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  4. MeOSuc-AAPV-AMC

MeOSuc-AAPV-AMC is a fluorogenic substrate for human leukocyte and porcine pancreatic elastase (Km: 362 μM, Ex=380 nm, Em=460 nm).

For research use only. We do not sell to patients.

Custom Peptide Synthesis

MeOSuc-AAPV-AMC Chemical Structure

MeOSuc-AAPV-AMC Chemical Structure

CAS No. : 72252-90-5

Size Price Stock Quantity
1 mg USD 30 In-stock
5 mg USD 84 In-stock
10 mg USD 120 In-stock
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100 mg   Get quote  

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Based on 1 publication(s) in Google Scholar

MeOSuc-AAPV-AMC Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

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Description

MeOSuc-AAPV-AMC is a fluorogenic substrate for human leukocyte and porcine pancreatic elastase (Km: 362 μM, Ex=380 nm, Em=460 nm)[1].

IC50 & Target

Elastase[1]
In Vitro

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[1].

1. Prepare The stock solutions of fluorogenic peptide substrates and stored at -40°C.
2. Dilute the stock solution～100-fold into reaction buffers to prepare 2× substrate buffers (400 μM).
3. The enzymes are then incubated for 40 min.
4. The enzymatic activity is subsequently assayed by rapidly mixing 50 μL of the substrate buffer with 50 μL of the enzyme solution in a 96-well clear bottom plates.
5. Record the fluorescence change on a FLEXStation fluorescence plate reader.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

MeOSuc-AAPV-AMC Related Antibodies
Molecular Weight

627.69

Formula

C31H41N5O9
CAS No.
Appearance

Solid

Color

White to off-white

Emission (Em)

460
Excitation (Ex)

380
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Sealed storage, away from moisture and light

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (199.14 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.5931 mL 7.9657 mL 15.9314 mL
5 mM 0.3186 mL 1.5931 mL 3.1863 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.5931 mL 7.9657 mL 15.9314 mL 39.8286 mL
5 mM 0.3186 mL 1.5931 mL 3.1863 mL 7.9657 mL
10 mM 0.1593 mL 0.7966 mL 1.5931 mL 3.9829 mL
15 mM 0.1062 mL 0.5310 mL 1.0621 mL 2.6552 mL
20 mM 0.0797 mL 0.3983 mL 0.7966 mL 1.9914 mL
25 mM 0.0637 mL 0.3186 mL 0.6373 mL 1.5931 mL
30 mM 0.0531 mL 0.2655 mL 0.5310 mL 1.3276 mL
40 mM 0.0398 mL 0.1991 mL 0.3983 mL 0.9957 mL
50 mM 0.0319 mL 0.1593 mL 0.3186 mL 0.7966 mL
60 mM 0.0266 mL 0.1328 mL 0.2655 mL 0.6638 mL
80 mM 0.0199 mL 0.0996 mL 0.1991 mL 0.4979 mL
100 mM 0.0159 mL 0.0797 mL 0.1593 mL 0.3983 mL
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MeOSuc-AAPV-AMC Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

MeOSuc-AAPV-AMC72252-90-5Fluorescent DyeLeukocyteporcinepancreatic elastasefluorogenicsubstrateInhibitorinhibitorinhibit

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