4-Methylumbelliferyl oleate
Based on 1 Customer Validation
4-Methylumbelliferyl oleate is a fluorogenic substrate for acid and alkaline lipases. 4-Methylumbelliferyl oleate is cleaved by lipases, liberating 4-Methylumbelliferyl (Ex/Em=320/450 nm).
For research use only. We do not sell to patients.
- Purity: 99.58%
- CAS No.: 18323-58-5
- Formula: C28H40O4
- Molecular Weight:440.61
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Storage:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Guide (The following is our recommended solution. This solution is merely a guideline and should be modified according to your specific needs.)
1. Prepare the reaction system: Add the following components in sequence to the microplate or reaction tube:
50 μL 0.1 mM 4-MU oleate (substrate)
20 μL McIlvane buffer (pH 7.4)
5 μL sample solution to be tested (dissolved in an appropriate solvent)
2. Add the enzyme solution: Add 25 μL porcine pancreatic lipase to initiate the reaction.
3. Volume Adjustment: If necessary, add buffer solution or deionized water to replenish the total volume of the reaction to 100 μL.
4. Incubation: Place the reaction system at 37°C for 10 minutes.
5. Detection: Measure the fluorescence intensity of the released 4-methylumbelliferone (4-MU).
Excitation wavelength: 320 nm
Emission wavelength: 450 nm
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 18323-58-5
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Appearance Solid-Liquid Mixture
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Molecular Weight 440.61
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Formula C28H40O4
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Color White to off-white
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SMILES
O=C1C=C(C2=C(O1)C=C(C=C2)OC(CCCCCCC/C=C\CCCCCCCC)=O)C
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 100 mg/mL (226.96 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (267 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Zhang J, et al. Pancreatic lipase inhibitory activity of taraxacum officinale in vitro and in vivo. Nutr Res Pract. 2008 Winter;2(4):200-3. [Content Brief]
[2]. Koster JF, et al. Study of the hydrolysis of 4-methylumbelliferyl oleate by acid lipase and cholesteryl oleate by acid cholesteryl esterase in human leucocytes, fibroblasts and liver. Biochim Biophys Acta. 1980 Apr 18;618(1):98-105. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.2696 mL | 11.3479 mL | 22.6958 mL | 56.7395 mL |
| 5 mM | 0.4539 mL | 2.2696 mL | 4.5392 mL | 11.3479 mL | |
| 10 mM | 0.2270 mL | 1.1348 mL | 2.2696 mL | 5.6740 mL | |
| 15 mM | 0.1513 mL | 0.7565 mL | 1.5131 mL | 3.7826 mL | |
| 20 mM | 0.1135 mL | 0.5674 mL | 1.1348 mL | 2.8370 mL | |
| 25 mM | 0.0908 mL | 0.4539 mL | 0.9078 mL | 2.2696 mL | |
| 30 mM | 0.0757 mL | 0.3783 mL | 0.7565 mL | 1.8913 mL | |
| 40 mM | 0.0567 mL | 0.2837 mL | 0.5674 mL | 1.4185 mL | |
| 50 mM | 0.0454 mL | 0.2270 mL | 0.4539 mL | 1.1348 mL | |
| 60 mM | 0.0378 mL | 0.1891 mL | 0.3783 mL | 0.9457 mL | |
| 80 mM | 0.0284 mL | 0.1418 mL | 0.2837 mL | 0.7092 mL | |
| 100 mM | 0.0227 mL | 0.1135 mL | 0.2270 mL | 0.5674 mL |
4-Methylumbelliferyl oleate Related Classifications
HY-117095 Related Classifications
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