MeOSuc-AAPV-AMC
Based on 1 Customer Validation
MeOSuc-AAPV-AMC is a fluorogenic substrate for human leukocyte and porcine pancreatic elastase (Km: 362 μM, Ex=380 nm, Em=460 nm).
For research use only. We do not sell to patients.
- Purity: 98.43%
- CAS No.: 72252-90-5
- Formula: C31H41N5O9
- Molecular Weight:627.69
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Storage:
Sealed storage, away from moisture and light.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Elastase[1]
Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[1].
1. Prepare The stock solutions of fluorogenic peptide substrates and stored at -80°C.
2. Dilute the stock solution～100-fold into reaction buffers to prepare 2× substrate buffers (400 μM).
3. The enzymes are then incubated for 40 min.
4. The enzymatic activity is subsequently assayed by rapidly mixing 50 μL of the substrate buffer with 50 μL of the enzyme solution in a clear bottom plates.
5. Record the fluorescence change on a fluorescence plate reader.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
460
380
Chemical Information
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CAS No. 72252-90-5
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Appearance Solid
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Molecular Weight 627.69
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Formula C31H41N5O9
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Color White to off-white
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture and light
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvent & Solubility
DMSO : 100 mg/mL (159.31 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (272 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.5931 mL | 7.9657 mL | 15.9314 mL | 39.8286 mL |
| 5 mM | 0.3186 mL | 1.5931 mL | 3.1863 mL | 7.9657 mL | |
| 10 mM | 0.1593 mL | 0.7966 mL | 1.5931 mL | 3.9829 mL | |
| 15 mM | 0.1062 mL | 0.5310 mL | 1.0621 mL | 2.6552 mL | |
| 20 mM | 0.0797 mL | 0.3983 mL | 0.7966 mL | 1.9914 mL | |
| 25 mM | 0.0637 mL | 0.3186 mL | 0.6373 mL | 1.5931 mL | |
| 30 mM | 0.0531 mL | 0.2655 mL | 0.5310 mL | 1.3276 mL | |
| 40 mM | 0.0398 mL | 0.1991 mL | 0.3983 mL | 0.9957 mL | |
| 50 mM | 0.0319 mL | 0.1593 mL | 0.3186 mL | 0.7966 mL | |
| 60 mM | 0.0266 mL | 0.1328 mL | 0.2655 mL | 0.6638 mL | |
| 80 mM | 0.0199 mL | 0.0996 mL | 0.1991 mL | 0.4979 mL | |
| 100 mM | 0.0159 mL | 0.0797 mL | 0.1593 mL | 0.3983 mL |
MeOSuc-AAPV-AMC Related Classifications
HY-136865 Related Classifications
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