4-Methylumbelliferyl β-D-Glucopyranoside
Based on 1 Customer Validation
4-Methylumbelliferyl β-D-Glucopyranoside, a β-D-glucoside, is a fluorogenic substrate for β-glucosidase, utilizes to assay β-glucosidase activity. 4-Methylumbelliferyl β-D-Glucopyranoside releases the highly fluorescent 4-methylumbelliferyl (4-MU), which has an emission maximum at 445-454 nm. The excitation maximum for 4-MU is pH-dependent: 330, 370, and 385 nm at pH 4.6, 7.4, and 10.4, respectively.
For research use only. We do not sell to patients.
- Purity: 99.87%
- CAS No.: 18997-57-4
- Formula: C16H18O8
- Molecular Weight:338.31
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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HY-N0187
4-Methylumbelliferone -
HY-137824
4-Methylumbelliferyl-β-D-xylopyranoside -
HY-137845
4-Methylumbelliferyl β-D-galactopyranoside -
![rel-(1R,2R,3S,4S,5R,6S)-7-Oxabicyclo[4.1.0]heptane-2,3,4,5-tetraol](https://file.medchemexpress.com/product_pic/hy-100944.gif)
HY-100944
rel-(1R,2R,3S,4S,5R,6S)-7-Oxabicyclo[4.1.0]heptane-2,3,4,5-tetraol -
HY-D0027
7-Amino-4-methylcoumarin -
HY-D0994
4-Methylumbelliferyl phosphate -
HY-N6785
Okadaic acid -
HY-137844
L-Leucine-7-amido-4-methylcoumarin hydrochloride -
HY-W008719
MPP+ iodide -
HY-16560
Camptothecin -
HY-W011411
4-Nitrophenyl a-D-glucopyranoside -
HY-137873
4-Methylumbelliferyl-α-D-Glucopyranoside
Biological Activity
Description
IC50 & Target
β-glucosidase[1]
In Vitro
The following are the experimental steps for the β-glucosidase GLB1 luciferase activity assay using 4-Methylumbelliferyl β-D-glucopyranoside as a substrate:
1. Reagent preparation
0.64 mM 4-Methylumbelliferyl β-D-glucopyranoside, 50 mM sodium citrate (pH 3.5), recombinant human GLB1 protein expressed in CHO cells.
2. Sample treatment
Incubate at 37°C for 20 minutes and terminate the reaction with alkaline stop solution.
3. Standard curve generation
Generate a standard curve using 0.01-3 nmol/tube of 4-Methylumbelliferone (HY-N0187). The results are linear over the enzyme amount range of 10-300 ng/tube.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 18997-57-4
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Appearance Solid
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Molecular Weight 338.31
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Formula C16H18O8
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Color White to off-white
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SMILES
OC[C@@H]1[C@H]([C@@H]([C@H]([C@@H](O1)OC2=CC=C3C(C)=CC(OC3=C2)=O)O)O)O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (295.59 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
DMF : 50 mg/mL (147.79 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (274 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
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Handling Instructions (2659 KB)
References
[1]. Smitka CM,et al. Rapid fluorogenic assay for differentiation of the Candida parapsilosis group from other Candida spp. J Clin Microbiol. 1989;27(1):203-206. [Content Brief]
[2]. Oftedal L, et al. Validation and assessment of preanalytical factors of a fluorometric in vitro assay for glucocerebrosidase activity in human cerebrospinal fluid. Sci Rep. 2020;10(1):22098. Published 2020 Dec 16. [Content Brief]
[3]. Boado RJ, et al. Bi-functional IgG-lysosomal enzyme fusion proteins for brain drug delivery. Sci Rep. 2019 Dec 9;9(1):18632. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMF / DMSO | 1 mM | 2.9559 mL | 14.7793 mL | 29.5587 mL | 73.8967 mL |
| 5 mM | 0.5912 mL | 2.9559 mL | 5.9117 mL | 14.7793 mL | |
| 10 mM | 0.2956 mL | 1.4779 mL | 2.9559 mL | 7.3897 mL | |
| 15 mM | 0.1971 mL | 0.9853 mL | 1.9706 mL | 4.9264 mL | |
| 20 mM | 0.1478 mL | 0.7390 mL | 1.4779 mL | 3.6948 mL | |
| 25 mM | 0.1182 mL | 0.5912 mL | 1.1823 mL | 2.9559 mL | |
| 30 mM | 0.0985 mL | 0.4926 mL | 0.9853 mL | 2.4632 mL | |
| 40 mM | 0.0739 mL | 0.3695 mL | 0.7390 mL | 1.8474 mL | |
| 50 mM | 0.0591 mL | 0.2956 mL | 0.5912 mL | 1.4779 mL | |
| 60 mM | 0.0493 mL | 0.2463 mL | 0.4926 mL | 1.2316 mL | |
| 80 mM | 0.0369 mL | 0.1847 mL | 0.3695 mL | 0.9237 mL | |
| 100 mM | 0.0296 mL | 0.1478 mL | 0.2956 mL | 0.7390 mL |
4-Methylumbelliferyl β-D-Glucopyranoside Related Classifications
HY-123633 Related Classifications
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