Z-Leu-Leu-Glu-AMC
Based on 3 publication(s) in Google Scholar
Z-Leu-Leu-Glu-AMC (Z-LLE-AMC) is a peptide-AMC linked substrate used to measure the postacidic-like hydrolysing activity of proteasome. Z-Leu-Leu-Glu-AMC can be used for the research of parkinson's disease.
For research use only. We do not sell to patients.
- Purity: 99.73%
- CAS No.: 348086-66-8
- Formula: C35H44N4O9
- Molecular Weight:664.75
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Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Z-Leu-Leu-Glu-AMC
More
Biological Activity
Z-Leu-Leu-Glu-AMC (75 μM) is used as a substrate to detect proteasomal postacidic-like activity, which is reduced by 33% in substantia nigra pars compacta homogenates of idiopathic Parkinson’s disease[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 348086-66-8
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Appearance Solid
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Molecular Weight 664.75
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Formula C35H44N4O9
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Color White to off-white
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Synonyms
Z-LLE-AMC
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Sequence
{Z}-Leu-Leu-Glu-{AMC}
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Sequence Shortening
{Z}-LLE-{AMC}
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (3)
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Journal Impact Factor
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Most Recent
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Protein Sci
Distinct substrate specificities of the three catalytic subunits of the Trichomonas vaginalis proteasome. [Abstract]2024 Dec;33(12):e5225. PMID: 39589076 -
Neurochem Int
Proteostasis and autophagy disruption by the aging-related VGVAPG hexapeptide - preliminary insights into a potential novel elastin-induced neurodegeneration pathway in an in vitro human cellular neuron model. [Abstract]2025 Jul:187:105992. PMID: 40348194 -
Solvent & Solubility
DMSO : 100 mg/mL (150.43 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (273 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.5043 mL | 7.5216 mL | 15.0432 mL | 37.6081 mL |
| 5 mM | 0.3009 mL | 1.5043 mL | 3.0086 mL | 7.5216 mL | |
| 10 mM | 0.1504 mL | 0.7522 mL | 1.5043 mL | 3.7608 mL | |
| 15 mM | 0.1003 mL | 0.5014 mL | 1.0029 mL | 2.5072 mL | |
| 20 mM | 0.0752 mL | 0.3761 mL | 0.7522 mL | 1.8804 mL | |
| 25 mM | 0.0602 mL | 0.3009 mL | 0.6017 mL | 1.5043 mL | |
| 30 mM | 0.0501 mL | 0.2507 mL | 0.5014 mL | 1.2536 mL | |
| 40 mM | 0.0376 mL | 0.1880 mL | 0.3761 mL | 0.9402 mL | |
| 50 mM | 0.0301 mL | 0.1504 mL | 0.3009 mL | 0.7522 mL | |
| 60 mM | 0.0251 mL | 0.1254 mL | 0.2507 mL | 0.6268 mL | |
| 80 mM | 0.0188 mL | 0.0940 mL | 0.1880 mL | 0.4701 mL | |
| 100 mM | 0.0150 mL | 0.0752 mL | 0.1504 mL | 0.3761 mL |
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