1. Cell Cycle/DNA Damage
    Apoptosis
    Antibody-drug Conjugate/ADC Related
  2. Topoisomerase
    Apoptosis
    ADC Cytotoxin
  3. Camptothecin

Camptothecin (Synonyms: Campathecin; (S)-(+)-Camptothecin; CPT)

Cat. No.: HY-16560 Purity: 98.26%
Handling Instructions

Camptothecin (Campathecin) is a potent DNA enzyme topoisomerase I inhibitor, with an IC50 of 679 nM.

For research use only. We do not sell to patients.

Camptothecin Chemical Structure

Camptothecin Chemical Structure

CAS No. : 7689-03-4

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10 mM * 1 mL in DMSO USD 55 In-stock
Estimated Time of Arrival: December 31
100 mg USD 50 In-stock
Estimated Time of Arrival: December 31
500 mg USD 150 In-stock
Estimated Time of Arrival: December 31
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Customer Review

Based on 14 publication(s) in Google Scholar

Top Publications Citing Use of Products

    Camptothecin purchased from MCE. Usage Cited in: Nat Commun. 2018 Oct 8;9(1):4139.

    One hour after treated with UV or 2 h after treated with MMS, Camptothecin (CPT) (10 μM), or Doxorubicin (DOX; 0.5 μM), HEK293T cells expressing HA-SUMO2 and His-RhoB are subjected to sumoylation assay to detect the SUMO2 conjugation of RhoB.

    Camptothecin purchased from MCE. Usage Cited in: Nat Commun. 2019 Aug 21;10(1):3761.

    Genotoxic stress-induced β-catenin signaling is activated via a TCF- or FOXO-independent mechanism. Representative images of the subcellular localization of β-catenin in the indicated cells treated with CPT (10 μM, 1 h), IR (10 Gy), and CDDP (10 μM, 1 h), as analyzed by immunofluorescence staining.
    • Biological Activity

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    Description

    Camptothecin (Campathecin) is a potent DNA enzyme topoisomerase I inhibitor, with an IC50 of 679 nM.

    IC50 & Target[3]

    Topoisomerase I

    679 nM (IC50)

    Camptothecins

     

    In Vitro

    [3H]BrCPT labeling of topoisomerase I is enhanced greatly by the presence of DNA; very little labeling of isolated topoisomerase I or isolated DNA occurrs. Even in the presence of DNA, [3H]BrCPT labeling of topoisomerase I is inhibited by camptothecin, suggesting that both CPT and BrCPT bind to the same site on the DNA-topoisomerase I binary complex[1]. With increasing concentrations of camptothecin, closed circular pRR322 DNA (form I) is converted to nicked circular DNA (form 11). This apparent nicking activitv of camptothecin required DNA topoisomerase I[2].

    Clinical Trial
    Molecular Weight

    348.35

    Formula

    C₂₀H₁₆N₂O₄

    CAS No.

    7689-03-4

    SMILES

    O=C1C2=C([[email protected]@](O)(CC)C(OC2)=O)C=C(N1C3)C(C3=C4)=NC5=C4C=CC=C5

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    Solvent & Solubility
    In Vitro: 

    DMSO : 7.69 mg/mL (22.08 mM; Need ultrasonic)

    H2O : < 0.1 mg/mL (insoluble)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.8707 mL 14.3534 mL 28.7068 mL
    5 mM 0.5741 mL 2.8707 mL 5.7414 mL
    10 mM 0.2871 mL 1.4353 mL 2.8707 mL
    *Please refer to the solubility information to select the appropriate solvent.
    References
    Kinase Assay
    [1]

    Each reaction mixture (200 μL, total volume) contained 2 μg of supercoiled pDPT2789 DNA (2.4 nM plasmid concentration), 50 mM Tris-HCl, pH 7.5, 120 mM KCl, 10 mM MgCl2, 0.5 mM EDTA, 0.05% dimethyl sulfoxide, 1% methanol, 100 μg/mL BSA, 4.3 ng of calf thymus topoisomerase I (0.26 nM), and a CPT derivative. The reaction mixture is incubated at 37°C, and at the indicated times 20-μL aliquots are removed and terminated by the addition of 5 μL of SDS/Ficoll stop mix (final concentrations, 0.5% SDS, 2% Ficoll, 0.025% bromphenol blue). The samples are loaded onto a 1% agarose gel and analyzed by electrophoresis.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References

    Purity: 98.26%

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    Product name:
    Camptothecin
    Cat. No.:
    HY-16560
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