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4-Methylumbelliferyl oleate 

Cat. No.: HY-117095
Handling Instructions

4-Methylumbelliferyl oleate is a fluorogenic substrate for acid and alkaline lipases. 4-Methylumbelliferyl oleate is cleaved by lipases, liberating 4-Methylumbelliferyl (Ex/Em=320/450 nm).

For research use only. We do not sell to patients.

4-Methylumbelliferyl oleate Chemical Structure

4-Methylumbelliferyl oleate Chemical Structure

CAS No. : 18323-58-5

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Description

4-Methylumbelliferyl oleate is a fluorogenic substrate for acid and alkaline lipases. 4-Methylumbelliferyl oleate is cleaved by lipases, liberating 4-Methylumbelliferyl (Ex/Em=320/450 nm)[1][2].

In Vitro

The inhibitory activity against pancreatic lipase was measured using 4-Methylumbelliferyl (4-MU) oleate as a substrate. The reaction mixture consisted of 50 µL 0.1 mM 4-MU oleate, 20 µL McIlvane buffer (0.1 M citrate-Na2 HPO4, pH 7.4), and 5 µL of sample solution. Porcine pancreatic lipase (25 µL) was added to the reaction mixture and the final volume was adjusted to 0.1 ml. After the mixture was incubated at 37℃ for 10 min, the amount of 4-MU released by the lipase was measured using a fluorescence multi-detection reader at an excitation wavelength of 320 nm and an emission wavelength of 450 nm[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

440.61

Formula

C28H40O4

CAS No.
SMILES

O=C1C=C(C2=C(O1)C=C(C=C2)OC(CCCCCCC/C=C\CCCCCCCC)=O)C

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

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Product Name:
4-Methylumbelliferyl oleate
Cat. No.:
HY-117095
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