Z-Gly-Pro-AMC
Based on 4 publication(s) in Google Scholar
Z-Gly-Pro-AMC is a fluorogenic substrate. Z-Gly-Pro-AMC is hydrolyzed by prolyl endopeptidase to generate highly fluorescent 7-Amino-4-methylcoumarin (HY-D0027). (λex=380 nm, λem=465 nm).
For research use only. We do not sell to patients.
- Purity: 99.81%
- CAS No.: 68542-93-8
- Formula: C25H25N3O6
- Molecular Weight:463.48
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Storage:
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Z-Gly-Pro-AMC
More
Biological Activity
Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Z-Gly-Pro-AMC assay[1]:
1. Make a 5 μL of plasma sample is pre-incubated with 10 μL of 250 nM FAP inhibitor, 10 μL of 250 nM PREP inhibitor or 10 μL of 0.0025% (v/v) DMSO for 15 min at 37 °C.
2. Add 35 μL pre-heated Z-Gly-Pro-AMC (380 μM diluted in buffer) to obtain a final concentration of 266 μM.
3. Fluorescence is measured kinetically for 30 min at 37 °C.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
460
380
Chemical Information
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CAS No. 68542-93-8
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Appearance Solid
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Molecular Weight 463.48
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Formula C25H25N3O6
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Color White to off-white
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SMILES
CC(C1=CC=C(NC([C@H]2N(CCC2)C(CNC(OCC3=CC=CC=C3)=O)=O)=O)C=C1O4)=CC4=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Publications (4)
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Journal Impact Factor
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Most Recent
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Adv Sci (Weinh)
2026 Mar 24:e23986. PMID: 41875349 -
J Control Release
Structure-guided design and clinical evaluation of 68Ga-GP01 for FAP-targeted PET imaging in solid tumors. [Abstract]2026 Jan 10:389:114475. PMID: 41325906 -
J Med Chem
A Small-Molecule Drug Conjugate Targeting FAP Exhibits Potent Activity in Dogs with Spontaneous Tumors. [Abstract]2026 May 28;69(10):12634-12646. PMID: 42142054 -
Bioconjug Chem
2025 Oct 28. PMID: 41147981
Solvent & Solubility
DMSO : 125 mg/mL (269.70 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (279 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.1576 mL | 10.7880 mL | 21.5759 mL | 53.9398 mL |
| 5 mM | 0.4315 mL | 2.1576 mL | 4.3152 mL | 10.7880 mL | |
| 10 mM | 0.2158 mL | 1.0788 mL | 2.1576 mL | 5.3940 mL | |
| 15 mM | 0.1438 mL | 0.7192 mL | 1.4384 mL | 3.5960 mL | |
| 20 mM | 0.1079 mL | 0.5394 mL | 1.0788 mL | 2.6970 mL | |
| 25 mM | 0.0863 mL | 0.4315 mL | 0.8630 mL | 2.1576 mL | |
| 30 mM | 0.0719 mL | 0.3596 mL | 0.7192 mL | 1.7980 mL | |
| 40 mM | 0.0539 mL | 0.2697 mL | 0.5394 mL | 1.3485 mL | |
| 50 mM | 0.0432 mL | 0.2158 mL | 0.4315 mL | 1.0788 mL | |
| 60 mM | 0.0360 mL | 0.1798 mL | 0.3596 mL | 0.8990 mL | |
| 80 mM | 0.0270 mL | 0.1348 mL | 0.2697 mL | 0.6742 mL | |
| 100 mM | 0.0216 mL | 0.1079 mL | 0.2158 mL | 0.5394 mL |
Z-Gly-Pro-AMC Related Classifications
HY-D1670 Related Classifications
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