Z-Arg-Arg-AMC hydrochloride
Based on 2 publication(s) in Google Scholar
Z-Arg-Arg-AMC hydrochloride is a highly selective fluorescent Cathepsin B substrate. Z-Arg-Arg-AMC hydrochloride can be hydrolyzed by Cathepsin B to produce a fluorescent product for enzyme activity detection.
For research use only. We do not sell to patients.
- Purity: 99.87%
- CAS No.: 136132-67-7
- Formula: C30H40ClN9O6
- Molecular Weight:658.15
-
Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
-
HY-D0027
7-Amino-4-methylcoumarin
-
HY-P4515
Z-Phe-Arg-pNA
-
HY-D0843
N-Ethylmaleimide
-
HY-148905
p-Aminophenylmercuric acetate
-
HY-18234A
Leupeptin hemisulfate
-
HY-100350
CA-074 methyl ester
-
HY-P1002
Suc-Leu-Leu-Val-Tyr-AMC
-
HY-15533
LY 3000328
-
HY-10042
Odanacatib
-
HY-10162
Olaparib
-
HY-15282
E-64
-
HY-123053
Z-Leu-Leu-Glu-AMC
Publications Citing Use of MedChemExpress (MCE) Z-Arg-Arg-AMC hydrochloride
MoreAll Cathepsin Isoforms
More
Biological Activity
|
Cathepsin B |
Guidelines (The following is our recommended protocol, which serves only as a guideline and should be modified according to your specific needs)[1].
1. Preparation of Working Solutions:
Prepare a 60 μM Z-Arg-Arg-AMC hydrochloride solution.
2. Specific Procedures:
1) Prepare a 1:3000 diluted working solution of human cathepsin B in periplasmic analysis buffer (PAB; sodium bicarbonate-free, containing 0.6 mM CaCl2, 0.6 mM MgCl2, 2 mM L-cysteine, 25 mM Pipes, adjusted to pH 7.0), corresponding to an enzyme concentration of 225 ng/mL with a specific activity of 588 U/mg.
2) The enzymatic reaction system has a volume of 0.2 mL and is performed in a 96-well plate. The final enzyme dilution ratio is 1:30000 (22.5 ng/mL; 0.9 mM), with 60 μM Z-Arg-Arg-AMC as the substrate.
3) Inhibitors and their dilutions are prepared with dimethyl sulfoxide. The reaction system of the untreated group receives an equal volume of dimethyl sulfoxide containing excipients (final concentration 0.1% v/v). A Fmax fluorescence microplate reader set to kinetic mode with the accompanying SoftPro software (Molecular Devices, Sunnyvale, CA, USA) measures the reaction rate (relative fluorescence units per minute), with an excitation wavelength of 355 nm and an emission wavelength of 460 nm. The reaction proceeds at 37 ℃ for 10 min, with data read every 30 s.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
CAS No. 136132-67-7
-
Appearance Solid
-
Molecular Weight 658.15
-
Formula C30H40ClN9O6
-
Color White to off-white
-
Sequence
Z-Arg-Arg-AMC
-
Sequence Shortening
Z-RR-AMC
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (2)
-
Journal Impact Factor
-
Most Recent
-
J Med Chem
Discovery of Thiazole Carboxamides as Novel Vanin-1 Inhibitors for Inflammatory Bowel Disease Treatment. [Abstract]2024 Nov 28;67(22):20372-20398. PMID: 39514323 -
Pharm Res
Rational Design, Optimization and Bioinformatic Analysis of Anti-PD-L1 Peptide Conjugated Mocetinostat Prodrug Nanoparticles for Predictive Cancer Chemoimmunotherapeutic Efficacy. [Abstract]2026 May 27. PMID: 42204129
Solvent & Solubility
DMSO : 100 mg/mL (151.94 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (3.80 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (3.80 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
-
Data Sheet (276 KB)
-
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
-
Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.5194 mL | 7.5971 mL | 15.1941 mL | 37.9853 mL |
| 5 mM | 0.3039 mL | 1.5194 mL | 3.0388 mL | 7.5971 mL | |
| 10 mM | 0.1519 mL | 0.7597 mL | 1.5194 mL | 3.7985 mL | |
| 15 mM | 0.1013 mL | 0.5065 mL | 1.0129 mL | 2.5324 mL | |
| 20 mM | 0.0760 mL | 0.3799 mL | 0.7597 mL | 1.8993 mL | |
| 25 mM | 0.0608 mL | 0.3039 mL | 0.6078 mL | 1.5194 mL | |
| 30 mM | 0.0506 mL | 0.2532 mL | 0.5065 mL | 1.2662 mL | |
| 40 mM | 0.0380 mL | 0.1899 mL | 0.3799 mL | 0.9496 mL | |
| 50 mM | 0.0304 mL | 0.1519 mL | 0.3039 mL | 0.7597 mL | |
| 60 mM | 0.0253 mL | 0.1266 mL | 0.2532 mL | 0.6331 mL | |
| 80 mM | 0.0190 mL | 0.0950 mL | 0.1899 mL | 0.4748 mL | |
| 100 mM | 0.0152 mL | 0.0760 mL | 0.1519 mL | 0.3799 mL |
Z-Arg-Arg-AMC hydrochloride Related Classifications
HY-134434 Related Classifications
Powered by Bioz