Gly-Pro-AMC hydrobromide
Based on 1 publication(s) in Google Scholar
Gly-Pro-AMC hydrobromide is a fluorescent dye, it can be used as a specific fluorescent substrate for detecting Dipeptidyl peptidase IV (DPP-IV) activity.
For research use only. We do not sell to patients.
- Purity: 99.81%
- CAS No.: 115035-46-6
- Formula: C17H20BrN3O4
- Molecular Weight:410.27
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Storage:
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) Gly-Pro-AMC hydrobromide
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Biological Activity
Gly-Pro-AMC hydrobromide (50 μM; 20 min; 37 °C) can be used as a specific fluorescent substrate for detecting DPP-IV activity[1]. Gly-Pro-AMC hydrobromide have fluorescence properties, with the fluorescence detection conditions: excitation wavelength 350 nm, fluorescence emission spectrum detection 450 nm[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 115035-46-6
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Appearance Solid
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Molecular Weight 410.27
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Formula C17H20BrN3O4
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Color Off-white to light yellow
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SMILES
O=C(NC1=CC=C(C(O2)=C1)C(C)=CC2=O)[C@H]3N(C(CN)=O)CCC3.[H]Br
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (1)
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Journal Impact Factor
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Most Recent
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Curr Res Food Sci
Discovery of novel dipeptidyl peptidase-IV inhibitory peptides derived from walnut protein and their bioactivities in vivo and in vitro. [Abstract]2024 Oct 24:9:100893. PMID: 39555024
Solvent & Solubility
H2O : 100 mg/mL (243.74 mM; Need ultrasonic)
DMSO : 100 mg/mL (243.74 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.09 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.09 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Purity & Documentation
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Data Sheet (273 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Lammi C, et al. Soybean- and Lupin-Derived Peptides Inhibit DPP-IV Activity on In Situ Human Intestinal Caco-2 Cells and Ex Vivo Human Serum. Nutrients. 2018 Aug 13;10(8):1082. [Content Brief]
[2]. Gallego M, et al. Dipeptidyl peptidase IV inhibitory peptides generated in Spanish dry-cured ham. Meat Sci. 2014 Feb;96(2 Pt A):757-61. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 2.4374 mL | 12.1871 mL | 24.3742 mL | 60.9355 mL |
| 5 mM | 0.4875 mL | 2.4374 mL | 4.8748 mL | 12.1871 mL | |
| 10 mM | 0.2437 mL | 1.2187 mL | 2.4374 mL | 6.0935 mL | |
| 15 mM | 0.1625 mL | 0.8125 mL | 1.6249 mL | 4.0624 mL | |
| 20 mM | 0.1219 mL | 0.6094 mL | 1.2187 mL | 3.0468 mL | |
| 25 mM | 0.0975 mL | 0.4875 mL | 0.9750 mL | 2.4374 mL | |
| 30 mM | 0.0812 mL | 0.4062 mL | 0.8125 mL | 2.0312 mL | |
| 40 mM | 0.0609 mL | 0.3047 mL | 0.6094 mL | 1.5234 mL | |
| 50 mM | 0.0487 mL | 0.2437 mL | 0.4875 mL | 1.2187 mL | |
| 60 mM | 0.0406 mL | 0.2031 mL | 0.4062 mL | 1.0156 mL | |
| 80 mM | 0.0305 mL | 0.1523 mL | 0.3047 mL | 0.7617 mL | |
| 100 mM | 0.0244 mL | 0.1219 mL | 0.2437 mL | 0.6094 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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