Z-Ala-Ala-Asn-AMC
Based on 3 publication(s) in Google Scholar
Z-Ala-Ala-Asn-AMC (Cbz-Ala-Ala-Asn-AMC) is the legumain substrate. Overexpressed legumain in 293 HEK-Leg cells potently cleaved CBZ-Ala-Ala-Asn-AMC.
For research use only. We do not sell to patients.
- Purity: 99.28%
- CAS No.: 149697-16-5
- Formula: C28H31N5O8
- Molecular Weight:565.57
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Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Z-Ala-Ala-Asn-AMC
More
Biological Activity
The first legumain fluorogenic substrate Cbz-Ala-Ala-Asn-AMC based on the P3-P2-P1 sequence Ala-Ala-Asn. The synthetic substrate Cbz-Ala-Ala-Asn-AMC is effectively cleaved by S. mansoni legumain and human legumain with Kms of 90 and 80 uM respectively[2][3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 149697-16-5
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Appearance Solid
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Molecular Weight 565.57
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Formula C28H31N5O8
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Color White to off-white
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Synonyms
Cbz-Ala-Ala-Asn-AMC
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Sequence
Cbz-Ala-Ala-Asn-AMC
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Sequence Shortening
Cbz-AAN-AMC
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (3)
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Journal Impact Factor
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Most Recent
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Cell Death Dis
Legumain is a predictor of all-cause mortality and potential therapeutic target in acute myocardial infarction. [Abstract]2020 Nov 26;11(11):1014. PMID: 33243972 -
Mol Cell Proteomics
Unveiling The Peptidase Network Orchestrating Hemoglobin Catabolism in Rhodnius prolixus. [Abstract]2024 Jun;23(6):100775. PMID: 38663568 -
Clin Genet
Loss-of-function Mutations in CST6 Cause Dry Skin, Desquamation and Abnormal Keratosis without Hypotrichosis. [Abstract]2023 Mar;103(3):301-309. PMID: 36371786
Solvent & Solubility
DMSO : 50 mg/mL (88.41 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.42 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (4.42 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (272 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Stern L, Perry R, Ofek P, Many A, Shabat D, Satchi-Fainaro R. A novel antitumor prodrug platform designed to be cleaved by the endoprotease legumain. Bioconjug Chem. 2009;20(3):500-510. [Content Brief]
[2]. James C. Powers, et al. Propenoyl hydrazides. US7482379B2.
[3]. Poreba M, et al. Counter Selection Substrate Library Strategy for Developing Specific Protease Substrates and Probes. Cell Chem Biol. 2016;23(8):1023-1035. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.7681 mL | 8.8406 mL | 17.6813 mL | 44.2032 mL |
| 5 mM | 0.3536 mL | 1.7681 mL | 3.5363 mL | 8.8406 mL | |
| 10 mM | 0.1768 mL | 0.8841 mL | 1.7681 mL | 4.4203 mL | |
| 15 mM | 0.1179 mL | 0.5894 mL | 1.1788 mL | 2.9469 mL | |
| 20 mM | 0.0884 mL | 0.4420 mL | 0.8841 mL | 2.2102 mL | |
| 25 mM | 0.0707 mL | 0.3536 mL | 0.7073 mL | 1.7681 mL | |
| 30 mM | 0.0589 mL | 0.2947 mL | 0.5894 mL | 1.4734 mL | |
| 40 mM | 0.0442 mL | 0.2210 mL | 0.4420 mL | 1.1051 mL | |
| 50 mM | 0.0354 mL | 0.1768 mL | 0.3536 mL | 0.8841 mL | |
| 60 mM | 0.0295 mL | 0.1473 mL | 0.2947 mL | 0.7367 mL | |
| 80 mM | 0.0221 mL | 0.1105 mL | 0.2210 mL | 0.5525 mL |
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