Suc-Leu-Leu-Val-Tyr-AMC
Based on 16 publication(s) in Google Scholar
Suc-Leu-Leu-Val-Tyr-AMC is a membrane-permeable calpain-specific fluorogenic substrate (Ex/Em = 390/480 nm).
For research use only. We do not sell to patients.
- Purity: 99.96%
- CAS No.: 94367-21-2
- Formula: C40H53N5O10
- Molecular Weight:763.88
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Storage:
Sealed storage, away from moisture and light, under nitrogen.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocol
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Suc-Leu-Leu-Val-Tyr-AMC
More- Nature. 2022 May;605(7910):567-574. [Abstract]
- Nat Commun. 2025 Feb 14;16(1):1634. [Abstract]
- Environ Sci Technol. 2019 Aug 20;53(16):9789-9799. [Abstract]
- Phytomedicine. 2025 Jun:141:156647. [Abstract]
- Int J Biol Macromol. 2024 Nov;280(Pt 2):135764. [Abstract]
- Food Funct. 2021 Nov 1;12(21):10773-10782. [Abstract]
- Cell Biol Toxicol. 2024 Jul 29;40(1):60. [Abstract]
- Protein Sci. 2024 Dec;33(12):e5225. [Abstract]
- Neurochem Int. 2025 Jul:187:105992. [Abstract]
- PLoS Pathog. 2026 Feb 12;22(2):e1013909. [Abstract]
- FASEB J. 2025 Oct 31;39(20):e71135. [Abstract]
- Neuroscience. 2024 May 10:549:101-109. [Abstract]
- Vet Microbiol. 2026 Feb:313:110835. [Abstract]
- Res Sq. 2026 Mar 2.
- bioRxiv. 2024 Aug 7:2024.08.05.606617. [Abstract]
- BMC Dev Biol. 2021 Feb 1;21(1):4. [Abstract]
Customer Validation & Images
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Bio/Physico-chemical Assay
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Bio/Physico-chemical Assay
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Bio/Physico-chemical Assay
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Bio/Physico-chemical Assay
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Bio/Physico-chemical Assay
Biological Activity
Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
1. Solution preparation[1]
1.1 Preparation of stock solution
Solvent: DMSO
Concentration: 62.5 mM is recommended.
Storage: Store at -20°C or -80°C in dark after aliquoting. Avoid repeated freezing and thawing.
1.2 Preparation of working solution
Dilute to 6.25 μM with assay buffer (optimized according to the experiment).
Note: The working solution should be prepared and used immediately. Keep it away from light.
2. Cell staining
2.1 L929 cells are plated at 105 cells in 200 μL per well in 96-well plates.
2.2 Add 200 μL of assay buffer (115 mM NaCl, 1 mM KH2PO4, 5 mM KCl, 2 mM CaCl2, 1.2 mM MgSO4, 25 mM sodium HEPES buffer (pH 7.4)) to all wells.
2.3 Add Suc-Leu-Leu-Val-Tyr-AMC (62.5 μM) to the cells.
2.4 The plates are incubated at 37°C in the fluorometer, and fluorescence was measured at 5-min intervals starting immediately after the addition of substrate.
Suc-Leu-Leu-Val-Tyr-AMC (Suc-LLVY) is a membrane-permeable calpain-specific fluorogenic substrate, pteolytic hydrolysis of the peptidyl-7-amino bond liberates the highly fluorescent 7-amino-4-methylcoumarin (AMC) moiety[1]. The effect
of TGF-β on hydrolysis of these substrates (e.g Suc-Leu-Leu-Val-Tyr-AMC) are assessed. Biliary epithelial H69 cells are incubated with 10, 1, 0.1, or 0 ng/mL TGF-β for 24 h. Substrate hydrolysis is then fluorometrically assessed in cytosolic extracts. Basal activity is 1.12, 8.33, and 14.52 nmol AMC/mg protein/min for suc-LLVY-AMC, z-LLE-AMC, and z-LLL-AMC hydrolysis, respectively[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Chemical Information
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CAS No. 94367-21-2
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Appearance Solid
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Molecular Weight 763.88
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Formula C40H53N5O10
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Color White to off-white
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Synonyms
Suc-LLVY-AMC
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Sequence
Suc-Leu-Leu-Val-Tyr-AMC
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Sequence Shortening
Suc-LLVY-AMC
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture and light, under nitrogen
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)
Publications (16)
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Journal Impact Factor
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Most Recent
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Nature
2022 May;605(7910):567-574. PMID: 35477760 -
Nat Commun
Proteasomes accumulate in the plant apoplast where they participate in microbe-associated molecular pattern (MAMP)-triggered pathogen defense. [Abstract]2025 Feb 14;16(1):1634. PMID: 39952938
Suc-Leu-Leu-Val-Tyr-AMC purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Feb 14;16(1):1634. [Abstract]
Suc-LLVY-AMC (100 µM; 20 min). Specific activity per mg protein for proteasomes in the APFp and CL based on hydrolysis of the CP substrate Suc-LLVY-AMC.
Suc-Leu-Leu-Val-Tyr-AMC purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Feb 14;16(1):1634. [Abstract]
Suc-LLVY-AMC (100 µM; 20 min). Sensitivity of proteasomes in the CL and APF from Arabidopsis leaves to a collection of inhibitors designed to block either proteasomes or various classes of peptidases/proteases, a 1X concentration of protease-inhibitor cocktail (PIC) that inhibits a collection of peptidases/proteases, or the activity of the CDC48 chaperone. The CL and APF were pretreated with the indicated concentrations for 5 min before assay.
Suc-Leu-Leu-Val-Tyr-AMC purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Feb 14;16(1):1634. [Abstract]
Suc-LLVY-AMC (100 µM; 20 min). Sensitivity of Suc-LLVY-AMC cleavage by the APF to a combination of 1X PIC and 50 µM BTZ.
Suc-Leu-Leu-Val-Tyr-AMC purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Feb 14;16(1):1634. [Abstract]
Suc-LLVY-AMC (100 µM; 20 min). Arabidopsis ex-proteasome activity is inhibited by SylA. The crude lysate (CL), APF, and affinity-purified proteasomes were extracted from PAG1-FLAG pag1-1 leaves, pretreated for 20 min without or with 50 or 150 µM of SylA or an equivalent volume of DMSO (Mock), and assayed for proteasomes activity using the fluorogenic substrate Suc-LLVY-AMC.
Suc-Leu-Leu-Val-Tyr-AMC purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Feb 14;16(1):1634. [Abstract]
Suc-LLVY-AMC (100 µM; 20 min). Proteolytic activity of proteasomes in the APFp samples using the fluorescent Suc-LLVY-AMC substrate with or without 50 µM BTZ.
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Environ Sci Technol
Particulate Matter Exposure History Affects Antioxidant Defense Response of Mouse Lung to Haze Episodes. [Abstract]2019 Aug 20;53(16):9789-9799. PMID: 31328514 -
Phytomedicine
Xerophenone H, a naturally-derived proteasome inhibitor, triggers apoptosis and paraptosis in lung cancer. [Abstract]2025 Jun:141:156647. PMID: 40112632 -
Int J Biol Macromol
2024 Nov;280(Pt 2):135764. PMID: 39299429 -
Food Funct
A dipeptidyl peptidase IV inhibitory peptide relieves palmitic acid-induced endoplasmic reticulum stress in HepG2 cells independent of inhibiting dipeptidyl peptidase IV activity. [Abstract]2021 Nov 1;12(21):10773-10782. PMID: 34609396 -
Cell Biol Toxicol
Proteasome activity inhibition mediates endoplasmic reticulum stress-apoptosis in triptolide/lipopolysaccharide-induced hepatotoxicity. [Abstract]2024 Jul 29;40(1):60. PMID: 39073694 -
Protein Sci
Distinct substrate specificities of the three catalytic subunits of the Trichomonas vaginalis proteasome. [Abstract]2024 Dec;33(12):e5225. PMID: 39589076 -
Neurochem Int
Proteostasis and autophagy disruption by the aging-related VGVAPG hexapeptide - preliminary insights into a potential novel elastin-induced neurodegeneration pathway in an in vitro human cellular neuron model. [Abstract]2025 Jul:187:105992. PMID: 40348194 -
PLoS Pathog
Structural and mechanistic insights into caseinolytic protease inhibition for antimicrobial development against Pseudomonas plecoglossicida. [Abstract]2026 Feb 12;22(2):e1013909. PMID: 41678539 -
FASEB J
Protein Arginine N-Methyltransferase 4 Activation Contributes to Glucose-Induced Skeletal Muscle Atrophy. [Abstract]2025 Oct 31;39(20):e71135. PMID: 41078314 -
Neuroscience
2024 May 10:549:101-109. PMID: 38734303 -
Vet Microbiol
PRRSV Nsp12 targets PSMA2 to suppress host proteasome activity and promote viral survival. [Abstract]2026 Feb:313:110835. PMID: 41475191 -
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bioRxiv
Mast cell-derived chymases are essential for the resolution of inflammatory pain in mice. [Abstract]2024 Aug 7:2024.08.05.606617. PMID: 39211156 -
BMC Dev Biol
Generation and identification of a conditional knockout allele for the PSMD11 gene in mice. [Abstract]2021 Feb 1;21(1):4. PMID: 33517884
Solvent & Solubility
DMSO : 50 mg/mL (65.46 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocol
Immunoprecipitation is carried out for the two sets of samples, using the same amount of protein. The 20 S and 26 S proteasome immunoprecipitates are washed with 50 mM Hepes/KOH (pH 7.5), and 50 mM Hepes/KOH (pH 7.5) containing 2 mMATP, respectively, prior to the determination of peptidase activity using 50 μM suc-Leu- Leu-Val-Tyr-AMC as substrate in these buffers[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (287 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
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Handling Instructions (2659 KB)
References
[1]. Roberta L. Debiasi, et al. Reovirus-Induced Apoptosis Is Preceded by Increased Cellular Calpain Activity and Is Blocked by Calpain Inhibitors. J Virol. 1999 Jan; 73(1): 695-701. [Content Brief]
[2]. Tadlock L, et al. Transforming growth factor-beta inhibition of proteasomal activity: a potential mechanism of growth arrest. Am J Physiol Cell Physiol. 2003 Aug;285(2):C277-85. Epub 2003 Mar 19. [Content Brief]
[3]. Gardner RC, et al. Characterization of peptidyl boronic acid inhibitors of mammalian 20 S and 26 S proteasomes and their inhibition of proteasomes in cultured cells. Biochem J. 2000 Mar, 2:447-54. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.3091 mL | 6.5455 mL | 13.0911 mL | 32.7277 mL |
| 5 mM | 0.2618 mL | 1.3091 mL | 2.6182 mL | 6.5455 mL | |
| 10 mM | 0.1309 mL | 0.6546 mL | 1.3091 mL | 3.2728 mL | |
| 15 mM | 0.0873 mL | 0.4364 mL | 0.8727 mL | 2.1818 mL | |
| 20 mM | 0.0655 mL | 0.3273 mL | 0.6546 mL | 1.6364 mL | |
| 25 mM | 0.0524 mL | 0.2618 mL | 0.5236 mL | 1.3091 mL | |
| 30 mM | 0.0436 mL | 0.2182 mL | 0.4364 mL | 1.0909 mL | |
| 40 mM | 0.0327 mL | 0.1636 mL | 0.3273 mL | 0.8182 mL | |
| 50 mM | 0.0262 mL | 0.1309 mL | 0.2618 mL | 0.6546 mL | |
| 60 mM | 0.0218 mL | 0.1091 mL | 0.2182 mL | 0.5455 mL |
Suc-Leu-Leu-Val-Tyr-AMC Related Classifications
HY-P1002 Related Classifications
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