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Z-Ala-Ala-Asn-AMC (Synonyms: Cbz-Ala-Ala-Asn-AMC)

Cat. No.: HY-136626 Purity: 98.82%
Handling Instructions

Z-Ala-Ala-Asn-AMC (Cbz-Ala-Ala-Asn-AMC) is the legumain substrate. Overexpressed legumain in 293 HEK-Leg cells potently cleaved CBZ-Ala-Ala-Asn-AMC.

For research use only. We do not sell to patients.

Z-Ala-Ala-Asn-AMC Chemical Structure

Z-Ala-Ala-Asn-AMC Chemical Structure

CAS No. : 149697-16-5

Size Price Stock Quantity
Solution
10 mM * 1 mL in DMSO USD 423 In-stock
Estimated Time of Arrival: December 31
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
USD 423 In-stock
Estimated Time of Arrival: December 31
Solid
1 mg USD 140 In-stock
Estimated Time of Arrival: December 31
5 mg USD 340 In-stock
Estimated Time of Arrival: December 31
10 mg USD 630 In-stock
Estimated Time of Arrival: December 31
50 mg   Get quote  
100 mg   Get quote  

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Based on 1 publication(s) in Google Scholar

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Description

Z-Ala-Ala-Asn-AMC (Cbz-Ala-Ala-Asn-AMC) is the legumain substrate. Overexpressed legumain in 293 HEK-Leg cells potently cleaved CBZ-Ala-Ala-Asn-AMC[1].

In Vitro

The first legumain fluorogenic substrate Cbz-Ala-Ala-Asn-AMC based on the P3-P2-P1 sequence Ala-Ala-Asn. The synthetic substrate Cbz-Ala-Ala-Asn-AMC is effectively cleaved by S. mansoni legumain and human legumain with Kms of 90 and 80 uM respectively[2][3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

565.57

Formula

C₂₈H₃₁N₅O₈

CAS No.
SMILES

O=C(N)C[[email protected]@H](C(NC1=CC=C(C(O2)=C1)C(C)=CC2=O)=O)NC([[email protected]](C)NC([[email protected]](C)NC(OCC3=CC=CC=C3)=O)=O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -80°C 2 years
-20°C 1 year
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (88.41 mM; Need ultrasonic)

H2O : < 0.1 mg/mL (ultrasonic) (insoluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.7681 mL 8.8406 mL 17.6813 mL
5 mM 0.3536 mL 1.7681 mL 3.5363 mL
10 mM 0.1768 mL 0.8841 mL 1.7681 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (4.42 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (4.42 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (4.42 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
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Keywords:

Z-Ala-Ala-Asn-AMCCbz-Ala-Ala-Asn-AMCOtherslegumainsubstrateInhibitorinhibitorinhibit

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Product Name:
Z-Ala-Ala-Asn-AMC
Cat. No.:
HY-136626
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