Gly-Pro-AMC hydrobromide

Name: Gly-Pro-AMC hydrobromide
Brand: MedChemExpress
SKU: HY-137834
Rating: 4.5 (1 reviews)

Cat. No.: HY-137834 Purity: 98.68%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Gly-Pro-AMC hydrobromide is a fluorescent dye, it can be used as a specific fluorescent substrate for detecting Dipeptidyl peptidase IV (DPP-IV) activity.

For research use only. We do not sell to patients.

Gly-Pro-AMC hydrobromide Chemical Structure

CAS No. : 115035-46-6

Size	Price	Stock	Quantity
Solid + Solvent
10 mM * 1 mL in DMSO ready for reconstitution	USD 93	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 93	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 85	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 150	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 350	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 550	In-stock	Estimated Time of Arrival: December 31
100 mg		Get quote
200 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Gly-Pro-AMC hydrobromide:

Gly-Pro-AMC Get quote

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Gly-Pro-AMC hydrobromide is a fluorescent dye, it can be used as a specific fluorescent substrate for detecting Dipeptidyl peptidase IV (DPP-IV) activity^[1]^[2].

In Vitro

Gly-Pro-AMC hydrobromide (50 µM; 20 min; 37 °C) can be used as a specific fluorescent substrate for detecting DPP-IV activity^[1].
Gly-Pro-AMC hydrobromide have fluorescence properties, with the fluorescence detection conditions: excitation wavelength 350 nm, fluorescence emission spectrum detection 450 nm^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

410.26

Formula

C₁₇H₂₀BrN₃O₄

CAS No.

115035-46-6

Appearance

Solid

Color

Off-white to light yellow

SMILES

O=C(NC1=CC=C(C(O2)=C1)C(C)=CC2=O)[C@H]3N(C(CN)=O)CCC3.[H]Br

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (243.75 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 100 mg/mL (243.75 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.4375 mL	12.1874 mL	24.3748 mL
5 mM	0.4875 mL	2.4375 mL	4.8750 mL
10 mM	0.2437 mL	1.2187 mL	2.4375 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.09 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.09 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (6.09 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 98.68%

Select Batch:

Data Sheet (272 KB) SDS (0 KB)

COA (248 KB) RP-HPLC (108 KB) MS (195 KB)

Handling Instructions (2659 KB)

References

[1]. Lammi C, et al. Soybean- and Lupin-Derived Peptides Inhibit DPP-IV Activity on In Situ Human Intestinal Caco-2 Cells and Ex Vivo Human Serum. Nutrients. 2018 Aug 13;10(8):1082. [Content Brief]

[2]. Gallego M, et al. Dipeptidyl peptidase IV inhibitory peptides generated in Spanish dry-cured ham. Meat Sci. 2014 Feb;96(2 Pt A):757-61. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	2.4375 mL	12.1874 mL	24.3748 mL	60.9370 mL
	5 mM	0.4875 mL	2.4375 mL	4.8750 mL	12.1874 mL
	10 mM	0.2437 mL	1.2187 mL	2.4375 mL	6.0937 mL
	15 mM	0.1625 mL	0.8125 mL	1.6250 mL	4.0625 mL
	20 mM	0.1219 mL	0.6094 mL	1.2187 mL	3.0468 mL
	25 mM	0.0975 mL	0.4875 mL	0.9750 mL	2.4375 mL
	30 mM	0.0812 mL	0.4062 mL	0.8125 mL	2.0312 mL
	40 mM	0.0609 mL	0.3047 mL	0.6094 mL	1.5234 mL
	50 mM	0.0487 mL	0.2437 mL	0.4875 mL	1.2187 mL
	60 mM	0.0406 mL	0.2031 mL	0.4062 mL	1.0156 mL
	80 mM	0.0305 mL	0.1523 mL	0.3047 mL	0.7617 mL
	100 mM	0.0244 mL	0.1219 mL	0.2437 mL	0.6094 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.