The analysis of alkaline phosphatase isoenzyme using 4-methylumbelliferyl phosphate as substrate on a cellulose acetate membrane

  • Clin Chim Acta. 1979 Feb 1;91(3):273-6. doi: 10.1016/0009-8981(79)90483-2.
F Watanabe ,  M Takano ,  F Tanaka ,  N Amino ,  C Hayashi ,  K Miyai
PMID: 761403 DOI: 10.1016/0009-8981(79)90483-2
Abstract

A new procedure is established for the analysis of Alkaline Phosphatase isoenzymes. The electrophoretic separation on cellulose acetate membrane coupled with the detection of Alkaline Phosphatase activity with 4-methyl-umbelliferyl phosphate as a substrate is described. The proposed method would be useful for the analysis of sample of micro-scale quantities and low activities.

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