L-Leucine-7-amido-4-methylcoumarin hydrochloride
Based on 1 Customer Validation
L-Leucine-7-amido-4-methylcoumarin (Leu-AMC) hydrochloride is a bright blue fluorogenic peptidyl substrate for LAP3 (leucine aminopeptidase). L-Leucine-7-amido-4-methylcoumarin hydrochloride can be used for leucine aminopeptidase inhibition assays in vitro.
For research use only. We do not sell to patients.
- Purity : 99.98%
- CAS No.: 62480-44-8
- Formula: C16H21ClN2O3
- Molecular Weight:324.80
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Storage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Description
In Vitro
In leucine aminopeptidases inhibition assays in KB 3.1 cells. Compounds3and5are observed to weakly inhibit the biofilm formation byS. aureusand constrain inhibitory activity of -Leu-AMC hydrolysis in KB 3.1 cells[1].The amount of hydrolyzed 7-amino-4-methylcoumarin (AMC) is determined in a fluorescence spectrophotometer (Ex/Em=365/440 nm)[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 62480-44-8
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Appearance Solid
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Molecular Weight 324.80
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Formula C16H21ClN2O3
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Color White to off-white
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SMILES
CC(C(C(O1)=C2)=CC=C2NC([C@@H](N)CC(C)C)=O)=CC1=O.Cl
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Synonyms
Leu-AMC hydrochloride
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (307.88 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (270 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.0788 mL | 15.3941 mL | 30.7882 mL | 76.9704 mL |
| 5 mM | 0.6158 mL | 3.0788 mL | 6.1576 mL | 15.3941 mL | |
| 10 mM | 0.3079 mL | 1.5394 mL | 3.0788 mL | 7.6970 mL | |
| 15 mM | 0.2053 mL | 1.0263 mL | 2.0525 mL | 5.1314 mL | |
| 20 mM | 0.1539 mL | 0.7697 mL | 1.5394 mL | 3.8485 mL | |
| 25 mM | 0.1232 mL | 0.6158 mL | 1.2315 mL | 3.0788 mL | |
| 30 mM | 0.1026 mL | 0.5131 mL | 1.0263 mL | 2.5657 mL | |
| 40 mM | 0.0770 mL | 0.3849 mL | 0.7697 mL | 1.9243 mL | |
| 50 mM | 0.0616 mL | 0.3079 mL | 0.6158 mL | 1.5394 mL | |
| 60 mM | 0.0513 mL | 0.2566 mL | 0.5131 mL | 1.2828 mL | |
| 80 mM | 0.0385 mL | 0.1924 mL | 0.3849 mL | 0.9621 mL | |
| 100 mM | 0.0308 mL | 0.1539 mL | 0.3079 mL | 0.7697 mL |
L-Leucine-7-amido-4-methylcoumarin hydrochloride Related Classifications
HY-137844 Related Classifications
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