Ac-DEVD-AFC
Based on 13 publication(s) in Google Scholar
Ac-DEVD-AFC is a fluorogenic substrate (λex=400 nm, λem=530 nm).
For research use only. We do not sell to patients.
- Purity: 98.89%
- CAS No.: 201608-14-2
- Formula: C30H34F3N5O13
- Molecular Weight:729.61
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Storage:
Sealed storage, away from moisture and light.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocol
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Ac-DEVD-AFC
More- Autophagy. 2026 May;22(5):1021-1043. [Abstract]
- JACS Au. 2024 Sep 20;4(10):3988-3999. [Abstract]
- Neoplasia. 2026 Mar 12:74:101295. [Abstract]
- Cell Rep. 2023 Apr 18;42(5):112414. [Abstract]
- J Med Chem. 2024 Nov 28;67(22):20372-20398. [Abstract]
- J Ethnopharmacol. 2020 Nov 15;262:113213. [Abstract]
- Mar Life Sci Technol. 2024 Jun 28;6(3):462-474. [Abstract]
- J Mol Struct. 2024 April 5.
- Fish Shellfish Immunol. 2026 Jun:173:111286. [Abstract]
- Aquaculture. 2025 Oct 15.
- Fish Shellfish Immunol. 2024 Nov 2:110002. [Abstract]
- ACS Pharmacol Transl Sci. 2021 Jun 9;4(4):1408-1421. [Abstract]
- Genes (Basel). 2024 Apr 25;15(5):540. [Abstract]
Biological Activity
After incubation with Ac-DEVD-AFC for 1 hour, significant increase of caspase-3 activity is observed at 4 hour compare with control. There are no significant increases of caspase-3 activity in Photofrin and LPLI group. The cleavage of Ac-DEVD-AFC in response to caspase-3 activation is remarkably inhibited by shRNA-BimL transfection[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Chemical Information
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CAS No. 201608-14-2
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Appearance Solid
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Molecular Weight 729.61
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Formula C30H34F3N5O13
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Color White to yellow
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Sequence
N-Acetyl-Asp-Glu-Val-Asp-7-amido-4-trifluoroMethylcoumarin
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Sequence Shortening
Ac-DEVD-7-amido-4-trifluoroMethylcoumarin
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture and light
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (13)
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Journal Impact Factor
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Most Recent
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Autophagy
Phase separation of OPTN initiates mitophagy to orchestrate craniofacial bone mineralization. [Abstract]2026 May;22(5):1021-1043. PMID: 41692957 -
JACS Au
2024 Sep 20;4(10):3988-3999. PMID: 39483232 -
Neoplasia
Rational payload selection enables high antitumoral efficacy of an anti-EGFR antibody-drug conjugate against ovarian tumors. [Abstract]2026 Mar 12:74:101295. PMID: 41825107 -
Cell Rep
2023 Apr 18;42(5):112414. PMID: 37074912 -
J Med Chem
Discovery of Thiazole Carboxamides as Novel Vanin-1 Inhibitors for Inflammatory Bowel Disease Treatment. [Abstract]2024 Nov 28;67(22):20372-20398. PMID: 39514323 -
J Ethnopharmacol
Ganoderic acid D induces synergistic autophagic cell death except for apoptosis in ESCC cells. [Abstract]2020 Nov 15;262:113213. PMID: 32755651 -
Mar Life Sci Technol
Pufferfish gasdermin Ea is a significant player in the defense against bacterial pathogens. [Abstract]2024 Jun 28;6(3):462-474. PMID: 39219679 -
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Fish Shellfish Immunol
Spotted seabass Lateolabrax maculatus GSDMEa: a pyroptosis executor activated by CASP3/7 during bacterial infection. [Abstract]2026 Jun:173:111286. PMID: 41864287 -
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Fish Shellfish Immunol
Paralichthys olivaceus GSDME-mediated pyroptosis is regulated by multiple caspases in different manners. [Abstract]2024 Nov 2:110002. PMID: 39491658 -
ACS Pharmacol Transl Sci
Rational Design of Hybrid SARS-CoV-2 Main Protease Inhibitors Guided by the Superimposed Cocrystal Structures with the Peptidomimetic Inhibitors GC-376, Telaprevir, and Boceprevir. [Abstract]2021 Jun 9;4(4):1408-1421. PMID: 34414360 -
Genes (Basel)
Characterization of the Apoptotic and Antimicrobial Activities of Two Initiator Caspases of Sea Cucumber Apostichopus japonicus. [Abstract]2024 Apr 25;15(5):540. PMID: 38790170
Solvent & Solubility
DMSO : ≥ 50 mg/mL (68.53 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : < 0.1 mg/mL (insoluble)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocol
For the detection of caspase-3 activity, PBS washes cell pellets (derive from either the medium or the adherent cells) which are suspended in extract buffer [25 mM HEPES (pH7.4), 0.1% TritonX-l00, 10% glycerol, 5 mM DTT, 1mM phenylmethylsulfonyl fluoride, 10 mg/mL pepstatin, and 10 mg/mL Leupeptin] and vortexed vigorously. 20μl of extract (corresponding to 10% of the sample) are incubated with the caspase-3 fluorogenic substrates Ac-DEVD-AFC at 100 μM final concentration at room temperature, and caspase-3 activity is measured continuously by monitoring the release of fluorigenic AFC at 37°C[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (278 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.3706 mL | 6.8530 mL | 13.7060 mL | 34.2649 mL |
| 5 mM | 0.2741 mL | 1.3706 mL | 2.7412 mL | 6.8530 mL | |
| 10 mM | 0.1371 mL | 0.6853 mL | 1.3706 mL | 3.4265 mL | |
| 15 mM | 0.0914 mL | 0.4569 mL | 0.9137 mL | 2.2843 mL | |
| 20 mM | 0.0685 mL | 0.3426 mL | 0.6853 mL | 1.7132 mL | |
| 25 mM | 0.0548 mL | 0.2741 mL | 0.5482 mL | 1.3706 mL | |
| 30 mM | 0.0457 mL | 0.2284 mL | 0.4569 mL | 1.1422 mL | |
| 40 mM | 0.0343 mL | 0.1713 mL | 0.3426 mL | 0.8566 mL | |
| 50 mM | 0.0274 mL | 0.1371 mL | 0.2741 mL | 0.6853 mL | |
| 60 mM | 0.0228 mL | 0.1142 mL | 0.2284 mL | 0.5711 mL |
Ac-DEVD-AFC Related Classifications
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