4-Methylumbelliferyl-α-D-Glucopyranoside
Based on 1 Customer Validation
4-Methylumbelliferyl-α-D-Glucopyranoside (4-Methylumbelliferyl-α-D-Glucose) is a fluorescent substrate for α-glucosidase, which releases the fluorescent moiety 4-methylumbelliferyl (4-MU) upon cleavage. 4-MU has pH-dependent fluorescence excitation activity, with excitation wavelengths of 320 nm at low pH (1.97-6.72) and 360 nm at high pH (7.12-10.3), respectively. The emission wavelength of 4-Methylumbelliferyl-α-D-Glucopyranoside increases with decreasing pH, ranging from 445-455 nm. 4-Methylumbelliferyl-α-D-Glucopyranoside can be used as a biomarker for Fabry and Pompe diseases to quantify α-glucosidase activity in infant blood spot samples.
For research use only. We do not sell to patients.
- Purity : 99.90%
- CAS No.: 17833-43-1
- Formula: C16H18O8
- Molecular Weight:338.31
-
Storage:
4°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
-
HY-D1632
4-MU-α-GlcNS sodium -
HY-123633
4-Methylumbelliferyl β-D-Glucopyranoside -
HY-15930
TMB -
HY-15934
X-GAL -
HY-N2526
Nervonic acid -
HY-DY2002
X-GAL (solution) -
HY-138226
BODIPY 558/568 C12 -
HY-113224
Desmosterol -
HY-15909
BCIP -
HY-DY1061
BODIPY 558/568 C12 (solution) -
HY-113224R
Desmosterol (Standard) -
HY-19332
Kifunensine
Biological Activity
Description
In Vitro
Guide (The following is our recommended protocol. This protocol is provided as a guide only and should be modified to suit your specific needs).
GAA enzyme assay[2]
1. Preparation of 4-Methylumbelliferyl-α-D-Glucopyranoside working solution
1) Prepare 2 mM 4-Methylumbelliferyl-α-D-Glucopyranoside working solution.
2. Experimental steps
1) Harvest fibroblasts and HEK293T cells by trypsinization and disrupt by freeze-thawing (3 times).
2) Mix the cell homogenate (30-40 μg protein) with 2 mM 4-Methylumbelliferyl-α-D-Glucopyranoside in 0.2 acetate buffer (pH 4.0) and incubate at 37° for 60 min.
3) The reaction is stopped with 1.9 mL of glycine carbonate buffer (pH 10.7) and fluorescence is read on a Turner Biosystems Modulus 9200 fluorometer (360 nm excitation, 450 nm emission).
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
CAS No. 17833-43-1
-
Appearance Solid
-
Molecular Weight 338.31
-
Formula C16H18O8
-
Color White to off-white
-
SMILES
O=C1C=C(C)C2=CC=C(O[C@@H]3[C@@H]([C@H]([C@@H]([C@@H](CO)O3)O)O)O)C=C2O1
-
Synonyms
4-Methylumbelliferyl-α-D-Glucose; 4-Methylumbelliferyl-α-D-Glucoside; 4-MU-α-D-Glucopyranoside
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
4°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
Solvent & Solubility
In Vitro:
DMSO : 25 mg/mL (73.90 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (7.39 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (7.39 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
-
Data Sheet (275 KB)
-
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
-
Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.9559 mL | 14.7793 mL | 29.5587 mL | 73.8967 mL |
| 5 mM | 0.5912 mL | 2.9559 mL | 5.9117 mL | 14.7793 mL | |
| 10 mM | 0.2956 mL | 1.4779 mL | 2.9559 mL | 7.3897 mL | |
| 15 mM | 0.1971 mL | 0.9853 mL | 1.9706 mL | 4.9264 mL | |
| 20 mM | 0.1478 mL | 0.7390 mL | 1.4779 mL | 3.6948 mL | |
| 25 mM | 0.1182 mL | 0.5912 mL | 1.1823 mL | 2.9559 mL | |
| 30 mM | 0.0985 mL | 0.4926 mL | 0.9853 mL | 2.4632 mL | |
| 40 mM | 0.0739 mL | 0.3695 mL | 0.7390 mL | 1.8474 mL | |
| 50 mM | 0.0591 mL | 0.2956 mL | 0.5912 mL | 1.4779 mL | |
| 60 mM | 0.0493 mL | 0.2463 mL | 0.4926 mL | 1.2316 mL |
4-Methylumbelliferyl-α-D-Glucopyranoside Related Classifications
HY-137873 Related Classifications
Powered by Bioz