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  2. Fluorescein di(β-D-galactopyranoside)

Fluorescein di(β-D-galactopyranoside) (Synonyms: FDG)

Cat. No.: HY-101895 Purity: >98.0%
Handling Instructions

Fluorescein di(β-D-galactopyranoside) is a fluorogenic substrate for β-galactosidase (λex=485 nm, λem=535 nm).

For research use only. We do not sell to patients.

Fluorescein di(β-D-galactopyranoside) Chemical Structure

Fluorescein di(β-D-galactopyranoside) Chemical Structure

CAS No. : 17817-20-8

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Based on 1 publication(s) in Google Scholar

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Description

Fluorescein di(β-D-galactopyranoside) is a fluorogenic substrate for β-galactosidase (λex=485 nm, λem=535 nm).

In Vitro

The fluorescence produced by Fluorescein di(β-D-galactopyranoside) increases in a time- and dose-dependent manner. The level of fluorescence produced by the double-substrate method is much lower than that by the Fluorescein di(β-D-galactopyranoside) method. Results show that the fluorescein produced by Fluorescein di(β-D-galactopyranoside) in Hs68 cells is proportional to the number of passages[1].

Molecular Weight

656.59

Formula

C₃₂H₃₂O₁₅

CAS No.

17817-20-8

SMILES
Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
In solvent -80°C 6 months
  -20°C 1 month
References
Cell Assay
[1]

The cells (5×103 cells per well) are cultured in a 96-well plate overnight for attachment, washed, and then fixed in solutions. An aliquot (100 μL) of the reaction buffer (i.e., the staining solution without X-Gal) is added into each well. Then, 10 μL of 2 mM Fluorescein di(β-D-galactopyranoside) is added per well and the plate is incubated in the dark at 37°C for 24 h without CO2 supply. After incubation at 37°C for 24 h, 100 μL of the supernatant is transferred to a 96-well plate for fluorescent measurement in triplicates. The fluorescein fluorescence is measured using a fluorometer with an excitation at 485 nm and an emission at 535 nm[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

Purity: >98.0%

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Fluorescein di(β-D-galactopyranoside)
Cat. No.:
HY-101895
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