Abz-Val-Ala-Asp-Nva-Arg-Asp-Arg-Gln-EDDnp
Based on 1 Customer Validation
Abz-Val-Ala-Asp-Nva-Arg-Asp-Arg-Gln-EDDnp is a fluorescent FRET substrate for proteinase 3 (PR3) with selectivity over neutrophil elastase and cathepsin G. Abz-Val-Ala-Asp-Nva-Arg-Asp-Arg-Gln-EDDnp is cleaved by active PR3 to generate a detectable fluorescent signal, which is used to quantify the enzymatic activity of PR3, the concentration of free or membrane-bound PR3, and the PR3 activity on activated human neutrophils. Abz-Val-Ala-Asp-Nva-Arg-Asp-Arg-Gln-EDDnp can be applied in studies related to lung ischemia-reperfusion injury, inflammatory diseases, and other relevant conditions.
For research use only. We do not sell to patients.
- Purity : 99.49%
- CAS No.: 1042405-14-0
- Formula: C53H80N20O18
- Molecular Weight:1285.33
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Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Description
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PR3 |
In Vitro
Abz-Val-Ala-Asp-Nva-Arg-Asp-Arg-Gln-EDDnp (1 mM; 30 min) serves as a fluorescent substrate for detecting PR3 activity in human polymorphonuclear neutrophils via real-time fluorescence measurement[1].
Abz-Val-Ala-Asp-Nva-Arg-Asp-Arg-Gln-EDDnp (20 μM; 2×106-2×106 cells per well) enables selective measurement of membrane-bound proteinase 3 activity on activated human neutrophils[2].
Abz-Val-Ala-Asp-Nva-Arg-Asp-Arg-Gln-EDDnp is a highly selective fluorogenic substrate for human proteinase 3, with a kcat/Km of 1,571 mM-1s-1, and shows minimal reactivity with human neutrophil elastase and human cathepsin G[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 1042405-14-0
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Appearance Solid
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Molecular Weight 1285.33
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Formula C53H80N20O18
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Color White to yellow
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Sequence
{Abz}-Val-Ala-Asp-{Nva}-Arg-Asp-Arg-Gln-{EDDnp}
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Sequence Shortening
{Abz}-VAD-{Nva}-RDDQ-{EDDnp}
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (77.80 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (282 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Yu J, et al. Cathepsin C from extracellular histone-induced M1 alveolar macrophages promotes NETosis during lung ischemia-reperfusion injury. Redox biology. 2024 Aug;74:103231. [Content Brief]
[2]. Korkmaz B, et al. Measurement of neutrophil elastase, proteinase 3, and cathepsin G activities using intramolecularly quenched fluorogenic substrates. Methods in molecular biology (Clifton, N.J.). 2012;844:125-38. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 0.7780 mL | 3.8901 mL | 7.7801 mL | 19.4503 mL |
| 5 mM | 0.1556 mL | 0.7780 mL | 1.5560 mL | 3.8901 mL | |
| 10 mM | 0.0778 mL | 0.3890 mL | 0.7780 mL | 1.9450 mL | |
| 15 mM | 0.0519 mL | 0.2593 mL | 0.5187 mL | 1.2967 mL | |
| 20 mM | 0.0389 mL | 0.1945 mL | 0.3890 mL | 0.9725 mL | |
| 25 mM | 0.0311 mL | 0.1556 mL | 0.3112 mL | 0.7780 mL | |
| 30 mM | 0.0259 mL | 0.1297 mL | 0.2593 mL | 0.6483 mL | |
| 40 mM | 0.0195 mL | 0.0973 mL | 0.1945 mL | 0.4863 mL | |
| 50 mM | 0.0156 mL | 0.0778 mL | 0.1556 mL | 0.3890 mL | |
| 60 mM | 0.0130 mL | 0.0648 mL | 0.1297 mL | 0.3242 mL |
Abz-Val-Ala-Asp-Nva-Arg-Asp-Arg-Gln-EDDnp Related Classifications
HY-P10461 Related Classifications
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Keywords
- Abz-Val-Ala-Asp-Nva-Arg-Asp-Arg-Gln-EDDnp
- 1042405-14-0
- Amino Acid Derivatives
- Elastase
- Cathepsin
- cyclic neutropenia
- Proteinase 3
- Wegener’s granulomatosis
- neutrophil elastase
- chronic lung diseases
- cathepsin G
- severe congenital neutropenia
- human neutrophils
- inflammatory diseases
- lung ischemia-reperfusion injury
- Inhibitor
- inhibitor
- inhibit