4-Methylumbelliferyl phosphate disodium
Based on 1 publication(s) in Google Scholar
4-Methylumbelliferyl phosphate (4-MUP) disodium, an anionic organophosphate, is a acid and alkaline phosphatase fluorogenic substrate. 4-Methylumbelliferyl phosphate disodium is also a nerve agent simulant.
For research use only. We do not sell to patients.
- CAS No.: 22919-26-2
- Formula: C10H7Na2O6P
- Molecular Weight:300.11
-
Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
- Purity & Documentation
- References
-
Help & FAQs
Publications Citing Use of MedChemExpress (MCE) 4-Methylumbelliferyl phosphate disodium
More
Biological Activity
Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
(1) Substrate Preparation: Prepare 4-MUP at a 0.6 mM to 1 mg/mL concentration in a buffer with an alkaline pH (e.g., Tris-NaCl pH 10, or Diethanolamine buffer).
(2) Sample Preparation: Use samples (e.g., lysates, supernatant) diluted in appropriate buffers.
(3) Incubation: Mix 1 part sample with 1-2 parts 4-MUP substrate solution.
(4) Reaction Conditions: Incubate at 37°C or room temperature for 30–60 minutes, protected from light.
(5) Detection: Measure fluorescence at excitation ~ 360 nm and emission ~ 440 nm.
(6) Stopping the Reaction: The reaction can be stopped by adding high pH buffers, such as 0.2 M Glycine/NaOH.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
460
344
Chemical Information
-
CAS No. 22919-26-2
-
Molecular Weight 300.11
-
Formula C10H7Na2O6P
-
SMILES
O=C1C=C(C)C2=CC=C(OP(O[Na])(O[Na])=O)C=C2O1
-
Synonyms
4-MUP disodium; MUP disodium
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications (1)
-
Journal Impact Factor
-
Most Recent
-
Research (Wash D C)
A Noninvasive Menstrual Blood-Based Diagnostic Platform for Endometriosis Using Digital Droplet Enzyme-Linked Immunosorbent Assay and Single-Cell RNA Sequencing. [Abstract]2025 Apr 1:8:0652. PMID: 40171018
Purity & Documentation
References
[1]. Chambers JP, et al. Determination of serum acid phosphatase in Gaucher's disease using 4-methylumbelliferyl phosphate. Clin Chim Acta. 1977 Oct 1;80(1):67-77. [Content Brief]
[2]. Watanabe F, et al. The analysis of alkaline phosphatase isoenzyme using 4-methylumbelliferyl phosphate as substrate on a cellulose acetate membrane. Clin Chim Acta. 1979 Feb 1;91(3):273-6. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
4-Methylumbelliferyl phosphate disodium Related Classifications
HY-D0994A Related Classifications
Powered by Bioz