4-Methylumbelliferyl β-D-Glucopyranoside

Cat. No.: HY-123633 Purity: 99.83%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

4-Methylumbelliferyl β-D-Glucopyranoside, a β-D-glucoside, is a fluorogenic substrate for β-glucosidase, utilizes to assay β-glucosidase activity. 4-Methylumbelliferyl β-D-Glucopyranoside releases the highly fluorescent 4-methylumbelliferyl (4-MU), which has an emission maximum at 445-454 nm. The excitation maximum for 4-MU is pH-dependent: 330, 370, and 385 nm at pH 4.6, 7.4, and 10.4, respectively.

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4-Methylumbelliferyl β-D-Glucopyranoside Chemical Structure

CAS No. : 18997-57-4

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent
10 mM * 1 mL in DMSO ready for reconstitution	USD 55	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 55	In-stock	Estimated Time of Arrival: December 31
Solid
50 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 80	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
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Description

4-Methylumbelliferyl β-D-Glucopyranoside, a β-D-glucoside, is a fluorogenic substrate for β-glucosidase, utilizes to assay β-glucosidase activity^[1]. 4-Methylumbelliferyl β-D-Glucopyranoside releases the highly fluorescent 4-methylumbelliferyl (4-MU), which has an emission maximum at 445-454 nm. The excitation maximum for 4-MU is pH-dependent: 330, 370, and 385 nm at pH 4.6, 7.4, and 10.4, respectively^[2].

IC₅₀ & Target

4-Methylumbelliferyl β-D-Glucopyranoside is a fluorogenic substrate for β-glucosidase^[1].

Molecular Weight

338.31

Formula

C₁₆H₁₈O₈

CAS No.

18997-57-4

Appearance

Solid

Color

White to off-white

SMILES

OC[C@@H]1[C@H]([C@@H]([C@H]([C@@H](O1)OC2=CC=C3C(C)=CC(OC3=C2)=O)O)O)O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (295.59 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

DMF : 50 mg/mL (147.79 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.9559 mL	14.7793 mL	29.5587 mL
5 mM	0.5912 mL	2.9559 mL	5.9117 mL
10 mM	0.2956 mL	1.4779 mL	2.9559 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

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Dosing volume
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μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.83%

Select Batch:

Data Sheet (273 KB) SDS (393 KB)

COA (249 KB) HNMR (253 KB) LCMS (88 KB)

Handling Instructions (2659 KB)

References

[1]. Smitka CM,et al. Rapid fluorogenic assay for differentiation of the Candida parapsilosis group from other Candida spp. J Clin Microbiol. 1989;27(1):203-206. [Content Brief]

[2]. Oftedal L, et al. Validation and assessment of preanalytical factors of a fluorometric in vitro assay for glucocerebrosidase activity in human cerebrospinal fluid. Sci Rep. 2020;10(1):22098. Published 2020 Dec 16. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMF / DMSO	1 mM	2.9559 mL	14.7793 mL	29.5587 mL	73.8967 mL
	5 mM	0.5912 mL	2.9559 mL	5.9117 mL	14.7793 mL
	10 mM	0.2956 mL	1.4779 mL	2.9559 mL	7.3897 mL
	15 mM	0.1971 mL	0.9853 mL	1.9706 mL	4.9264 mL
	20 mM	0.1478 mL	0.7390 mL	1.4779 mL	3.6948 mL
	25 mM	0.1182 mL	0.5912 mL	1.1823 mL	2.9559 mL
	30 mM	0.0985 mL	0.4926 mL	0.9853 mL	2.4632 mL
	40 mM	0.0739 mL	0.3695 mL	0.7390 mL	1.8474 mL
	50 mM	0.0591 mL	0.2956 mL	0.5912 mL	1.4779 mL
	60 mM	0.0493 mL	0.2463 mL	0.4926 mL	1.2316 mL
	80 mM	0.0369 mL	0.1847 mL	0.3695 mL	0.9237 mL
	100 mM	0.0296 mL	0.1478 mL	0.2956 mL	0.7390 mL