C12FDG
Based on 9 publication(s) in Google Scholar
C12FDG (5-Dodecanoylaminofluorescein di-β-D-Galactopyranoside) is a lipophilic green fluorescent substrate for β-galactosidase detection. C12-FDG is more sensitive than Fluorescein di(β-D-galactopyranoside) (HY-101895) for beta-galactosidase activity determinations in animal cells (Ex/Em = 488/523 nm).
For research use only. We do not sell to patients.
- Purity: 99.82%
- CAS No.: 138777-25-0
- Formula: C44H55NO16
- Molecular Weight:853.90
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) C12FDG
More- Sci Adv. 2025 Jul 11;11(28):eadw1553. [Abstract]
- Cell Rep Med. 2025 Sep 19:102365. [Abstract]
- Cell Rep Med. 2025 Aug 20:102310. [Abstract]
- Phytomedicine. 2026 Jan 22:153:157782. [Abstract]
- Free Radic Biol Med. 2025 Dec 23:244:505-515. [Abstract]
- Mol Oncol. 2025 Mar 14. [Abstract]
- bioRxiv. 2026 Feb 12.
- University of Padua. 2025.
- bioRxiv. 2025 April 26.
Customer Validation & Images
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IF
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Flow Cytometry
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IF
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Bio/Physico-chemical Assay
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Cell Imaging/Staining
Biological Activity
Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Solution preparation[2]
1.1 Prepare storage solution
Solvent: DMSO
Concentration: 33 mM is recommended (e.g., weigh 1 mg C12FDG and dissolve it in 35.5 μL solvent).
Storage: After aliquoting, store at -20°C or -80°C in the dark and avoid repeated freezing and thawing.
1.2 Preparation of working solution
Dilute to 33 μM (optimized according to the experiment) with PBS (or other buffer).
Note: The working solution should be prepared and used immediately, and protected from light.
2. Fluorescent senescence-associated β-galactosidase (SA-β-Gal) assay:
2.1 Culture cells in 6-, 12- , 24-, or 96-well plates at a density of 5× 105 cells/mL overnight. Incubate the cells according to your normal protocol.
2.2 Wash cells by 200 μL of PBS once and fix with 100 μL of fixation solution (2% formaldehyde/0.2% glutaraldehyde in distilled water) at room temperature for 5 min.
2.3 Wash cells by 200 μL of PBS two times, and stain with 100 μL of 33 μM C12FDG (in PBS, pH=6.0) for 10 min, and with 200 μL of Hoechst solution (1 μg/mL Hoechst 33342 (HY-15559) in PBS, pH 6.0) for 10 min.
2.
4 Image these cells by a 360-nm (Hoechst 33342) and 480-nm (C12FDG) excitation filters, and monitor through 460-nm and 535-nm emission filters, respectively.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
523
488
Chemical Information
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CAS No. 138777-25-0
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Appearance Solid
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Molecular Weight 853.90
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Formula C44H55NO16
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Color White to yellow
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SMILES
CCCCCCCCCCCC(NC1=CC2=C(C3(C4=C(OC5=C3C=CC(O[C@H]6[C@@H]([C@H]([C@H]([C@@H](CO)O6)O)O)O)=C5)C=C(O[C@H]7[C@@H]([C@H]([C@H]([C@@H](CO)O7)O)O)O)C=C4)OC2=O)C=C1)=O
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Synonyms
5-Dodecanoylaminofluorescein di-β-D-Galactopyranoside
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (9)
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Journal Impact Factor
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Most Recent
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Sci Adv
Transition of cellular senescence to pyroptosis mediates recurrence of small cell lung cancer after chemotherapy. [Abstract]2025 Jul 11;11(28):eadw1553. PMID: 40644541
C12FDG purchased from MedChemExpress. Usage Cited in: Sci Adv. 2025 Jul 11;11(28):eadw1553. [Abstract]
Immunofluorescence (IF) images of C12FDG (33 μM; 30 min)-positive and N-GSDMD–positive cells in NCI-H446 on day W8, the distribution was calculated in 174 senescent cells (scale bar, 20 μm).
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Cell Rep Med
FLT3L-based drug conjugate effectively targets chemoresistant leukemia stem cells in acute myeloid leukemia. [Abstract]2025 Sep 19:102365. PMID: 40975063
C12FDG purchased from MedChemExpress. Usage Cited in: Cell Rep Med. 2025 Sep 19:102365. [Abstract]
β-galactosidase activity (C12-FDG (33 μM; 2 h)) in AML cells treated by cytarabine at indicated concentrations for 6 days.
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Cell Rep Med
An age-related decrease in leptin contributes to CD8+ T cell aging in the tumor microenvironment. [Abstract]2025 Aug 20:102310. PMID: 40858107
C12FDG purchased from MedChemExpress. Usage Cited in: Cell Rep Med. 2025 Aug 20:102310. [Abstract]
Flow cytometric analysis of the expression of p16, the level of C12FDG (33 μM; 2 h, a lipophilic green fluorescent substrate used to detect the activity of β-galactosidase), and the percentage of IFN-γ in tumor-infiltrating CD8+ T cells from Lepr+/+Cd4-Cre and Leprfl/flCd4-Cre mice aged 3–4 months injected s.c. with MC38 tumor cells (n = 5).
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Phytomedicine
Afzelin resists UVA damage through autophagy and synergizes with ganoderic acid A to skin photoaging. [Abstract]2026 Jan 22:153:157782. PMID: 41666510 -
Free Radic Biol Med
2025 Dec 23:244:505-515. PMID: 41448257
C12FDG purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2025 Dec 23:244:505-515. [Abstract]
Acceleration of cellular senescence in CMIT/MIT-exposed H441 cells. To evaluate the effect of CMIT/MIT on senescence, representative senescence markers were measured in H441 cells after 24 h of CMIT/MIT exposure. SA-β-gal activity was measured by measuring the fluorescence intensity of C12FDG (10 μM; 15 min) in CMIT/MIT-exposed H441 cells by spectrophotomete.
C12FDG purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2025 Dec 23:244:505-515. [Abstract]
Acceleration of cellular senescence in CMIT/MIT-exposed H441 cells. To evaluate the effect of CMIT/MIT on senescence, representative senescence markers were measured in H441 cells after 24 h of CMIT/MIT exposure. SA-β-gal activity was measured by measuring the fluorescence intensity of C12FDG (10 μM; 15 min) in CMIT/MIT-exposed H441 cells by confocal microscopy.
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Mol Oncol
Escape from TGF-β-induced senescence promotes aggressive hallmarks in epithelial hepatocellular carcinoma cells. [Abstract]2025 Mar 14. PMID: 40083231 -
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Solvent & Solubility
DMSO : 50 mg/mL (58.55 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (276 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
[1]. Plovins A, et al. Use of fluorescein-di-beta-D-galactopyranoside (FDG) and C12-FDG as substrates for beta-galactosidase detection by flow cytometry in animal, bacterial, and yeast cells. Appl Environ Microbiol. 1994 Dec;60(12):4638-41. [Content Brief]
[2]. Udono M, et al. Quantitative analysis of cellular senescence phenotypes using an imaging cytometer. Methods. 2012 Mar;56(3):383-8. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.1711 mL | 5.8555 mL | 11.7110 mL | 29.2774 mL |
| 5 mM | 0.2342 mL | 1.1711 mL | 2.3422 mL | 5.8555 mL | |
| 10 mM | 0.1171 mL | 0.5855 mL | 1.1711 mL | 2.9277 mL | |
| 15 mM | 0.0781 mL | 0.3904 mL | 0.7807 mL | 1.9518 mL | |
| 20 mM | 0.0586 mL | 0.2928 mL | 0.5855 mL | 1.4639 mL | |
| 25 mM | 0.0468 mL | 0.2342 mL | 0.4684 mL | 1.1711 mL | |
| 30 mM | 0.0390 mL | 0.1952 mL | 0.3904 mL | 0.9759 mL | |
| 40 mM | 0.0293 mL | 0.1464 mL | 0.2928 mL | 0.7319 mL | |
| 50 mM | 0.0234 mL | 0.1171 mL | 0.2342 mL | 0.5855 mL |
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