MU) and HYAL (6 U/mL). The analyzed samples include: (1) INS1E/SVF control, (2) INS1E/SVF treated with 4-Methylumbelliferone (4MU), (3) INS1E/SVF treated with HYAL (6 U/mL).

4-Methylumbelliferone purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2025 Aug 7;26(15):7637. [Abstract]

Phase contrast Images of INS1E pseudo-islets treated or untreated with 4-Methylumbelliferone (4MU) for 24 h.

4-Methylumbelliferone purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2025 Aug 7;26(15):7637. [Abstract]

Immunofluorescence analysis of INS1E cells cultured in 2D conditions, with or without 4-Methylumbelliferone (4MU) treatment, stained with anti-insulin antibody, DAPI, and Phalloidin.

4-Methylumbelliferone purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2025 Aug 7;26(15):7637. [Abstract]

Phase contrast images of INS1E cells grown in 2D conditions, with or without 0.250 mM 4-Methylumbelliferone (4MU) cultivated on a tissue culture plate.

4-Methylumbelliferone purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2025 Aug 7;26(15):7637. [Abstract]

Quantification of basal insulin secretion in INS1E cells grown in 2D and 3D conditions, with or without 0.250 mM of 4-Methylumbelliferone (4MU). Insulin levels were normalized to the total protein content (mg) of cell extracts.

4-Methylumbelliferone purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2025 Aug 7;26(15):7637. [Abstract]

Cell viability assessment (MTS assay) of INS1E cells treated with different concentrations of 4-Methylumbelliferone (4MU) (0.5–0.075 mM) for 48 h.
Pancreatology

Expression of versican isoforms V0/V1 by pancreatic cancer associated fibroblasts increases fibroblast proliferation. [Abstract]

2024 Aug;24(5):719-731. PMID: 38719756
bioRxiv

2024 September 07.

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (567.63 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

5.6763 mL

28.3817 mL

56.7634 mL

5 mM

1.1353 mL

5.6763 mL

11.3527 mL

10 mM

0.5676 mL

2.8382 mL

5.6763 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (14.19 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (14.19 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (14.19 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 50% PEG300 50% Saline

Solubility: 50 mg/mL (283.82 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Protocol

Cell Assay^[1]

The MTT dye reduction assay in 96-well microplates is used. The assay is dependent on the reduction of MTT by mitochondrial dehydrogenases of viable cells to a blue formazan product, which can be measured spectrophotometrically. Endothelial cells (2.5×10³ cells in a total volume of 100 μL of complete medium) are incubated in each well with serial dilutions of 4-Methylumbelliferone (4-MU) (0, 0.5, 1, 1.5 and 2 mM). After 3 days of incubation in the dark (37°C, 5% CO₂ in a humid atmosphere), 10 μL of MTT (5 mg/mL in PBS) is added to each well, and the plate is incubated for further a 4 h (37°C). The formazan is dissolved in 150 μL of 0.04 N HCl-2 propanol, and samples are spectrophotometrically measured at 550 nm. All determinations are carried out in quadruplicate, and at least three independent experiments are carried out. IC₅₀ values are calculated as those concentrations of compound yielding 50% cell survival, taking the values obtained for control as 100%^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (273 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. García-Vilas JA, et al. 4-methylumbelliferone inhibits angiogenesis in vitro and in vivo. J Agric Food Chem. 2013 May 1;61(17):4063-71. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	5.6763 mL	28.3817 mL	56.7634 mL	141.9084 mL
	5 mM	1.1353 mL	5.6763 mL	11.3527 mL	28.3817 mL
	10 mM	0.5676 mL	2.8382 mL	5.6763 mL	14.1908 mL
	15 mM	0.3784 mL	1.8921 mL	3.7842 mL	9.4606 mL
	20 mM	0.2838 mL	1.4191 mL	2.8382 mL	7.0954 mL
	25 mM	0.2271 mL	1.1353 mL	2.2705 mL	5.6763 mL
	30 mM	0.1892 mL	0.9461 mL	1.8921 mL	4.7303 mL
	40 mM	0.1419 mL	0.7095 mL	1.4191 mL	3.5477 mL
	50 mM	0.1135 mL	0.5676 mL	1.1353 mL	2.8382 mL
	60 mM	0.0946 mL	0.4730 mL	0.9461 mL	2.3651 mL
	80 mM	0.0710 mL	0.3548 mL	0.7095 mL	1.7739 mL
	100 mM	0.0568 mL	0.2838 mL	0.5676 mL	1.4191 mL

Keywords

4-Methylumbelliferone

8 Cited Publications

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4-Methylumbelliferone

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Publications Citing Use of MedChemExpress (MCE) 4-Methylumbelliferone

Biological Activity

Chemical Information

Publications (8)

Solvent & Solubility

Protocol

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

4-Methylumbelliferone Related Classifications

HY-N0187 Related Classifications