1. Cell Cycle/DNA Damage
    Antibody-drug Conjugate/ADC Related
  2. Topoisomerase
    ADC Cytotoxin

Campathecin (Synonyms: Camptothecin; (S)-(+)-Camptothecin; CPT)

Cat. No.: HY-16560 Purity: 98.26%
Handling Instructions

Campathecin is a potent DNA enzyme topoisomerase I inhibitor, with an IC50 of 679 nM.

For research use only. We do not sell to patients.
Campathecin Chemical Structure

Campathecin Chemical Structure

CAS No. : 7689-03-4

Size Price Stock Quantity
Free Sample (0.5-1 mg)   Apply now  
10 mM * 1 mL in DMSO USD 66 In-stock
100 mg USD 60 In-stock
500 mg USD 180 In-stock
1 g   Get quote  
5 g   Get quote  

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  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

Campathecin is a potent DNA enzyme topoisomerase I inhibitor, with an IC50 of 679 nM.

IC50 & Target

IC50: 679 nM (topoisomerase I)[3]

In Vitro

[3H]BrCPT labeling of topoisomerase I is enhanced greatly by the presence of DNA; very little labeling of isolated topoisomerase I or isolated DNA occurrs. Even in the presence of DNA, [3H]BrCPT labeling of topoisomerase I is inhibited by camptothecin, suggesting that both CPT and BrCPT bind to the same site on the DNA-topoisomerase I binary complex[1]. With increasing concentrations of camptothecin, closed circular pRR322 DNA (form I) is converted to nicked circular DNA (form 11). This apparent nicking activitv of camptothecin required DNA topoisomerase I[2].

Clinical Trial
References
Preparing Stock Solutions
Concentration Volume Mass 1 mg 5 mg 10 mg
1 mM 2.8707 mL 14.3534 mL 28.7068 mL
5 mM 0.5741 mL 2.8707 mL 5.7414 mL
10 mM 0.2871 mL 1.4353 mL 2.8707 mL
Please refer to the solubility information to select the appropriate solvent.
Kinase Assay
[1]

Each reaction mixture (200 μL, total volume) contained 2 μg of supercoiled pDPT2789 DNA (2.4 nM plasmid concentration), 50 mM Tris-HCl, pH 7.5, 120 mM KCl, 10 mM MgCl2, 0.5 mM EDTA, 0.05% dimethyl sulfoxide, 1% methanol, 100 μg/mL BSA, 4.3 ng of calf thymus topoisomerase I (0.26 nM), and a CPT derivative. The reaction mixture is incubated at 37°C, and at the indicated times 20-μL aliquots are removed and terminated by the addition of 5 μL of SDS/Ficoll stop mix (final concentrations, 0.5% SDS, 2% Ficoll, 0.025% bromphenol blue). The samples are loaded onto a 1% agarose gel and analyzed by electrophoresis. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

348.35

Formula

C₂₀H₁₆N₂O₄

CAS No.

7689-03-4

SMILES

O=C1C2=C([[email protected]@](O)(CC)C(OC2)=O)C=C(N1C3)C(C3=C4)=NC5=C4C=CC=C5

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

Solvent & Solubility

DMSO: 20.66 mg/mL (Need warming)

* "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

Purity: 98.26%

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Product Name:
Campathecin
Cat. No.:
HY-16560
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