Hoechst 33342

Hoechst 33342 purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2024 Mar 8;15(3):200. [Abstract]

Customer Validation
C2C12 Cells were incubated with 1.25 μM MitoSOX in HBSS Ca/Mg for 10 min in dark at 37 °C with a 5% CO₂ atmosphere. Then, cell nuclei were stained by incubating with 1 µg/mL Hoechst in HBSS, for 10 min at 37 °C in the dark, with a 5% CO2 atmosphere.

Hoechst 33342 purchased from MedChemExpress. Usage Cited in: Bioact Mater. 2022 Mar 17;18:91-103. [Abstract]

Customer Validation
The fluorescent images of HUVECs stained with NO fluorescent probe (DAF-FM DA) and Hoechst 33342 after being treated with UCATS under dark conditions or 808 nm NIR irradiation.

Hoechst 33342 purchased from MedChemExpress. Usage Cited in: Chem Eng J. 15 December 2022, 138004.

Customer Validation
Apoptotic cells in four groups were stained with Hoechst 33342 (for 10 min) reflected by bright blue nuclei, including hP-MSCs cultured on noncoated, L-Gel-coated (10 nM), and D-Gel coated (10 nM) plates under exposure of 500 μM H2O2 for 5 h except for the normal group, and PBS as control.

Hoechst 33342 purchased from MedChemExpress. Usage Cited in: J Orthop Translat. 2022 Oct 17:38:23-31. [Abstract]

Customer Validation
OBs (red) and OCs were co-cultured, FAM-SGPA (green) were added to culture dish, incubated for 24 h, labelled the nucleus with Hoechst 33342 (blue, 15 min), and observed under a confocal laser scanning microscope (magnification: 630 × ).

Hoechst 33342 purchased from MedChemExpress. Usage Cited in: J Orthop Translat. 2022 Oct 17:38:23-31. [Abstract]

Customer Validation
FAM-SGPA (green) interfered with OBs for 0.5 h, 1 h, 2 h and 4 h, labelled the nucleus with Hoechst 33342 (blue, 15 min), and observed under a confocal laser scanning microscope (magnification: 400 × ).

Hoechst 33342 purchased from MedChemExpress. Usage Cited in: J Ethnopharmacol. 2022 May 10:289:115039. [Abstract]

Customer Validation
MCF-7 cells were treated with QA root extract for 48 h and observed by fluorescence microscopy after Hoechst 33342 trihydrochloride (Hoechst, 1 μg/mL; 10 min) staining. Scale bar 100 μm.

Hoechst 33342 purchased from MedChemExpress. Usage Cited in: J Lipid Res. 2022 May;63(5):100207. [Abstract]

Customer Validation
The labeling efficiency of BODIPY-loaded PLGA NPs in vitro. Fluorescence confocal images of labeled LDs in macrophages; LDs (green color, BODIPY) and nuclei (blue color, Hoechst 33342 (2 μg/mL; 2 min)); the scale bar represents 25 μm.

Hoechst 33342 purchased from MedChemExpress. Usage Cited in: Theranostics. 2021 Mar 4;11(10):4655-4671. [Abstract]

Customer Validation
Hoechst 33342 (10 µg/mL) is added to the medium of cultured megakaryocytes for 2 h at 37°C. Cells are stained with the directly coupled monoclonal antibodies PE-anti-CD41 and APC-anti-Mpl for 30 min at 4°C. Ploidy is measured in the CD41+Mpl+ cell population.

Hoechst 33342 purchased from MedChemExpress. Usage Cited in: Stem Cell Res Ther. 2021 Jan 22;12(1):77. [Abstract]

Customer Validation
To evaluate the anti-apoptosis property of extracellular vesicles from hP-MSCs, the Hoechst 33342 (for 10 min) is used to distinguish the apoptotic cells in the three different groups.

Hoechst 33342 purchased from MedChemExpress. Usage Cited in: J Cell Biol. 2021 Oct 4;220(10):e202011153. [Abstract]

Customer Validation
The released centromere parts are marked by arrows (blue: Hoechst; green: MajSAT-mClover; red: H2B-mCherry). Chromatin was briefly counterstained with Hoechst 33342 (5 µg/mL) before imaging. Scale bar: 5 µm (yellow) and 2 µm (white).

Hoechst 33342 purchased from MedChemExpress. Usage Cited in: Acta Biomater. 2020 Sep 1;113:289-304. [Abstract]

Customer Validation
Hoechst 33342 staining (for 6 h) is used to distinguish apoptotic changes in H2O2-treated hP-MSCs.

Hoechst 33342 purchased from MedChemExpress. Usage Cited in: ACS Appl Mater Interfaces. 2018 Sep 12;10(36):30081-30091. [Abstract]

Customer Validation
Hoechst 33342 (10 min) staining shows the survival of HUVECs administrated 100 μg/mL exosomes or CS-Exo upon exposure to 400 μM H₂O₂.

Hoechst 33342 purchased from MedChemExpress. Usage Cited in: Cancer Biol Ther. 2018;19(12):1193-1203. [Abstract]

Customer Validation
PC-3 stable subline cells as indicated (empty vector or CRMP4a) were seeded on cover glass in full culture media for 24 h and then stained with iFluor555-conjugated phalloidin (red) and Hoechst33342 (blue). Cells were also subjected to immunocytofluorescent staining with anti-vinculin antibodies (green) followed by visualization with AlexaFluor®488-labeld secondary antibodies. The representative microscopic images were shown from four independent experiments.