2. Academic Validation
  3. Ultrasensitive Analysis of Exosomes Using a 3D Self-Assembled Nanostructured SiO2 Microfluidic Chip

Ultrasensitive Analysis of Exosomes Using a 3D Self-Assembled Nanostructured SiO2 Microfluidic Chip

  • ACS Appl Mater Interfaces. 2022 Mar 30;14(12):14693-14702. doi: 10.1021/acsami.1c22569.
Qiaoyu Li 1,  Yanlin Wang 1,  Yuyan Xue 1,  Liang Qiao 1,  Guopeng Yu 2,  Yushan Liu 2,  Shaoning Yu 1 3
Affiliations

Affiliations

  • 1 Department of Chemistry, Fudan University, Shanghai 200438, China.
  • 2 Department of Urology, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200011, China.
  • 3 Zhejiang Provincial Key Laboratory of Advanced Mass Spectrometry and Molecular Analysis, Institute of Mass Spectrometry, School of Material Science and Chemical Engineering, Ningbo University, Ningbo, Zhejiang 315211, China.
PMID: 35199982 DOI: 10.1021/acsami.1c22569
Abstract

Conventional microfluidics with a solid mixer for exosome detection is constrained by the low binding efficiency of the solid-liquid boundary effects and reduced sensitivity of individual markers. Here, we report a 3D-SiO2 porous chip that combines nanoscale porous characteristics and multiple exosome specific markers to greatly improve the sensitivity for biosensing. The lower limit of detection was 220 particles/μL exosomes in PBS. We applied the 3D-SiO2 porous chip for prostate Cancer (PCa) staging in mice and early detection of clinical PCa patients. The developed method could significantly differentiate the different stages of PCa in mice and improve the early detection rate in clinical patients. Expression of multiple specific markers in clinical serum samples identified disease fingerprints, alongside histological results, which supports the potential application of exosomes as a noninvasive surrogate biopsy for PCa.

Keywords

3D image; 3D-SiO2 porous chip; microfluidic; multiple protein markers; prostate cancer.

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