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  4. 4-Methylumbelliferyl-β-D-xylopyranoside

4-Methylumbelliferyl-β-D-xylopyranoside  (Synonyms: 4-Methylumbelliferyl-β-D-xyloside)

Cat. No.: HY-137824 Purity: 99.70%
COA
SDS
Handling Instructions Technical Support

4-Methylumbelliferyl-β-D-xylopyranoside (4-Methylumbelliferyl-β-D-xyloside) is an enzymatically hydrolyzable β-D-xylopyranoside substrate. The enzymatic hydrolysis of 4-Methylumbelliferyl-β-D-xylopyranoside is mediated by the stereochemistry-retaining β-D-xylosidase GSXynB2 via a two-step catalytic process (xylosylation followed by dexylosidation). 4-Methylumbelliferyl-β-D-xylopyranoside serves as a substrate for the β-xylosidase of Trichoderma reesei QM 9414, and its enzymatic hydrolysis process is limited by the dexylosidation step.

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4-Methylumbelliferyl-β-D-xylopyranoside

4-Methylumbelliferyl-β-D-xylopyranoside 화학구조

CAS No. : 6734-33-4

사이즈 가격 재고 수량
무료 샘플 (0.1 - 0.2 mg)   지금 신청하기  
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 해외재고보유
Solution
10 mM * 1 mL in DMSO 해외재고보유
Solid
50 mg 해외재고보유
100 mg 해외재고보유
250 mg 해외재고보유
500 mg   견적 받기  
1 g   견적 받기  

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4-Methylumbelliferyl-β-D-xylopyranoside Related Antibodies

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제품 설명

4-Methylumbelliferyl-β-D-xylopyranoside (4-Methylumbelliferyl-β-D-xyloside) is an enzymatically hydrolyzable β-D-xylopyranoside substrate. The enzymatic hydrolysis of 4-Methylumbelliferyl-β-D-xylopyranoside is mediated by the stereochemistry-retaining β-D-xylosidase GSXynB2 via a two-step catalytic process (xylosylation followed by dexylosidation). 4-Methylumbelliferyl-β-D-xylopyranoside serves as a substrate for the β-xylosidase of Trichoderma reesei QM 9414, and its enzymatic hydrolysis process is limited by the dexylosidation step.

In Vitro

4-Methylumbelliferyl-β-D-xylopyranoside (pH 7.0; 25 °C) is hydrolyzed by purified GSXynB2 from Geobacillus stearothermophilus T-6 with a kcat of 12.6 s−1, with the dexylosylation step of the catalytic cycle acting as the primary rate-limiting step[1].
4-Methylumbelliferyl-β-D-xylopyranoside (1.31 mM; pH 7.0; 25 °C) rapidly forms a covalent enzyme-xylose intermediate with purified GSXynB2 from Geobacillus stearothermophilus T-6, as evidenced by a presteady-state burst phase with a rate constant of 579 s−1[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

4-Methylumbelliferyl-β-D-xylopyranoside Related Antibodies
분자량

308.29

화학식

C15H16O7
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

CC(C(C(O1)=C2)=CC=C2O[C@H](OC[C@H]3O)[C@@H]([C@H]3O)O)=CC1=O
Structure Classification
Initial Source

human
선적

Room temperature in continental US; may vary elsewhere.
보관
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
용액&용해도
In Vitro: 

DMSO : 125 mg/mL (405.46 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.2437 mL 16.2185 mL 32.4370 mL
5 mM 0.6487 mL 3.2437 mL 6.4874 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

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(per animal)

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Dosing volume
(per animal)

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Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
순도&문서

Purity: 99.70%

SDS (394 KB)

COA (249 KB) HNMR (254 KB) LCMS (88 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.2437 mL 16.2185 mL 32.4370 mL 81.0925 mL
5 mM 0.6487 mL 3.2437 mL 6.4874 mL 16.2185 mL
10 mM 0.3244 mL 1.6218 mL 3.2437 mL 8.1092 mL
15 mM 0.2162 mL 1.0812 mL 2.1625 mL 5.4062 mL
20 mM 0.1622 mL 0.8109 mL 1.6218 mL 4.0546 mL
25 mM 0.1297 mL 0.6487 mL 1.2975 mL 3.2437 mL
30 mM 0.1081 mL 0.5406 mL 1.0812 mL 2.7031 mL
40 mM 0.0811 mL 0.4055 mL 0.8109 mL 2.0273 mL
50 mM 0.0649 mL 0.3244 mL 0.6487 mL 1.6218 mL
60 mM 0.0541 mL 0.2703 mL 0.5406 mL 1.3515 mL
80 mM 0.0405 mL 0.2027 mL 0.4055 mL 1.0137 mL
100 mM 0.0324 mL 0.1622 mL 0.3244 mL 0.8109 mL
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4-Methylumbelliferyl-β-D-xylopyranoside Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

4-Methylumbelliferyl-β-D-xylopyranoside6734-33-44-Methylumbelliferyl-β-D-xylosideBiochemical Assay Reagentsxylosylationenzymatic hydrolysisTrichoderma reesei QM 9414Geobacillus stearothermophilus T-6covalent enzyme-xylose intermediateβ-D-xylopyranoside substrateβ-D-xylosidasepresteady-state kinetic experimentsGSXynB2dexylosidationInhibitorinhibitorinhibit

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상품명:
4-Methylumbelliferyl-β-D-xylopyranoside
Cat. No.:
HY-137824
수량:
MCE Japan Authorized Agent:

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