2. Peptides
  3. Peptide and Derivatives
  4. Inhibitors and Substrates
  5. Enzyme Substrates

Enzyme Substrates

Custom Peptide Synthesis

Enzyme substrate peptides are short peptides containing specific recognition sequences for target enzymes. When modified or cleaved by these enzymes, they generate detectable reporter signals such as fluorescence, thereby enabling the study of enzyme activity and facilitating applications in drug screening.

Enzyme Substrates (644):

Cat. No. Product Name CAS No. Purity Chemical Structure
  • HY-P1002
    Suc-Leu-Leu-Val-Tyr-AMC 94367-21-2 99.66%
    Suc-Leu-Leu-Val-Tyr-AMC is a membrane-permeable calpain-specific fluorogenic substrate (Ex/Em = 390/480 nm).
    Suc-Leu-Leu-Val-Tyr-AMC
  • HY-134124
    Glutathione ethyl ester 92614-59-0 98.23%
    Glutathione ethyl ester is a cell-permeable GSH donor and provides an efficient supply of GSH to the oocyte. Glutathione ethyl ester shows positive effect on the in vitro production of embryos by enhancement of the antioxidative defense.
    Glutathione ethyl ester
  • HY-P2573
    Suc-AAPF-pNA 70967-97-4 99.55%
    Suc-AAPF-pNA (Suc-Ala-Ala-Pro-Phe-pNA) is a chromogenic p-nitroanilide (pNA) substrate with the Km of 1.7 mM. Cleavage of Suc-AAPF-pNA releases 4-nitroaniline, which is yellow in colour and can be measured spectrophotometrically. Suc-AAPF-pNA can be used for the measurement of free and membrane-bound cathepsin G in human neutrophils.
    Suc-AAPF-pNA
  • HY-123053
    Z-Leu-Leu-Glu-AMC 348086-66-8 99.46%
    Z-Leu-Leu-Glu-AMC (Z-LLE-AMC) is a peptide-AMC linked substrate used to measure the postacidic-like hydrolysing activity of proteasome. Z-Leu-Leu-Glu-AMC can be used for the research of parkinson's disease.
    Z-Leu-Leu-Glu-AMC
  • HY-W010991
    N-[3-(2-Furyl)acryloyl]-Phe-Gly-Gly 64967-39-1 99.81%
    N-[3-(2-Furyl)acryloyl]-Phe-Gly-Gly (FAPGG) is a specific substrate of angiotensin converting enzyme (ACE) with a Ki of 2.546×10-4 M. It is used as a chromogenic probe for quantitative detection of ACE activity. N-[3-(2-Furyl)acryloyl]-Phe-Gly-Gly can be hydrolyzed by ACE to generate N-[3-(2-furyl)acryloyl]-Phe (FAP) and Gly-Gly, and the ACE inhibitory effect is monitored by photometry. FAPGG competitively binds to the active center of ACE and is a key tool for screening ACE inhibitors such as Captopril (HY-B0368) and Dioscorin. Its reversible mechanism of action supports hypertension research and drug development targeting the renin-angiotensin system.
    N-[3-(2-Furyl)acryloyl]-Phe-Gly-Gly
  • HY-P5450A
    PDKtide TFA 99.07%
    PDKtide TFA, a biological active peptide, is a substrate for phosphatidylinositide-dependent kinase 1 (PDK1).
    PDKtide TFA
  • HY-P10541A
    HSP70/DnaK substrate peptide TFA 98.06%
    HSP70/DnaK substrate peptide TFA is a short peptide that the HSP70/DnaK molecular chaperone can bind and act on. HSP70/DnaK substrate peptide TFA can be used to study the mechanism of action of HSP70/DnaK in molecular chaperone function.
    HSP70/DnaK substrate peptide TFA
  • HY-P4787A
    Ac-Glu-Asp(EDANS)-Lys-Pro-Ile-Leu-Phe-Phe-Arg-Leu-Gly-Lys(DABCYL)-Glu-NH2 acetate 99.44%
    Ac-Glu-Asp(EDANS)-Lys-Pro-Ile-Leu-Phe-Phe-Arg-Leu-Gly-Lys(DABCYL)-Glu-NH2 acetate is a Cathepsin D substrate, that can be used for cathepsin D FRET assay.
    Ac-Glu-Asp(EDANS)-Lys-Pro-Ile-Leu-Phe-Phe-Arg-Leu-Gly-Lys(DABCYL)-Glu-NH2 acetate
  • HY-P4931
    Mca-KPLGL-Dap(Dnp)-AR-NH2 720710-69-0 99.72%
    Mca-Lys-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2 (FS-6) is a fluorescent peptide that is a quenched MMP peptide substrate. Mca-Lys-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2 can be used for real-time quantification of MMP enzymatic activity. Mca-Lys-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2 is an elongated peptide of MMP substrate (FS-1) and is active against collagenases (MMP-1, MMP-8, MMP-13 ) and MT1-MMP with higher specificity constants than FS-1. (Ex/Em=325 nm/400 nm)
    Mca-KPLGL-Dap(Dnp)-AR-NH2
  • HY-136626
    Z-Ala-Ala-Asn-AMC 149697-16-5 99.28%
    Z-Ala-Ala-Asn-AMC (Cbz-Ala-Ala-Asn-AMC) is the legumain substrate. Overexpressed legumain in 293 HEK-Leg cells potently cleaved CBZ-Ala-Ala-Asn-AMC.
    Z-Ala-Ala-Asn-AMC
  • HY-134434
    Z-Arg-Arg-AMC hydrochloride 136132-67-7 99.83%
    Z-Arg-Arg-AMC hydrochloride is a highly selective fluorescent Cathepsin B substrate. Z-Arg-Arg-AMC hydrochloride can be hydrolyzed by Cathepsin B to produce a fluorescent product for enzyme activity detection.
    Z-Arg-Arg-AMC hydrochloride
  • HY-P0019
    Z-Gly-Gly-Arg-AMC 66216-78-2 99.98%
    Z-Gly-Gly-Arg-AMC is a thrombin-specific fluorogenic substrate for testing of thrombin generation in PRP and platelet-poor plasma (PPP) (Ex/Em = 390/480 nm).
    Z-Gly-Gly-Arg-AMC
  • HY-136865
    MeOSuc-AAPV-AMC 72252-90-5 98.43%
    MeOSuc-AAPV-AMC is a fluorogenic substrate for human leukocyte and porcine pancreatic elastase (Km: 362 μM, Ex=380 nm, Em=460 nm).
    MeOSuc-AAPV-AMC
  • HY-P1003
    Ac-DEVD-AMC 169332-61-0 99.18%
    Ac-DEVD-AMC is a fluorescent substrate of caspase-3/caspase-7. When treating Ac-DEVD-AMC with cell lysate, Ac-DEVD-AMC releases amino-4-methylcoumarin (AMC) for fluorescence detection, with an excitation wavelength of 380 nm and an emission wavelength of 460 nm.
    Ac-DEVD-AMC
  • HY-P0019A
    Z-Gly-Gly-Arg-AMC acetate 2070009-61-7 99.84%
    Z-Gly-Gly-Arg-AMC acetate is a thrombin-specific fluorogenic substrate for testing of thrombin generation in PRP and platelet-poor plasma (PPP) (Ex/Em = 390/480 nm).
    Z-Gly-Gly-Arg-AMC acetate
  • HY-P1005
    Ac-DEVD-AFC 201608-14-2 98.89%
    Ac-DEVD-AFC is a fluorogenic substrate (λex=400 nm, λem=530 nm).
    Ac-DEVD-AFC
  • HY-P2660
    Gly-Asp-Asp-Asp-Asp-Lys-β-naphthylamide 70023-02-8 99.95%
    Gly-Asp-Asp-Asp-Asp-Lys-β-naphthylamide (GD4K-na) is a substrate for human enteropeptidase (Km: 0.16 mM).
    Gly-Asp-Asp-Asp-Asp-Lys-β-naphthylamide
  • HY-134432B
    Boc-Gln-Ala-Arg-AMC acetate 99.99%
    Boc-Gln-Ala-Arg-AMC acetate is a fluorogenic substrate for trypsin. Boc-Gln-Ala-Arg-AMC acetate can also be used for measuring the proteolytic activity of TMPRSS2.
    Boc-Gln-Ala-Arg-AMC acetate
  • HY-P2295
    Dabcyl-KTSAVLQSGFRKME-Edans TFA 99.75%
    Dabcyl-KTSAVLQSGFRKME-Edans TFA is a fluorogenic peptide. Dabcyl-KTSAVLQSGFRKME-Edans TFA is used as the substrate to measure the enzymatic activities of protease forms. Dabcyl-KTSAVLQSGFRKME-Edans TFA has the potential for study 2019-nCoV (COVID-19) infection.
    Dabcyl-KTSAVLQSGFRKME-Edans TFA
  • HY-P4217
    Z-Gly-Pro-Arg-AMC hydrochloride 201928-42-9 99.15%
    Z-Gly-Pro-Arg-AMC hydrochloride a fluorescent trypsin and cathepsin K substrate. Z-Gly-Pro-Arg-AMC hydrochloride can be used to determine trypsin and cathepsin K activity.
    Z-Gly-Pro-Arg-AMC hydrochloride