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Art. -Nr.: HY-D1670

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Z-Gly-Pro-AMC

Name: Z-Gly-Pro-AMC
Brand: MedChemExpress
SKU: HY-D1670
Price: 35.00 USD
Availability: InStock
Rating: 5 (4 reviews)

4 Cited Publications

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Based on 4 publication(s) in Google Scholar

Z-Gly-Pro-AMC is a fluorogenic substrate. Z-Gly-Pro-AMC is hydrolyzed by prolyl endopeptidase to generate highly fluorescent 7-Amino-4-methylcoumarin (HY-D0027). (λ_ex=380 nm, λ_em=465 nm).

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Reinheit : 99.81%
CAS. Nr.: 68542-93-8
Formel: C₂₅H₂₅N₃O₆
Molecular Weight:463.48

Speicherung:

-20°C, protect from light, stored under nitrogen

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Z-Gly-Pro-AMC Chemical Structure

CAS. Nr.: 68542-93-8

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- In Vitro
- Emission (Em)
- Excitation (Ex)
Chemical Information
Publications (4)
Lösungsmittel & Löslichkeit
- In Vitro
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Z-Gly-Pro-AMC is a fluorogenic substrate. Z-Gly-Pro-AMC is hydrolyzed by prolyl endopeptidase to generate highly fluorescent 7-Amino-4-methylcoumarin (HY-D0027). (λ_ex=380 nm, λ_em=465 nm)^[1].

In Vitro

Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Z-Gly-Pro-AMC assay^[1]:
1. Make a 5 μL of plasma sample is pre-incubated with 10 μL of 250 nM FAP inhibitor, 10 μL of 250 nM PREP inhibitor or 10 μL of 0.0025% (v/v) DMSO for 15 min at 37 °C.
2. Add 35 μL pre-heated Z-Gly-Pro-AMC (380 μM diluted in buffer) to obtain a final concentration of 266 μM.
3. Fluorescence is measured kinetically for 30 min at 37 °C.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Emission (Em)

460

Excitation (Ex)

380

Chemical Information

CAS. Nr. 68542-93-8
Appearance Solid
Molecular Weight 463.48
Formel C₂₅H₂₅N₃O₆
Color White to off-white
SMILES

CC(C1=CC=C(NC([C@H]2N(CCC2)C(CNC(OCC3=CC=CC=C3)=O)=O)=O)C=C1O4)=CC4=O
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

-20°C, protect from light, stored under nitrogen

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Publications (4)

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Journal Impact Factor

Adv Sci (Weinh)

Erasable FAP Targeted Spray-On Probe for Fluorescence-Guided Surgery. [Abstract]

2026 Mar 24:e23986. PMID: 41875349
J Control Release

Structure-guided design and clinical evaluation of 68Ga-GP01 for FAP-targeted PET imaging in solid tumors. [Abstract]

2026 Jan 10:389:114475. PMID: 41325906
J Med Chem

A Small-Molecule Drug Conjugate Targeting FAP Exhibits Potent Activity in Dogs with Spontaneous Tumors. [Abstract]

2026 May 28;69(10):12634-12646. PMID: 42142054
Bioconjug Chem

Far-Red Spray-On Imaging Probes for FAP-Targeted Cancer Surgery. [Abstract]

2025 Oct 28. PMID: 41147981

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 125 mg/mL (269.70 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.1576 mL

10.7880 mL

21.5759 mL

5 mM

0.4315 mL

2.1576 mL

4.3152 mL

10 mM

0.2158 mL

1.0788 mL

2.1576 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (279 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Bracke A, et, al. The development and validation of a combined kinetic fluorometric activity assay for fibroblast activation protein alpha and prolyl oligopeptidase in plasma. Clin Chim Acta. 2019 Aug;495:154-160. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.1576 mL	10.7880 mL	21.5759 mL	53.9398 mL
	5 mM	0.4315 mL	2.1576 mL	4.3152 mL	10.7880 mL
	10 mM	0.2158 mL	1.0788 mL	2.1576 mL	5.3940 mL
	15 mM	0.1438 mL	0.7192 mL	1.4384 mL	3.5960 mL
	20 mM	0.1079 mL	0.5394 mL	1.0788 mL	2.6970 mL
	25 mM	0.0863 mL	0.4315 mL	0.8630 mL	2.1576 mL
	30 mM	0.0719 mL	0.3596 mL	0.7192 mL	1.7980 mL
	40 mM	0.0539 mL	0.2697 mL	0.5394 mL	1.3485 mL
	50 mM	0.0432 mL	0.2158 mL	0.4315 mL	1.0788 mL
	60 mM	0.0360 mL	0.1798 mL	0.3596 mL	0.8990 mL
	80 mM	0.0270 mL	0.1348 mL	0.2697 mL	0.6742 mL
	100 mM	0.0216 mL	0.1079 mL	0.2158 mL	0.5394 mL

Bioz

Keywords

Z-Gly-Pro-AMC

4 Cited Publications

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Biologische Aktivität
- Beschreibung
- In Vitro
- Emission (Em)
- Excitation (Ex)
Chemical Information
Publications (4)
Lösungsmittel & Löslichkeit
- In Vitro
Reinheit & Dokumentation
Verweise

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Z-Gly-Pro-AMC

Publications Citing Use of MedChemExpress (MCE) Z-Gly-Pro-AMC