2. Others Metabolic Enzyme/Protease
  3. Fluorescent Dye Proteasome
  4. Suc-Leu-Leu-Val-Tyr-AMC

Suc-Leu-Leu-Val-Tyr-AMC  (Synonyms: Suc-LLVY-AMC)

Cat. No.: HY-P1002 Purity: 99.66%
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Suc-Leu-Leu-Val-Tyr-AMC is a membrane-permeable calpain-specific fluorogenic substrate (Ex/Em = 390/480 nm).

For research use only. We do not sell to patients.

Custom Peptide Synthesis

CAS No. : 94367-21-2

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Customer Review

Based on 16 publication(s) in Google Scholar

Other Forms of Suc-Leu-Leu-Val-Tyr-AMC:

Suc-Leu-Leu-Val-Tyr-AMC Related Antibodies

Top Publications Citing Use of Products

    Suc-Leu-Leu-Val-Tyr-AMC purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Feb 14;16(1):1634.  [Abstract]

    Suc-LLVY-AMC (100 µM; 20 min). Specific activity per mg protein for proteasomes in the APFp and CL based on hydrolysis of the CP substrate Suc-LLVY-AMC.

    Suc-Leu-Leu-Val-Tyr-AMC purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Feb 14;16(1):1634.  [Abstract]

    Suc-LLVY-AMC (100 µM; 20 min). Sensitivity of proteasomes in the CL and APF from Arabidopsis leaves to a collection of inhibitors designed to block either proteasomes or various classes of peptidases/proteases, a 1X concentration of protease-inhibitor cocktail (PIC) that inhibits a collection of peptidases/proteases, or the activity of the CDC48 chaperone. The CL and APF were pretreated with the indicated concentrations for 5 min before assay.

    Suc-Leu-Leu-Val-Tyr-AMC purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Feb 14;16(1):1634.  [Abstract]

    Suc-LLVY-AMC (100 µM; 20 min). Sensitivity of Suc-LLVY-AMC cleavage by the APF to a combination of 1X PIC and 50 µM BTZ.

    Suc-Leu-Leu-Val-Tyr-AMC purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Feb 14;16(1):1634.  [Abstract]

    Suc-LLVY-AMC (100 µM; 20 min). Arabidopsis ex-proteasome activity is inhibited by SylA. The crude lysate (CL), APF, and affinity-purified proteasomes were extracted from PAG1-FLAG pag1-1 leaves, pretreated for 20 min without or with 50 or 150 µM of SylA or an equivalent volume of DMSO (Mock), and assayed for proteasomes activity using the fluorogenic substrate Suc-LLVY-AMC.

    Suc-Leu-Leu-Val-Tyr-AMC purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Feb 14;16(1):1634.  [Abstract]

    Suc-LLVY-AMC (100 µM; 20 min). Proteolytic activity of proteasomes in the APFp samples using the fluorescent Suc-LLVY-AMC substrate with or without 50 µM BTZ.

    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Suc-Leu-Leu-Val-Tyr-AMC is a membrane-permeable calpain-specific fluorogenic substrate (Ex/Em = 390/480 nm)[1].

    In Vitro

    Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
    1. Solution preparation[1]
    1.1 Preparation of stock solution
    Solvent: DMSO
    Concentration: 62.5 mM is recommended.
    Storage: Store at -20°C or -80°C in dark after aliquoting. Avoid repeated freezing and thawing.
    1.2 Preparation of working solution
    Dilute to 6.25 μM with assay buffer (optimized according to the experiment).
    Note: The working solution should be prepared and used immediately. Keep it away from light.

    2. Cell staining
    2.1 L929 cells are plated at 105 cells in 200 μL per well in 96-well plates.
    2.2 Add 200 μL of assay buffer (115 mM NaCl, 1 mM KH2PO4, 5 mM KCl, 2 mM CaCl2, 1.2 mM MgSO4, 25 mM sodium HEPES buffer (pH 7.4)) to all wells.
    2.3 Add Suc-Leu-Leu-Val-Tyr-AMC (62.5 μM) to the cells.
    2.4 The plates are incubated at 37°C in the fluorometer, and fluorescence was measured at 5-min intervals starting immediately after the addition of substrate.
    Suc-Leu-Leu-Val-Tyr-AMC (Suc-LLVY) is a membrane-permeable calpain-specific fluorogenic substrate, pteolytic hydrolysis of the peptidyl-7-amino bond liberates the highly fluorescent 7-amino-4-methylcoumarin (AMC) moiety[1]. The effect of TGF-β on hydrolysis of these substrates (e.g Suc-Leu-Leu-Val-Tyr-AMC) are assessed. Biliary epithelial H69 cells are incubated with 10, 1, 0.1, or 0 ng/mL TGF-β for 24 h. Substrate hydrolysis is then fluorometrically assessed in cytosolic extracts. Basal activity is 1.12, 8.33, and 14.52 nmol AMC/mg protein/min for suc-LLVY-AMC, z-LLE-AMC, and z-LLL-AMC hydrolysis, respectively[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Suc-Leu-Leu-Val-Tyr-AMC Related Antibodies
    Molecular Weight

    763.88

    Formula

    C40H53N5O10
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    Emission (Em)

    480
    Excitation (Ex)

    390
    Sequence

    Suc-Leu-Leu-Val-Tyr-AMC
    Sequence Shortening

    Suc-LLVY-AMC
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    Sealed storage, away from moisture and light, under nitrogen

    Powder -80°C 2 years
    -20°C 1 year

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)

    Solvent & Solubility
    In Vitro: 

    DMSO : 50 mg/mL (65.46 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.3091 mL 6.5455 mL 13.0911 mL
    5 mM 0.2618 mL 1.3091 mL 2.6182 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
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    Volume
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

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    Volume (start)

    V1

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    Concentration (final)

    C2

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    Volume (final)

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    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    Purity & Documentation

    Purity: 99.66%

    SDS (396 KB)

    COA (260 KB) RP-HPLC (251 KB) MS (69 KB)

    Handling Instructions (2659 KB)
    References
    Kinase Assay
    [3]

    Immunoprecipitation is carried out for the two sets of samples, using the same amount of protein. The 20 S and 26 S proteasome immunoprecipitates are washed with 50 mM Hepes/KOH (pH 7.5), and 50 mM Hepes/KOH (pH 7.5) containing 2 mMATP, respectively, prior to the determination of peptidase activity using 50 μM suc-Leu- Leu-Val-Tyr-AMC as substrate in these buffers[3].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.3091 mL 6.5455 mL 13.0911 mL 32.7277 mL
    5 mM 0.2618 mL 1.3091 mL 2.6182 mL 6.5455 mL
    10 mM 0.1309 mL 0.6546 mL 1.3091 mL 3.2728 mL
    15 mM 0.0873 mL 0.4364 mL 0.8727 mL 2.1818 mL
    20 mM 0.0655 mL 0.3273 mL 0.6546 mL 1.6364 mL
    25 mM 0.0524 mL 0.2618 mL 0.5236 mL 1.3091 mL
    30 mM 0.0436 mL 0.2182 mL 0.4364 mL 1.0909 mL
    40 mM 0.0327 mL 0.1636 mL 0.3273 mL 0.8182 mL
    50 mM 0.0262 mL 0.1309 mL 0.2618 mL 0.6546 mL
    60 mM 0.0218 mL 0.1091 mL 0.2182 mL 0.5455 mL
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    Suc-Leu-Leu-Val-Tyr-AMC Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Suc-Leu-Leu-Val-Tyr-AMC94367-21-2Suc-LLVY-AMCFluorescent DyeProteasomeInhibitorinhibitorinhibit

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