4-Methylumbelliferyl phosphate disodium  (Synonyms: 4-MUP disodium; MUP disodium)

4-Methylumbelliferyl phosphate (4-MUP) disodium, an anionic organophosphate, is a acid and alkaline phosphatase fluorogenic substrate. 4-Methylumbelliferyl phosphate disodium is also a nerve agent simulant^[1][2][3].

Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
(1) Substrate Preparation: Prepare 4-MUP at a 0.6 mM to 1 mg/mL concentration in a buffer with an alkaline pH (e.g., Tris-NaCl pH 10, or Diethanolamine buffer).
(2) Sample Preparation: Use samples (e.g., lysates, supernatant) diluted in appropriate buffers.
(3) Incubation: Mix 1 part sample with 1-2 parts 4-MUP substrate solution.
(4) Reaction Conditions: Incubate at 37°C or room temperature for 30–60 minutes, protected from light.
(5) Detection: Measure fluorescence at excitation ~ 360 nm and emission ~ 440 nm.
(6) Stopping the Reaction: The reaction can be stopped by adding high pH buffers, such as 0.2 M Glycine/NaOH.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

300.11

C₁₀H₇Na₂O₆P

22919-26-2

460

344

O=C1C=C(C)C2=CC=C(OP(O[Na])(O[Na])=O)C=C2O1

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Chambers JP, et al. Determination of serum acid phosphatase in Gaucher's disease using 4-methylumbelliferyl phosphate. Clin Chim Acta. 1977 Oct 1;80(1):67-77.  [Content Brief]

  [2]. Watanabe F, et al. The analysis of alkaline phosphatase isoenzyme using 4-methylumbelliferyl phosphate as substrate on a cellulose acetate membrane. Clin Chim Acta. 1979 Feb 1;91(3):273-6.  [Content Brief]

  [3]. Hao Chen, et al. Autonomic Molecular Transport for Ultrasensitive Surface-Enhanced Infrared Absorption Spectroscopy. ACS Appl. Polym. Mater. 2020, 2, 9, 3929-3935