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  2. 4-Methylumbelliferyl phosphate

4-Methylumbelliferyl phosphate (Synonyms: 4-MUP; MUP)

Cat. No.: HY-D0994 Purity: 99.93%
Handling Instructions

4-Methylumbelliferyl phosphate (4-MUP) is a fluorogenic substrate of alkaline phosphatases.

For research use only. We do not sell to patients.

4-Methylumbelliferyl phosphate Chemical Structure

4-Methylumbelliferyl phosphate Chemical Structure

CAS No. : 3368-04-5

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10 mM * 1 mL in Water USD 55 In-stock
Estimated Time of Arrival: December 31
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Estimated Time of Arrival: December 31
100 mg USD 50 In-stock
Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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4-Methylumbelliferyl phosphate (4-MUP) is a fluorogenic substrate of alkaline phosphatases[1].

In Vitro

4-Methylumbelliferyl phosphate monoester previously described as one of a series of 4-methylumbelliferone conjugates is later found to be the PlP2-pyrophosphate diester and is not hydrolysed by serum phosphomonoesterases . 4-Methylumbelliferyl phosphate has a significant advantage over currently available substrates in that its useful concentration range extends to about 0.1 μM[1].
If the concentration of 4-Methylumbelliferyl phosphate is held constant and the pH is varied, the activity of the enzyme passes through a maximum[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight







Room temperature in continental US; may vary elsewhere.


-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

H2O : 20.83 mg/mL (81.32 mM; Need ultrasonic)

Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.9040 mL 19.5198 mL 39.0396 mL
5 mM 0.7808 mL 3.9040 mL 7.8079 mL
10 mM 0.3904 mL 1.9520 mL 3.9040 mL
*Please refer to the solubility information to select the appropriate solvent.
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This equation is commonly abbreviated as: C1V1 = C2V2

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4-Methylumbelliferyl phosphate
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