1. Metabolic Enzyme/Protease
  2. Cathepsin
  3. N-Ethylmaleimide

N-Ethylmaleimide (Synonyms: NEM)

Cat. No.: HY-D0843 Purity: 99.67%
Handling Instructions

N-Ethylmaleimide (NEM), a reagent that alkylates free sulfhydryl groups, is a cysteine protease inhibitor. N-ethylmaleimide specific inhibits phosphate transport in mitochondria.

For research use only. We do not sell to patients.

N-Ethylmaleimide Chemical Structure

N-Ethylmaleimide Chemical Structure

CAS No. : 128-53-0

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 55 In-stock
Estimated Time of Arrival: December 31
10 mg USD 50 In-stock
Estimated Time of Arrival: December 31
50 mg   Get quote  
100 mg   Get quote  

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Based on 1 publication(s) in Google Scholar

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Description

N-Ethylmaleimide (NEM), a reagent that alkylates free sulfhydryl groups, is a cysteine protease inhibitor[1]. N-ethylmaleimide specific inhibits phosphate transport in mitochondria[2].

IC50 & Target

Cysteine protease[1]

Clinical Trial
Molecular Weight

125.13

Formula

C₆H₇NO₂

CAS No.

128-53-0

SMILES

O=C(C=C1)N(CC)C1=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

H2O : 50 mg/mL (399.58 mM; Need ultrasonic)

DMSO : 50 mg/mL (399.58 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 7.9917 mL 39.9584 mL 79.9169 mL
5 mM 1.5983 mL 7.9917 mL 15.9834 mL
10 mM 0.7992 mL 3.9958 mL 7.9917 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 0.92 mg/mL (7.35 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 0.92 mg/mL (7.35 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 0.92 mg/mL (7.35 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
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Keywords:

N-EthylmaleimideNEMCathepsinInhibitorinhibitorinhibit

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Product name:
N-Ethylmaleimide
Cat. No.:
HY-D0843
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