2. Metabolic Enzyme/Protease
  3. Phosphatase
  4. 4-Methylumbelliferyl phosphate

4-Methylumbelliferyl phosphate  (Synonyms: 4-MUP; MUP)

Cat. No.: HY-D0994 Purity: 99.84%
COA
SDS
Handling Instructions Technical Support

4-Methylumbelliferyl phosphate (4-MUP), an anionic organophosphate, is a acid and alkaline phosphatase fluorogenic substrate. 4-Methylumbelliferyl phosphate is also a nerve agent simulant.

For research use only. We do not sell to patients.

4-Methylumbelliferyl phosphate

4-Methylumbelliferyl phosphate Chemical Structure

CAS No. : 3368-04-5

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in Water In-stock
Solid
100 mg In-stock
500 mg In-stock
1 g In-stock
5 g   Get quote  
10 g   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of 4-Methylumbelliferyl phosphate:

4-Methylumbelliferyl phosphate Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE 4-Methylumbelliferyl phosphate

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

4-Methylumbelliferyl phosphate (4-MUP), an anionic organophosphate, is a acid and alkaline phosphatase fluorogenic substrate. 4-Methylumbelliferyl phosphate is also a nerve agent simulant[1][2][3].

In Vitro

Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
(1) Substrate Preparation: Prepare 4-MUP at a 0.6 mM to 1 mg/mL concentration in a buffer with an alkaline pH (e.g., Tris-NaCl pH 10, or Diethanolamine buffer).
(2) Sample Preparation: Use samples (e.g., lysates, supernatant) diluted in appropriate buffers.
(3) Incubation: Mix 1 part sample with 1-2 parts 4-MUP substrate solution.
(4) Reaction Conditions: Incubate at 37°C or room temperature for 30–60 minutes, protected from light.
(5) Detection: Measure fluorescence at excitation ~ 360 nm and emission ~ 440 nm.
(6) Stopping the Reaction: The reaction can be stopped by adding high pH buffers, such as 0.2 M Glycine/NaOH.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

4-Methylumbelliferyl phosphate Related Antibodies
Molecular Weight

256.15

Formula

C10H9O6P
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C1C=C(C)C2=CC=C(OP(O)(O)=O)C=C2O1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

H2O : 17.5 mg/mL (68.32 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.9040 mL 19.5198 mL 39.0396 mL
5 mM 0.7808 mL 3.9040 mL 7.8079 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.84%

SDS (393 KB)

COA (248 KB) HNMR (252 KB) CNMR (246 KB) LCMS (95 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 3.9040 mL 19.5198 mL 39.0396 mL 97.5991 mL
5 mM 0.7808 mL 3.9040 mL 7.8079 mL 19.5198 mL
10 mM 0.3904 mL 1.9520 mL 3.9040 mL 9.7599 mL
15 mM 0.2603 mL 1.3013 mL 2.6026 mL 6.5066 mL
20 mM 0.1952 mL 0.9760 mL 1.9520 mL 4.8800 mL
25 mM 0.1562 mL 0.7808 mL 1.5616 mL 3.9040 mL
30 mM 0.1301 mL 0.6507 mL 1.3013 mL 3.2533 mL
40 mM 0.0976 mL 0.4880 mL 0.9760 mL 2.4400 mL
50 mM 0.0781 mL 0.3904 mL 0.7808 mL 1.9520 mL
60 mM 0.0651 mL 0.3253 mL 0.6507 mL 1.6267 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

4-Methylumbelliferyl phosphate Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

4-Methylumbelliferyl3368-04-54-MUP MUPPhosphataseAlkalinephosphatasesfluorogenicsubstratekineticInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Requested Quantity *

Applicant Name *

 

Salutation

Email Address *

 

Phone Number *

Department

 

Organization Name *

City

State

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
4-Methylumbelliferyl phosphate
Cat. No.:
HY-D0994
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

English - EN (393 KB) Français - FR (393 KB) Deutsch - DE (393 KB) Norwegian - NO (393 KB) Español - ES (393 KB) Swedish - SV (393 KB) Italian - IT (393 KB) Korean - KR (393 KB) Portuguese - PT (393 KB)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.