Cat. No.: HY-D0843

N-Ethylmaleimide

(Synonyms: NEM)

44 Cited Publications

Customer Review

Based on 44 publication(s) in Google Scholar

N-Ethylmaleimide (NEM) derives from maleic acid, it can alkylates free sulfhydryl. N-Ethylmaleimide is an irreversible cysteine protease inhibitor. N-ethylmaleimide specific inhibits phosphate transport in mitochondria. N-Ethylmaleimide inhibits prolyl endopeptidase with an IC₅₀ value of 6.3 μM. N-Ethylmaleimide can be used to modify cysteine residues in proteins and peptides.

For research use only. We do not sell to patients.

Purity: 99.59%
CAS No.: 128-53-0
Formula: C₆H₇NO₂
Molecular Weight:125.13

Storage:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

N-Ethylmaleimide Chemical Structure

CAS No.: 128-53-0

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Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
- Clinical Trial
Chemical Information
Publications (44)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

IC50: 6.3 μM (prolyl endopeptidase)^[2]

All Cathepsin Isoforms :

All Deubiquitinase Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
COS-7	EC₅₀	43.7 μM Compound: NEM	Binding affinity to rat Galpha-fused ORL1 receptor expressed in african green monkey COS7 cells after 90 mins Binding affinity to rat Galpha-fused ORL1 receptor expressed in african green monkey COS7 cells after 90 mins	[PMID: 22061823]
KB 3-1	IC₅₀	0.03 mM Compound: N-ethyl maleimide	Cytotoxicity against human KB-3-1 cells incubated for 72 hrs by MTT assay Cytotoxicity against human KB-3-1 cells incubated for 72 hrs by MTT assay	[PMID: 21657271]
KB-V1	IC₅₀	0.016 mM Compound: N-ethyl maleimide	Cytotoxicity against drug-resistant human KBV1 cells expressing P-gp incubated for 72 hrs by MTT assay Cytotoxicity against drug-resistant human KBV1 cells expressing P-gp incubated for 72 hrs by MTT assay	[PMID: 21657271]
MCF7	GI₅₀	9.9 μg/mL Compound: 45	Cytotoxicity against human MCF7 cells assessed as cell growth after 2 days by sulforhodamine B assay Cytotoxicity against human MCF7 cells assessed as cell growth after 2 days by sulforhodamine B assay	[PMID: 21481593]
NCI-H460	GI₅₀	6.7 μg/mL Compound: 45	Cytotoxicity against human H460 cells assessed as cell growth after 2 days by sulforhodamine B assay Cytotoxicity against human H460 cells assessed as cell growth after 2 days by sulforhodamine B assay	[PMID: 21481593]
SF-268	GI₅₀	6.5 μg/mL Compound: 45	Cytotoxicity against human SF268 cells assessed as cell growth after 2 days by sulforhodamine B assay Cytotoxicity against human SF268 cells assessed as cell growth after 2 days by sulforhodamine B assay	[PMID: 21481593]

In Vitro

N-Ethylmaleimide (20 μM;30 min) inhibits Akt Ser-473, Akt Thr-308 , p70S6K, ribosomal protein S6, 4E-BP1, eIF4E, BAD and FKHR-L1 phosphorylation^[2].
N-Ethylmaleimide (20 μM;30 min) affects conversion of pro-caspase-3 in vascular smooth muscle cells^[2].
N-Ethylmaleimide (20 μM;6 h) promotes vascular smooth muscle cells apoptosis^[2].
N-Ethylmaleimide (20 μM;30 min) affects PP2A activity and ROS production in vascular smooth muscle cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[2]

Cell Line:

Vascular smooth muscle cells
Concentration:

20 μM
Incubation Time:

2 hours
Result:

Effectively inhibited platelet-derived growth factor-BB (PDGF-BB)-stimulated Akt Ser-473 , Akt Thr-308, p70S6K, ribosomal protein S6, 4E-BP1, BAD and FKHR-L1 phosphorylation with a concentration of 20 μM.

Western Blot Analysis^[2]

Cell Line:

Vascular smooth muscle cells
Concentration:

20 μM
Incubation Time:

2 hours
Result:

Increased of 1.8-fold in the conversion of pro-caspase-3 into active form, and showed better effect with 20 ng/ml PDGF-BB adding.

Apoptosis Analysis^[2]

Cell Line:

Vascular smooth muscle cells
Concentration:

20 μM
Incubation Time:

6 hours
Result:

Induced vascular smooth muscle cells apoptosis by 3-fold, and exhibited 5-fold apoptosis with 20 ng/ml PDGF-BB adding.

Cell Viability Assay^[2]

Cell Line:

Vascular smooth muscle cells
Concentration:

20 μM
Incubation Time:

30 min
Result:

Increased PP2A activity 1.7-flod and increased ROS production 2-fold in vascular smooth muscle cells.

In Vivo

N-Ethylmaleimide (10 mg/kg; i.h.) promotes the prevalence situation of mice with acute gastric ulcers^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Male Wistar rats with acute gastric ulcers induced by absolute ethanol injection^[3]
Dosage:

10 mg/kg
Administration:

Subcutaneous injection; 10 mg/kg once
Result:

Increased the lesion area of acute gastric ulcers and attenuated the gastroprotective effect of PAG in rats.

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

CAS No. 128-53-0
Appearance <43°C Solid,>46°C Liquid
Molecular Weight 125.13
Formula C₆H₇NO₂
Color White to light yellow
SMILES

O=C(C=C1)N(CC)C1=O
Synonyms

NEM
Initial Source
- Microorganisms
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (44)

All Cell Proliferation/Viability AssayFlow CytometryIn Vivo Efficacy StudyIHC

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Journal Impact Factor

J Hematol Oncol

METTL16-SENP3-LTF axis confers ferroptosis resistance and facilitates tumorigenesis in hepatocellular carcinoma. [Abstract]

2024 Sep 2;17(1):78. PMID: 39218945
Cancer Res

tRF-21LeuTAA Promotes Oxidative Stress by Altering Glutathione Metabolic Enzymes to Support Prostate Cancer Progression. [Abstract]

2025 Aug 29. PMID: 40882020
Autophagy

S-palmitoylation coordinates the trafficking of ATG9A to mediate autophagy initiation. [Abstract]

2025 May 31:1-21. PMID: 40394978
Nat Commun

Myocardial mitochondrial antiviral signaling protein promotes heart Ischemia-reperfusion injury via RIG-I signaling in mice. [Abstract]

2025 Jun 2;16(1):5101. PMID: 40456736
Theranostics

GPAT3 is a potential therapeutic target to overcome sorafenib resistance in hepatocellular carcinoma. [Abstract]

2024 Jun 1;14(9):3470-3485. PMID: 38948063

N-Ethylmaleimide purchased from MedChemExpress. Usage Cited in: Theranostics. 2024 Jun 1;14(9):3470-3485. [Abstract]

MHCC97H SR and Hep3B SR cells were treated with sorafenib for 24 hours, with or without NEM (10 μM) or FSG67 (30 μM), and cell death was assessed by the CCK-8 assay.

N-Ethylmaleimide purchased from MedChemExpress. Usage Cited in: Theranostics. 2024 Jun 1;14(9):3470-3485. [Abstract]

MHCC97H SR and Hep3B SR cells were treated with sorafenib for 24 hours, with or without NEM (10 μM) or FSG67 (30 μM), and apoptosis was assessed by flow cytometry.

N-Ethylmaleimide purchased from MedChemExpress. Usage Cited in: Theranostics. 2024 Jun 1;14(9):3470-3485. [Abstract]

The sensitivity of MHCC97H SR cells to sorafenib, NEM (1 mg/kg, every 2 days), and FSG67 was evaluated in vivo. The figure shows a solid view of the tumor.

N-Ethylmaleimide purchased from MedChemExpress. Usage Cited in: Theranostics. 2024 Jun 1;14(9):3470-3485. [Abstract]

The sensitivity of MHCC97H SR cells to sorafenib, NEM (1 mg/kg, every 2 days), and FSG67 was evaluated in vivo. The figure shows the tumor growth curve.

N-Ethylmaleimide purchased from MedChemExpress. Usage Cited in: Theranostics. 2024 Jun 1;14(9):3470-3485. [Abstract]

The sensitivity of MHCC97H SR cells to sorafenib, NEM (1 mg/kg, every 2 days), and FSG67 was evaluated in vivo. The figure shows the results of p65 and Oil Red O staining immunohistochemical (IHC) analysis.
J Exp Clin Cancer Res

Protein disulfide-isomerase A4 confers glioblastoma angiogenesis promotion capacity and resistance to anti-angiogenic therapy. [Abstract]

2023 Mar 30;42(1):77. PMID: 36997943
Adv Sci (Weinh)

MCU Upregulation Overactivates Mitophagy by Promoting VDAC1 Dimerization and Ubiquitination in the Hepatotoxicity of Cadmium. [Abstract]

2023 Mar;10(7):e2203869. PMID: 36642847
Sci Adv

ATP13A1 engages SEC61 to facilitate substrate-specific translocation. [Abstract]

2025 Jun 13;11(24):eadt1346. PMID: 40498833
Cell Death Dis

E3 Ubiquitin ligases Cbl-b and c-Cbl maintain the homeostasis of macrophages by regulating the M-CSF/M-CSFR signaling axis. [Abstract]

2025 Oct 7;16(1):716. PMID: 41057360
Appl Phys Rev

2026 Jan 21.
J Hazard Mater

Oral exposure to polystyrene nanoplastics reduced male fertility and even caused male infertility by inducing testicular and sperm toxicities in mice. [Abstract]

2023 Jul 15:454:131470. PMID: 37116333
Cell Death Discov

Trim15 stabilizes VDAC3 via ubiquitination to suppress autophagy and enhance chemosensitivity in hypopharyngeal squamous cell carcinoma. [Abstract]

2026 Jan 30;12(1):88. PMID: 41617671
Cell Death Discov

Quantitative chemical proteomics reveals that phenethyl isothiocyanate covalently targets BID to promote apoptosis. [Abstract]

2024 Oct 29;10(1):456. PMID: 39472556
Cell Death Discov

WWP1 upregulation predicts poor prognosis and promotes tumor progression by regulating ubiquitination of NDFIP1 in intrahepatic cholangiocarcinoma. [Abstract]

2022 Mar 9;8(1):107. PMID: 35264565
Oncogene

ZDHHC9-mediated KLF5 palmitoylation enhances the cAMP/PKA/CREB axis to promote colorectal cancer progression. [Abstract]

2026 Apr;45(15):1370-1385. PMID: 41882103
Oncogene

SENP1-mediated deSUMOylation of YBX1 promotes colorectal cancer development through the SENP1-YBX1-AKT signaling axis. [Abstract]

2025 May;44(19):1361-1374. PMID: 39988696
Cell Chem Biol

Temporal proteomics reveal specific cell cycle oncoprotein downregulation by p97/VCP inhibition. [Abstract]

2022 Mar 17;29(3):517-529.e5. PMID: 34847375
Antioxidants (Basel)

Thioamide Compound H0802 Enhances Hypoxia Tolerance by Mimicking Hypoxia-Adaptive Reprogramming of Glucose and Oxygen Metabolism. [Abstract]

2026 Apr 22;15(5):525. PMID: 42193148
Free Radic Biol Med

Proteome-wide quantitative analysis of redox cysteine in porcine uterus reveals deoxynivalenol induced oxidative stress via protein hyperoxidation. [Abstract]

2026 Jun 27:S0891-5849(26)00902-0. PMID: 42364855
Clin Transl Med

Glutaredoxin-1 promotes lymphangioleiomyomatosis progression through inhibiting Bim-mediated apoptosis via COX2/PGE2/ERK pathway. [Abstract]

2023 Jul;13(7):e1333. PMID: 37478294
J Med Chem

Dual-Functional Anti-SIRPα-cGAMP Conjugate Reprograms the Tumor Immune Microenvironment and Enhances Antitumor Immunity. [Abstract]

2026 Apr 23;69(8):9603-9614. PMID: 41918375
J Med Chem

Modulation of Tumor Microenvironment via Antibody Mediated Precision Delivery of CpG to Myeloid Cell. [Abstract]

2026 Feb 26;69(4):4900-4912. PMID: 41649040
J Agric Food Chem

Discovery of 10-Shogaol from Zingiber officinale with Broad Antiviral and Anti-inflammatory Activity against Dengue and Zika Viruses Using Activity-Guided Molecular Networking. [Abstract]

2026 Jun 24;74(24):18723-18740. PMID: 42270434
Biochem Pharmacol

TRAF6-mediated ubiquitination of AKT1 in the nucleus occurs in a β-arrestin2-dependent manner upon insulin stimulation. [Abstract]

2024 Aug:226:116362. PMID: 38871335
Life Sci

Salvianolic acid A inhibits tumor cell proliferation and induces chromosomal abnormalities by blocking the citrullinating enzyme PADs. [Abstract]

2026 Aug 1:398:124444. PMID: 42107692
Int Immunopharmacol

Cyclosporin A indirectly suppresses NK cells activity in renal microvascular inflammation by inducing ubiquitination-dependent degradation of ULBP1. [Abstract]

2026 Jan 1;168(Pt 2):115929. PMID: 41297348
J Enzyme Inhib Med Chem

Natural product sennoside B disrupts liquid-liquid phase separation of SARS-CoV-2 nucleocapsid protein by inhibiting its RNA-binding activity. [Abstract]

2025 Dec;40(1):2501743. PMID: 40371698
Microchem J

2024 Dec.
Sci Rep

Cysteine-reactive mitigators of small vessel disease-related NOTCH3 mutants. [Abstract]

2026 Mar 20;16(1):14300. PMID: 41862552
Cell Signal

KGF impedes TRIM21-enhanced stabilization of keratin 10 mediating differentiation in hypopharyngeal cancer. [Abstract]

2025 Mar:127:111614. PMID: 39848455
Virol Sin

Classical swine fever virus NS5A protein antagonizes innate immune response by inhibiting the NF-κB signaling. [Abstract]

2023 Dec;38(6):900-910. PMID: 37714433
J Inflamm Res

Neutrophil Extracellular Traps Induce Alveolar Macrophage Pyroptosis by Regulating NLRP3 Deubiquitination, Aggravating the Development of Septic Lung Injury. [Abstract]

2023 Feb 27:16:861-877. PMID: 36876152
FASEB J

UFMylation of HRD1 regulates endoplasmic reticulum homeostasis. [Abstract]

2023 Nov;37(11):e23221. PMID: 37795761
ACS Chem Biol

Targeted O-GlcNAcylation of CK2α Triggers Its Ubiquitin-Proteasome Degradation and Alters Downstream Phosphorylation. [Abstract]

2025 Jul 18;20(7):1646-1659. PMID: 40521680
Anim Cells Syst

Blocking SLC7A11 attenuates the proliferation of esophageal squamous cell carcinoma cells. [Abstract]

2024 May 11;28(1):237-250. PMID: 38741950
Stem Cells Int

FAIM Enhances the Efficacy of Mesenchymal Stem Cell Transplantation by Inhibiting JNK-Induced c-FLIP Ubiquitination and Degradation. [Abstract]

2022 Sep 30:2022:3705637. PMID: 36248256
Cell Biochem Funct

SENP3 Promotes Hepatocyte Steatosis via De-SUMOylation of SREBP2. [Abstract]

2025 Nov;43(11):e70141. PMID: 41254938
Chem Biol Drug Des

Sevoflurane Postconditioning Regulates Mitophagy Through the PINK1/Parkin Pathway to Alleviate Myocardial Microcirculatory Reperfusion Injury. [Abstract]

2025 Dec;106(6):e70228. PMID: 41406175
bioRxiv

Distinct Proteasomal Pathways Drive Oncogenic PPM1D Activation. [Abstract]

2026 Jun 9:2026.06.01.729344. PMID: 42282570
bioRxiv

2026 Apr 23.
bioRxiv

2026 Mar 18.
bioRxiv

2026 Mar 27.
Research Square Preprint

2024 Dec 03.
bioRxiv

2024 Feb 12.

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (799.17 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 50 mg/mL (399.58 mM; Need ultrasonic)

Ethanol : 12.5 mg/mL (99.90 mM; Need ultrasonic)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

7.9917 mL

39.9584 mL

79.9169 mL

5 mM

1.5983 mL

7.9917 mL

15.9834 mL

10 mM

0.7992 mL

3.9958 mL

7.9917 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (16.62 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (16.62 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (16.62 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS

Solubility: 100 mg/mL (799.17 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Working solution concentration: 0.22 mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact

MedChemExpress (MCE) .

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (286 KB)
SDS (642 KB)
- English - EN (642 KB)
- Français - FR (642 KB)
- Deutsch - DE (642 KB)
- Norwegian - NO (642 KB)
- Español - ES (642 KB)
- Swedish - SV (642 KB)
- Italian - IT (642 KB)
- Korean - KR (642 KB)
- Portuguese - PT (642 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Moriyama A, et al. Porcine muscle prolyl endopeptidase and its endogenous substrates. J Biochem. 1988 Jul;104(1):112-7. [Content Brief]

[2]. Yellaturu CR, et al. N-Ethylmaleimide inhibits platelet-derived growth factor BB-stimulated Akt phosphorylation via activation of protein phosphatase 2A. J Biol Chem. 2002 Oct 18;277(42):40148-55. [Content Brief]

[3]. Matsuda H, et al. Roles of capsaicin-sensitive sensory nerves, endogenous nitric oxide, sulfhydryls, and prostaglandins in gastroprotection by momordin Ic, an oleanolic acid oligoglycoside, on ethanol-induced gastric mucosal lesions in rats. Life Sci. 1999;65(2):PL27-32. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
Ethanol / H₂O / DMSO	1 mM	7.9917 mL	39.9584 mL	79.9169 mL	199.7922 mL
	5 mM	1.5983 mL	7.9917 mL	15.9834 mL	39.9584 mL
	10 mM	0.7992 mL	3.9958 mL	7.9917 mL	19.9792 mL
	15 mM	0.5328 mL	2.6639 mL	5.3278 mL	13.3195 mL
	20 mM	0.3996 mL	1.9979 mL	3.9958 mL	9.9896 mL
	25 mM	0.3197 mL	1.5983 mL	3.1967 mL	7.9917 mL
	30 mM	0.2664 mL	1.3319 mL	2.6639 mL	6.6597 mL
	40 mM	0.1998 mL	0.9990 mL	1.9979 mL	4.9948 mL
	50 mM	0.1598 mL	0.7992 mL	1.5983 mL	3.9958 mL
	60 mM	0.1332 mL	0.6660 mL	1.3319 mL	3.3299 mL
	80 mM	0.0999 mL	0.4995 mL	0.9990 mL	2.4974 mL
H₂O / DMSO	100 mM	0.0799 mL	0.3996 mL	0.7992 mL	1.9979 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Keywords

N-Ethylmaleimide

44 Cited Publications

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N-Ethylmaleimide

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Publications Citing Use of MedChemExpress (MCE) N-Ethylmaleimide

All Cathepsin Isoforms

All Deubiquitinase Isoforms

Biological Activity

Chemical Information

Publications (44)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

N-Ethylmaleimide Related Classifications

HY-D0843 Related Classifications