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  4. Ac-DEVD-AFC

Ac-DEVD-AFC 

Cat. No.: HY-P1005 Purity: 99.24%
COA Handling Instructions

Ac-DEVD-AFC is a fluorogenic substrate (λex=400 nm, λem=530 nm). Storage: protect from light.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Ac-DEVD-AFC Chemical Structure

Ac-DEVD-AFC Chemical Structure

CAS No. : 201608-14-2

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1 mg USD 60 In-stock
5 mg USD 180 In-stock
10 mg USD 336 In-stock
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Customer Review

Based on 3 publication(s) in Google Scholar

Top Publications Citing Use of Products

  • Biological Activity

  • Protocol

  • Purity & Documentation

  • References

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Description

Ac-DEVD-AFC is a fluorogenic substrate (λex=400 nm, λem=530 nm). Storage: protect from light.

In Vitro

After incubation with Ac-DEVD-AFC for 1 hour, significant increase of caspase-3 activity is observed at 4 hour compare with control. There are no significant increases of caspase-3 activity in Photofrin and LPLI group. The cleavage of Ac-DEVD-AFC in response to caspase-3 activation is remarkably inhibited by shRNA-BimL transfection[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

729.61

Appearance

Solid

Formula

C30H34F3N5O13
CAS No.
Emission (Em)

500
Excitation (Ex)

380
Sequence

N-Acetyl-Asp-Glu-Val-Asp-7-amido-4-trifluoroMethylcoumarin
Sequence Shortening

Ac-DEVD-7-amido-4-trifluoroMethylcoumarin
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Sealed storage, away from moisture and light

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvent & Solubility
In Vitro: 

DMSO : ≥ 50 mg/mL (68.53 mM)

H2O : 0.67 mg/mL (0.92 mM; Need ultrasonic)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.3706 mL 6.8530 mL 13.7060 mL
5 mM 0.2741 mL 1.3706 mL 2.7412 mL
10 mM 0.1371 mL 0.6853 mL 1.3706 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (3.43 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (3.43 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (3.43 mM); Clear solution

*All of the co-solvents are available by MCE.
Purity & Documentation
References
Cell Assay
[1]

For the detection of caspase-3 activity, PBS washes cell pellets (derive from either the medium or the adherent cells) which are suspended in extract buffer [25 mM HEPES (pH7.4), 0.1% TritonX-l00, 10% glycerol, 5 mM DTT, 1mM phenylmethylsulfonyl fluoride, 10 mg/mL pepstatin, and 10 mg/mL Leupeptin] and vortexed vigorously. 20μl of extract (corresponding to 10% of the sample) are incubated with the caspase-3 fluorogenic substrates Ac-DEVD-AFC at 100 μM final concentration at room temperature, and caspase-3 activity is measured continuously by monitoring the release of fluorigenic AFC at 37°C[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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This equation is commonly abbreviated as: C1V1 = C2V2

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Keywords:

Ac-DEVD-AFC201608-14-2Fluorescent DyeInhibitorinhibitorinhibit

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