4-Methylumbelliferyl-α-D-Glucopyranoside  (Synonyms: 4-Methylumbelliferyl-α-D-Glucose; 4-Methylumbelliferyl-α-D-Glucoside; 4-MU-α-D-Glucopyranoside)

4-Methylumbelliferyl-α-D-Glucopyranoside (4-Methylumbelliferyl-α-D-Glucose) is a fluorescent substrate for α-glucosidase, which releases the fluorescent moiety 4-methylumbelliferyl (4-MU) upon cleavage. 4-MU has pH-dependent fluorescence excitation activity, with excitation wavelengths of 320 nm at low pH (1.97-6.72) and 360 nm at high pH (7.12-10.3), respectively. The emission wavelength of 4-Methylumbelliferyl-α-D-Glucopyranoside increases with decreasing pH, ranging from 445-455 nm. 4-Methylumbelliferyl-α-D-Glucopyranoside can be used as a biomarker for Fabry and Pompe diseases to quantify α-glucosidase activity in infant blood spot samples.

Guide (The following is our recommended protocol. This protocol is provided as a guide only and should be modified to suit your specific needs).
GAA enzyme assay^[2]
1. Preparation of 4-Methylumbelliferyl-α-D-Glucopyranoside working solution
1) Prepare 2 mM 4-Methylumbelliferyl-α-D-Glucopyranoside working solution.
2. Experimental steps
1) Harvest fibroblasts and HEK293T cells by trypsinization and disrupt by freeze-thawing (3 times).
2) Mix the cell homogenate (30-40 μg protein) with 2 mM 4-Methylumbelliferyl-α-D-Glucopyranoside in 0.2 acetate buffer (pH 4.0) and incubate at 37° for 60 min.
3) The reaction is stopped with 1.9 mL of glycine carbonate buffer (pH 10.7) and fluorescence is read on a Turner Biosystems Modulus 9200 fluorometer (360 nm excitation, 450 nm emission).

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

338.31

C₁₆H₁₈O₈

17833-43-1

Solid

White to off-white

O=C1C=C(C)C2=CC=C(O[C@@H]3[C@@H]([C@H]([C@@H]([C@@H](CO)O3)O)O)O)C=C2O1

Room temperature in continental US; may vary elsewhere.

4°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

• [1]. Zhi, et al. Fluorescent properties of hymecromone and fluorimetric analysis of hymecromone in compound dantong capsule. J. Spectrosc. 147128 (2013).

  [2]. Parenti G, et al. Pharmacological Enhancement of Mutated α-Glucosidase Activity in Fibroblasts from Patients with Pompe Disease. Mol Ther. 2007 Mar;15(3):508-514.  [Content Brief]