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  3. Hoechst 33342

Hoechst 33342 (Synonyms: bisBenzimide H 33342; HOE 33342)

Cat. No.: HY-15559 Purity: 99.24%
Handling Instructions

Hoechst 33342 is a DNA minor groove binder used fluorochrome for visualizing cellular DNA.

For research use only. We do not sell to patients.

Hoechst 33342 Chemical Structure

Hoechst 33342 Chemical Structure

CAS No. : 23491-52-3

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Top Publications Citing Use of Products

    Hoechst 33342 purchased from MCE. Usage Cited in: Chinese Pharmacological Bulletin. 2018 May; 34(5): 620-626.

    PC12 cells are treated with 1.5 mM Methylglyoxal (MG) for 24 h before pretreatment with Butein (2.5, 5 μM) for 1 h. Propidium iodide (PI) and Hoechst 33342 are used to determine cell apoptosis.

    Hoechst 33342 purchased from MCE. Usage Cited in: ACS Appl Mater Interfaces. 2018 Sep 12;10(36):30081-30091.

    Hoechst 33342 staining shows the survival of HUVECs administrated 100 μg/mL exosomes or CS-Exo upon exposure to 400 μM H2O2.
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    Hoechst 33342 is a DNA minor groove binder used fluorochrome for visualizing cellular DNA.

    IC50 & Target

    Dye reagent[1]
    DNA Stain[1]

    In Vitro

    Hoechst 33342 binds to adenine-thymine-rich regions of DNA in the minor groove. On binding to DNA, the fluorescence greatly increases. This protocol describes the use of Hoechst 33342 to label nuclear DNA of cells grown in culture. Hoechst 33342 can also be used to stain fixed cells by substituting Hoechst 33342 for DAPI[1].

    Molecular Weight




    CAS No.





    Room temperature in continental US; may vary elsewhere.


    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    Solvent & Solubility
    In Vitro: 

    DMSO : 10 mg/mL (22.10 mM; Need ultrasonic)

    H2O : < 0.1 mg/mL (insoluble)

    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.2097 mL 11.0485 mL 22.0970 mL
    5 mM 0.4419 mL 2.2097 mL 4.4194 mL
    10 mM 0.2210 mL 1.1049 mL 2.2097 mL
    *Please refer to the solubility information to select the appropriate solvent.
    Cell Assay

    Labeling Nuclear DNA with Hoechst 33342[1] Step 1, Dilute the Hoechst stock solution 1:100 in H2O for use in labeling. Step 2, Aspirate the cell medium from cells grown on coverslips. Rinse the cells three times with PBS+. Step 3, Incubate the cells in the Hoechst labeling solution (from Step 1) for 10-30 min at room temperature. Step 4, Aspirate the labeling solution. Rinse the cells three times in PBS+. Step 5, Mount the coverslips. Step 6, Image the cells (λex ~353 nm, λem ~483 nm for Hoechst 33342)[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

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