4-Methylumbelliferyl-β-D-xylopyranoside
Based on 1 Customer Validation
4-Methylumbelliferyl-β-D-xylopyranoside (4-Methylumbelliferyl-β-D-xyloside) is an enzymatically hydrolyzable β-D-xylopyranoside substrate. The enzymatic hydrolysis of 4-Methylumbelliferyl-β-D-xylopyranoside is mediated by the stereochemistry-retaining β-D-xylosidase GSXynB2 via a two-step catalytic process (xylosylation followed by dexylosidation). 4-Methylumbelliferyl-β-D-xylopyranoside serves as a substrate for the β-xylosidase of Trichoderma reesei QM 9414, and its enzymatic hydrolysis process is limited by the dexylosidation step.
For research use only. We do not sell to patients.
- Purity : 99.70%
- CAS No.: 6734-33-4
- Formula: C15H16O7
- Molecular Weight:308.29
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Description
In Vitro
4-Methylumbelliferyl-β-D-xylopyranoside (pH 7.0; 25 °C) is hydrolyzed by purified GSXynB2 from Geobacillus stearothermophilus T-6 with a kcat of 12.6 s−1, with the dexylosylation step of the catalytic cycle acting as the primary rate-limiting step[1].
4-Methylumbelliferyl-β-D-xylopyranoside (1.31 mM; pH 7.0; 25 °C) rapidly forms a covalent enzyme-xylose intermediate with purified GSXynB2 from Geobacillus stearothermophilus T-6, as evidenced by a presteady-state burst phase with a rate constant of 579 s−1[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. .
Chemical Information
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CAS No. 6734-33-4
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Appearance Solid
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Molecular Weight 308.29
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Formula C15H16O7
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Color White to off-white
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SMILES
CC(C(C(O1)=C2)=CC=C2O[C@H](OC[C@H]3O)[C@@H]([C@H]3O)O)=CC1=O
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Synonyms
4-Methylumbelliferyl-β-D-xyloside
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Structure Classification
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Initial Source
human
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
In Vitro:
DMSO : 125 mg/mL (405.46 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (274 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
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Handling Instructions (2659 KB)
References
[1]. Jordan DB, et al. Rate-limiting steps of a stereochemistry retaining β-d-xylosidase from Geobacillus stearothermophilus acting on four substrates. Arch Biochem Biophys. 2015 Oct 1;583:73-8. [Content Brief]
[2]. Gómez M, et al. Chemical mechanism of beta-xylosidase from Trichoderma reesei QM 9414: pH-dependence of kinetic parameters. Biochimie. 2001;83(10):961-967. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.2437 mL | 16.2185 mL | 32.4370 mL | 81.0925 mL |
| 5 mM | 0.6487 mL | 3.2437 mL | 6.4874 mL | 16.2185 mL | |
| 10 mM | 0.3244 mL | 1.6218 mL | 3.2437 mL | 8.1092 mL | |
| 15 mM | 0.2162 mL | 1.0812 mL | 2.1625 mL | 5.4062 mL | |
| 20 mM | 0.1622 mL | 0.8109 mL | 1.6218 mL | 4.0546 mL | |
| 25 mM | 0.1297 mL | 0.6487 mL | 1.2975 mL | 3.2437 mL | |
| 30 mM | 0.1081 mL | 0.5406 mL | 1.0812 mL | 2.7031 mL | |
| 40 mM | 0.0811 mL | 0.4055 mL | 0.8109 mL | 2.0273 mL | |
| 50 mM | 0.0649 mL | 0.3244 mL | 0.6487 mL | 1.6218 mL | |
| 60 mM | 0.0541 mL | 0.2703 mL | 0.5406 mL | 1.3515 mL | |
| 80 mM | 0.0405 mL | 0.2027 mL | 0.4055 mL | 1.0137 mL | |
| 100 mM | 0.0324 mL | 0.1622 mL | 0.3244 mL | 0.8109 mL |
4-Methylumbelliferyl-β-D-xylopyranoside Related Classifications
HY-137824 Related Classifications
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Keywords
- 4-Methylumbelliferyl-β-D-xylopyranoside
- 6734-33-4
- 4-Methylumbelliferyl-β-D-xyloside
- Biochemical Assay Reagents
- xylosylation
- enzymatic hydrolysis
- Trichoderma reesei QM 9414
- Geobacillus stearothermophilus T-6
- covalent enzyme-xylose intermediate
- β-D-xylopyranoside substrate
- β-D-xylosidase
- presteady-state kinetic experiments
- GSXynB2
- dexylosidation
- Inhibitor
- inhibitor
- inhibit