Fluorescein Diacetate
Based on 12 publication(s) in Google Scholar
Fluorescein diacetate is a cell permeable esterase-substrate. Fluorescein diacetate can be used as a fluorogenic substrate for hGSTP1-1.
For research use only. We do not sell to patients.
- Purity: 99.79%
- CAS No.: 596-09-8
- Formula: C24H16O7
- Molecular Weight:416.38
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Storage:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocol
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Fluorescein Diacetate
More- Adv Mater. 2025 Apr 29:e2418560. [Abstract]
- Biomaterials. 2025 May 5:322:123391. [Abstract]
- Chem Eng J. 2026 May 3;538:176959.
- Cell Death Discov. 2021 Dec 4;7(1):374. [Abstract]
- Collagen Leather. 2025 Nov 19，7(1):39.
- Int J Biol Macromol. 2024 Jun;269(Pt 2):131795. [Abstract]
- ACS Appl Mater Interfaces. 2023 Jun 28;15(25):29841-29853. [Abstract]
- Cell Prolif. 2024 Apr 16:e13646. [Abstract]
- Anal Chem. 2026 Mar 24;98(11):8133-8142. [Abstract]
- ACS Synth Biol. 2025 Sep 19;14(9):3767-3783. [Abstract]
- Toxicol Appl Pharmacol. 2026 Jul:512:117857. [Abstract]
- Toxicol Appl Pharmacol. 2025 Sep:502:117454. [Abstract]
Customer Validation & Images
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Cell Imaging/Staining
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Cell Imaging/Staining
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Cell Imaging/Staining
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Cell Imaging/Staining
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Cell Imaging/Staining
Biological Activity
Fluorescein diacetate (FDA) is an acetylated derivative of the green fluorescent dye fluorescein[1]. Fluorescein diacetate (FDA), a fluorescent probe used for vital staining, is a fluorescently activated by esterolytic activity of human Pi-class glutathione S-transferase (hGSTP1) selectively among various cytosolic GSTs. Fluorescence activation of Fluorescein diacetate susceptible to GST inhibitors is observed in MCF7 cells exogenously overexpressing hGSTP1, but not in cells overexpressing hGSTA1 or hGSTM1. Fluorescein diacetate can be used as a fluorogenic substrate for hGSTP1-1. To investigate whether the fluorescence activation is due to hGSTP1 activity, Fluorescein diacetate is incubated with recombinant hGSTP1-1 and GSH in vitro. Remarkable fluorescence activation is observed in the presence of both hGSTP1-1 and GSH, whereas only slight activation is observed in the absence of either of them or when the enzyme is heat inactivated. This suggests that the fluorescence activation of Fluorescein diacetate depends on hGSTP1-1 activity. From the linear relationship between the rate of increase in fluorescence and the hGSTP1-1 concentration, the specific activity of the enzyme for 1 μM Fluorescein diacetate is determined to be 79±15 nmol/min/mg protein. Fluorescein diacetate is applicable as a fluorogenic substrate for evaluating inhibitors of GSTP1-1 in vitro. For Fluorescein diacetate as a substrate, both Ethacrynic acid (EA) and NBDHEX suppress the hGSTP1-1-dependent fluorescent increase in a concentration-dependent manner, with IC50s of 3.3±0.5 μM and 0.61±0.04 μM, respectively[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 596-09-8
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Appearance Solid
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Molecular Weight 416.38
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Formula C24H16O7
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Color Light yellow to yellow
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SMILES
O=C1OC2(C3=C(OC4=C2C=CC(OC(C)=O)=C4)C=C(OC(C)=O)C=C3)C5=C1C=CC=C5
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Synonyms
3,6-Diacetoxyfluoran; Di-O-acetylfluorescein
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (12)
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Journal Impact Factor
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Most Recent
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Adv Mater
Hybrid Lipoplex Boosts Neuron-Microglia Crosstalk for Treatment of Alzheimer's Disease through Aβ-Targeted-Autophagy and ApoE2 Gene Supplementation. [Abstract]2025 Apr 29:e2418560. PMID: 40296752
Fluorescein Diacetate purchased from MedChemExpress. Usage Cited in: Adv Mater. 2025 Apr 29:e2418560. [Abstract]
The Fluorescein Diacetate (FDA; 100 μM; 20 min) staining images and brightfield (BF) images of SH-SY5Y cells, with validation of neuronal axonal growth repair by RMC/pApoE2 after co-culturing.
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Biomaterials
Radiation-triggerable bioreactors enable bioenergetic reprograming of cancer stem cell plasticity via targeted arginine metabolism disruption for augmented radio-immunotherapy. [Abstract]2025 May 5:322:123391. PMID: 40344881 -
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Cell Death Discov
Berberine alleviates liver fibrosis through inducing ferrous redox to activate ROS-mediated hepatic stellate cells ferroptosis. [Abstract]2021 Dec 4;7(1):374. PMID: 34864819
Fluorescein Diacetate purchased from MedChemExpress. Usage Cited in: Cell Death Discov. 2021 Dec 4;7(1):374. [Abstract]
Live cells and dead HSC-LX2 cells were assayed by Fluorescein Diacetate (FDA) staining and PI staining, respectively.
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Fluorescein Diacetate purchased from MedChemExpress. Usage Cited in: Collagen Leather. 2025 Nov 19，7(1):39.
Representative images (CLSM) of Fluorescein Diacetate (FDA)/PI staining of MSCs in different hydrogels on day 1, 3, and 7 in vitro culture.
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Int J Biol Macromol
A synergistically antimicrobial and antioxidant hyaluronic acid hydrogel for infected wounds. [Abstract]2024 Jun;269(Pt 2):131795. PMID: 38670175 -
ACS Appl Mater Interfaces
Wound-Healing Material with Antibacterial and Antioxidant Functions, Constructed Using Keratin, Hyperbranched Polymers, and MnO2. [Abstract]2023 Jun 28;15(25):29841-29853. PMID: 37338013 -
Cell Prolif
Deletion of Tgm2 suppresses BMP-mediated hepatocyte-to-cholangiocyte metaplasia in ductular reaction. [Abstract]2024 Apr 16:e13646. PMID: 38623945
Fluorescein Diacetate purchased from MedChemExpress. Usage Cited in: Cell Prolif. 2024 Apr 16:e13646. [Abstract]
Bright-field and Fluorescein Diacetate (FDA) fluorescence (green) images of single MCF-7 (top) and CT-26 (bottom) cells before and 2 h after the first sampling. Yellow arrows indicate the same cell before and after sampling; black shadows denote the nanocapillary.
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Anal Chem
Minimally Invasive Single-Cell Metabolomics Analysis to Unveil Different Trajectories of Individual Cells in Ferroptosis. [Abstract]2026 Mar 24;98(11):8133-8142. PMID: 41814887 -
ACS Synth Biol
Signal Peptide-Guided Delivery of a Mucin-Like Collagen Analogue for Periplasmic Barrier Reinforcement: A Platform for Enhancing Microbial Survival. [Abstract]2025 Sep 19;14(9):3767-3783. PMID: 40924575 -
Toxicol Appl Pharmacol
2026 Jul:512:117857. PMID: 42107544 -
Toxicol Appl Pharmacol
2025 Sep:502:117454. PMID: 40588104
Fluorescein Diacetate purchased from MedChemExpress. Usage Cited in: Toxicol Appl Pharmacol. 2025 Sep:502:117454. [Abstract]
Fluorescence images of the inhibitory effect of Carbofuran on CES2 in HepG2 cells with Fluorescein Diacetate (FD; 10 μM; 30 min). (a) 0 μM; (b) 5 μM; (c) 50 μM; (d) 100 μM;
Solvent & Solubility
DMSO : 23.33 mg/mL (56.03 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocol
MCF7 cells (2-3×105) are seeded in a 35-mm glass bottom dish before the experiment. Prior to imaging, cells are washed with 1 mL PBS, then incubated in 1 mL Hanks’ Balanced Salt solution (HBSS(+) without phenol red) containing 1 μM Fluorescein diacetate (0.1% DMSO as a cosolvent) for 5 min at 37°C. Cells are washed twice with 1 mL PBS and 1 mL HBSS is added before imaging. Fluorescence images of fluorescein and DsRed-Express2 were acquired in the FITC channel (excitation at 473 nm) and the DsRed channel (excitation 559 nm). The 16-bit images obtained are analysed[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (278 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Boyd V, et al. Limitations in the Use of Fluorescein Diacetate/Propidium Iodide (FDA/PI) and Cell PermeableNucleic Acid Stains for Viability Measurements of Isolated Islets of Langerhans. Curr Trends Biotechnol Pharm. 2008 Mar;2(2):66-84. [Content Brief]
[2]. Fujikawa Y, et al. Fluorescein diacetate (FDA) and its analogue as substrates for Pi-class glutathione S-transferase (GSTP1) and their biological application. Talanta. 2018 Mar 1;179:845-852. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 2.4017 mL | 12.0083 mL | 24.0165 mL | 60.0413 mL |
| 5 mM | 0.4803 mL | 2.4017 mL | 4.8033 mL | 12.0083 mL | |
| 10 mM | 0.2402 mL | 1.2008 mL | 2.4017 mL | 6.0041 mL | |
| 15 mM | 0.1601 mL | 0.8006 mL | 1.6011 mL | 4.0028 mL | |
| 20 mM | 0.1201 mL | 0.6004 mL | 1.2008 mL | 3.0021 mL | |
| 25 mM | 0.0961 mL | 0.4803 mL | 0.9607 mL | 2.4017 mL | |
| 30 mM | 0.0801 mL | 0.4003 mL | 0.8006 mL | 2.0014 mL | |
| 40 mM | 0.0600 mL | 0.3002 mL | 0.6004 mL | 1.5010 mL | |
| 50 mM | 0.0480 mL | 0.2402 mL | 0.4803 mL | 1.2008 mL |
Fluorescein Diacetate Related Classifications
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