BODIPY 505/515 

BODIPY505/515 is a BODIPY dye. BODIPY dye is a small molecule dye with strong ultraviolet absorption ability, its fluorescence peak is relatively sharp, and the quantum yield is high. They are relatively insensitive to the polarity and pH of the environment and are relatively stable under different physiological conditions. Due to its structural asymmetry, BODIPY derives a variety of structural products. BODIPY lipid droplet dyes can well pass through the cell membrane into the cell, and localize the neutral lipids in the cell to specifically stain the lipid droplets, which can be used for labeling of live cells and fixed cells^[1]. Maximum excitation/emission wavelength: 505/515 nm^[1].

Guide (The following is our recommended solution. This solution is merely a guideline and should be modified according to your specific needs.)
1. Preparation of BODIPY505/515 working solution
1.1 Preparation of storage solution
Dilute 1 mg of BODIPY505/515 with 382 μL of anhydrous DMSO to prepare a 10 mM stock solution.
1.2 Preparation of working solution
Dilute the stored solution with preheated serum-free cell culture medium or PBS to prepare a 1-10 μM BODIPY505/515 working solution.
Note: Please adjust the concentration of the BODIPY505/515 working solution according to the actual situation, and prepare it as needed.
2. Cell Staining (Suspension Cells)
2.1 Centrifuge to collect the cells, then add PBS for two washes, each for 5 minutes. The cell density is 1×10⁶/mL
2.2 Add 1 mL of BODIPY505/515 working solution and incubate at room temperature for 5 to 30 minutes.
2.3 400 g, centrifuge for 3-4 minutes, then discard the supernatant.
2.4 Wash the cells twice with PBS, each time for 5 minutes.
2.5 After resuspending the cells in 1 mL of serum-free medium or PBS, observe them using a fluorescence microscope or a flow cytometer.
3. Cell Staining (Monolayer Cells)
3.1 Place the monolayer cells on a sterile cover slip.
3.2 Remove the cover glass from the culture medium and aspirate away the excess medium.
3.3 Add 100 μL of the dye working solution and gently shake to ensure complete coverage of the cells. Incubate for 5 to 30 minutes.
3.4 Remove the dye working solution and wash with the culture medium 2-3 times, each time for 5 minutes. Observe using a fluorescence microscope or flow cytometer.

Notes
1. Please adjust the concentration of BODIPY505/515 working solution and the incubation time according to the actual situation.
2. It is recommended to conduct a positive control in the experiment. The cells in the control group should be incubated with 30 μM oleic acid for 8 hours before proceeding with the subsequent experiments.
3. This product is exclusively for professional scientific research purposes only. It shall not be used for clinical diagnosis or treatment, nor for food or medicine purposes.
4. For your safety and health, please wear a laboratory coat and put on disposable gloves when operating.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

248.08

C₁₃H₁₅BF₂N₂

21658-70-8

Solid

Brown to red

515

505

[F-][B+3]1([N]2=C(C)C=C(C)C2=CC3=C(C)C=C(C)[N-]31)[F-]

Room temperature in continental US; may vary elsewhere.

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

• [1]. Dong Xu, et al. Detection and quantitation of lipid in the microalga Tetraselmis subcordiformis (Wille) Butcher with BODIPY 505/515 staining. Bioresour Technol. 2013 Jan;127:386-90.  [Content Brief]

  [2]. Bo Qiu, et al. BODIPY 493/503 Staining of Neutral Lipid Droplets for Microscopy and Quantification by Flow Cytometry. Bio Protoc. 2016 Sep 5;6(17):e1912.  [Content Brief]

  [3]. Dong Xu, et al. Detection and quantitation of lipid in the microalga Tetraselmis subcordiformis (Wille) Butcher with BODIPY 505/515 staining. Bioresour Technol. 2013 Jan;127:386-90.  [Content Brief]