BODIPY 505/515
Based on 3 publication(s) in Google Scholar
BODIPY505/515 is a BODIPY dye. BODIPY dye is a small molecule dye with strong ultraviolet absorption ability, its fluorescence peak is relatively sharp, and the quantum yield is high. They are relatively insensitive to the polarity and pH of the environment and are relatively stable under different physiological conditions. Due to its structural asymmetry, BODIPY derives a variety of structural products. BODIPY lipid droplet dyes can well pass through the cell membrane into the cell, and localize the neutral lipids in the cell to specifically stain the lipid droplets, which can be used for labeling of live cells and fixed cells. Maximum excitation/emission wavelength: 505/515 nm.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza : 99.94%
- No. CAS: 21658-70-8
- Fòrmula: C13H15BF2N2
- Peso molecular:248.08
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Almacenamiento:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Protocolo
- Pureza y Documentación
- Referencias
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Help & FAQs
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HY-W090090
BODIPY 493/503Neutral Lipid Dye -
HY-D0718
Nile RedHydrophobic Fluorescent Stain -
HY-DY1004
BODIPY 493/503 (solution)Neutral Lipid Dye -
HY-138226
BODIPY 558/568 C12Lipid Droplets Probe -
HY-10431
SB-431542TGF-β Receptor Kinase Inhibitor -
HY-13966
2-Deoxy-D-glucoseGlycolysis Inhibitor -
HY-10432
A 83-01ALK4/5/7 Inhibitor -
HY-15465
KN-93CaMK II Inhibitor -
HY-13704
SN-38Topoisomerase I Inhibitor -
HY-12305
Q-VD-OPhPan-caspase Inhibitor -
HY-D1055
MitoSOX RedMitochondrial ROS Indicator -
HY-101103
HP-β-CDCo-solvent
Publications Citing Use of MedChemExpress (MCE) BODIPY 505/515
More
Customer Validation & Images
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Cell Imaging/Staining
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Cell Imaging/Staining
Actividad biológica
Descripciòn
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HL-60 | IC50 |
>100 μM
Compound: 1
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Cytotoxicity against human HL60 cells after 24 hrs by MTT assay
Cytotoxicity against human HL60 cells after 24 hrs by MTT assay
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[PMID: 20199028] |
| HL-60 | IC50 |
4.4 μM
Compound: 1
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Phototoxicity against human HL60 cells irradiated with 4.1 J/cm^2 broad spectrum light treated 2 hrs before irradiation measured after 24 hrs by MTT assay
Phototoxicity against human HL60 cells irradiated with 4.1 J/cm^2 broad spectrum light treated 2 hrs before irradiation measured after 24 hrs by MTT assay
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[PMID: 20199028] |
| HSC-2 | IC50 |
>100 μM
Compound: 1
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Cytotoxicity against human HSC2 cells after 24 hrs by MTT assay
Cytotoxicity against human HSC2 cells after 24 hrs by MTT assay
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[PMID: 20199028] |
| HSC-2 | IC50 |
8.7 μM
Compound: 1
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Phototoxicity against human HSC2 cells irradiated with 4.1 J/cm^2 broad spectrum light treated 2 hrs before irradiation measured after 24 hrs by MTT assay
Phototoxicity against human HSC2 cells irradiated with 4.1 J/cm^2 broad spectrum light treated 2 hrs before irradiation measured after 24 hrs by MTT assay
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[PMID: 20199028] |
In Vitro
Guide (The following is our recommended solution. This solution is merely a guideline and should be modified according to your specific needs.)
1. Preparation of BODIPY505/515 working solution
1.1 Preparation of storage solution
Dilute 1 mg of BODIPY505/515 with 382 μL of anhydrous DMSO to prepare a 10 mM stock solution.
1.2 Preparation of working solution
Dilute the stored solution with preheated serum-free cell culture medium or PBS to prepare a 1-10 μM BODIPY505/515 working solution.
Note: Please adjust the concentration of the BODIPY505/515 working solution according to the actual situation, and prepare it as needed.
2. Cell Staining (Suspension Cells)
2.1 Centrifuge to collect the cells, then add PBS for two washes, each for 5 minutes. The cell density is 1×106/mL
2.2 Add 1 mL of BODIPY505/515 working solution and incubate at room temperature for 5 to 30 minutes.
2.3 400 g, centrifuge for 3-4 minutes, then discard the supernatant.
2.4 Wash the cells twice with PBS, each time for 5 minutes.
2.5 After resuspending the cells in 1 mL of serum-free medium or PBS, observe them using a fluorescence microscope or a flow cytometer.
3. Cell Staining (Monolayer Cells)
3.1 Place the monolayer cells on a sterile cover slip.
3.2 Remove the cover glass from the culture medium and aspirate away the excess medium.
3.3 Add 100 μL of the dye working solution and gently shake to ensure complete coverage of the cells. Incubate for 5 to 30 minutes.
3.4 Remove the dye working solution and wash with the culture medium 2-3 times, each time for 5 minutes. Observe using a fluorescence microscope or flow cytometer.
Notes
1. Please adjust the concentration of BODIPY505/515 working solution and the incubation time according to the actual situation.
2. It is recommended to conduct a positive control in the experiment. The cells in the control group should be incubated with 30 μM oleic acid for 8 hours before proceeding with the subsequent experiments.
3. This product is exclusively for professional scientific research purposes only. It shall not be used for clinical diagnosis or treatment, nor for food or medicine purposes.
4. For your safety and health, please wear a laboratory coat and put on disposable gloves when operating.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Emission (Em)
515
Excitation (Ex)
505
Chemical Information
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No. CAS 21658-70-8
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Appearance Solid
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Peso molecular 248.08
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Fòrmula C13H15BF2N2
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Color Brown to red
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SMILES
[F-][B+3]1([N]2=C(C)C=C(C)C2=CC3=C(C)C=C(C)[N-]31)[F-]
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (3)
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Journal Impact Factor
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Most Recent
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Int J Biol Macromol
Apolipoprotein E facilitates titanium implant osseointegration by regulating osteogenesis-lipogenesis balance. [Abstract]2023 May 1:236:123998. PMID: 36906203
BODIPY 505/515 purchased from MedChemExpress. Usage Cited in: Int J Biol Macromol. 2023 May 1:236:123998. [Abstract]
Representative images showed the immunofluorescence staining of BODIPY 505/515 in bone marrow mesenchymal stem cells (BMMSCs).
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BODIPY 505/515 purchased from MedChemExpress. Usage Cited in: Mater Design. 2023 Apr.
Lipid droplets (LDs) (BODIPY505/515) and Ce6 from pTP-Ce6-Apo were co-localized with the A375 nucleus by laser confocal microscope in A375 cells.
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Solvente y solubilidad
In Vitro:
DMSO : 12.5 mg/mL (50.39 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocolo
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Lipid Droplets: Oil Red O/Sudan Dye Lipid Staining
Lipid droplets are intracellular organelles with a neutral-lipid core that stores triacylglycerols and sterol esters, and Oil Red O or Sudan dyes detect these hydrophobic lipid deposits by partitioning into retained lipids in fresh or frozen specimens. Oil Red O stains neutral triglycerides and lipids in frozen tissue sections or air-dried cytologic preparations, while Sudan Black B has also been used as a histochemical fat stain for lipid-rich tissue structures.
Pureza y Documentación
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Ficha de datos (284 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Dong Xu, et al. Detection and quantitation of lipid in the microalga Tetraselmis subcordiformis (Wille) Butcher with BODIPY 505/515 staining. Bioresour Technol. 2013 Jan;127:386-90. [Content Brief]
[2]. Bo Qiu, et al. BODIPY 493/503 Staining of Neutral Lipid Droplets for Microscopy and Quantification by Flow Cytometry. Bio Protoc. 2016 Sep 5;6(17):e1912. [Content Brief]
[3]. Dong Xu, et al. Detection and quantitation of lipid in the microalga Tetraselmis subcordiformis (Wille) Butcher with BODIPY 505/515 staining. Bioresour Technol. 2013 Jan;127:386-90. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 4.0310 mL | 20.1548 mL | 40.3096 mL | 100.7739 mL |
| 5 mM | 0.8062 mL | 4.0310 mL | 8.0619 mL | 20.1548 mL | |
| 10 mM | 0.4031 mL | 2.0155 mL | 4.0310 mL | 10.0774 mL | |
| 15 mM | 0.2687 mL | 1.3437 mL | 2.6873 mL | 6.7183 mL | |
| 20 mM | 0.2015 mL | 1.0077 mL | 2.0155 mL | 5.0387 mL | |
| 25 mM | 0.1612 mL | 0.8062 mL | 1.6124 mL | 4.0310 mL | |
| 30 mM | 0.1344 mL | 0.6718 mL | 1.3437 mL | 3.3591 mL | |
| 40 mM | 0.1008 mL | 0.5039 mL | 1.0077 mL | 2.5193 mL | |
| 50 mM | 0.0806 mL | 0.4031 mL | 0.8062 mL | 2.0155 mL |
BODIPY 505/515 Related Classifications
HY-D1237 Related Classifications
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