N-Ethylmaleimide-d5
Based on 1 Customer Validation
N-Ethylmaleimide-d5 is the deuterium labeled N-Ethylmaleimide. N-Ethylmaleimide (NEM), a reagent that alkylates free sulfhydryl groups, is a cysteine protease inhibitor. N-ethylmaleimide specific inhibits phosphate transport in mitochondria. N-Ethylmaleimide is also a deubiquitinating enzyme inhibitor.
For research use only. We do not sell to patients.
- Purity: 99.84%
- CAS No.: 360768-37-2
- Formula: C6H2D5NO2
- Molecular Weight:130.16
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Stable heavy isotopes of hydrogen, carbon, and other elements have been incorporated into drug molecules, largely as tracers for quantitation during the drug development process. Deuteration has gained attention because of its potential to affect the pharmacokinetic and metabolic profiles of drugs[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
1. This compound can be used as a tracer
2. This compound can be used as an internal standard for quantitative analysis by NMR, GC-MS, or LC-MS.
Chemical Information
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CAS No. 360768-37-2
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Unlabeled Cas 128-53-0
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Appearance Solid
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Molecular Weight 130.16
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Formula C6H2D5NO2
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Color White to off-white
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SMILES
O=C(C=C1)N(C([2H])([2H])C([2H])([2H])[2H])C1=O
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Synonyms
NEM-d5
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (768.29 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 50 mg/mL (384.14 mM; Need ultrasonic)
Ethanol : 12.5 mg/mL (96.04 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (281 KB)
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SDS (642 KB)
- English - EN (642 KB)
- Français - FR (642 KB)
- Deutsch - DE (642 KB)
- Norwegian - NO (642 KB)
- Español - ES (642 KB)
- Swedish - SV (642 KB)
- Italian - IT (642 KB)
- Korean - KR (642 KB)
- Portuguese - PT (642 KB)
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Handling Instructions (2659 KB)
References
[1]. Russak EM, et al. Impact of Deuterium Substitution on the Pharmacokinetics of Pharmaceuticals. Ann Pharmacother. 2019;53(2):211-216. [Content Brief]
[2]. Wu KH, et al. Cys32 and His105 are the critical residues for the calcium-dependent cysteine proteolyticactivity of CvaB, an ATP-binding cassette transporter. J Biol Chem. 2004 Jan 9;279(2):901-9. [Content Brief]
[4]. Choo YS, et al. Detection of protein ubiquitination. J Vis Exp. 2009;(30):1293. Published 2009 Aug 19. doi:10.3791/1293 [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| Ethanol / H2O / DMSO | 1 mM | 7.6829 mL | 38.4143 mL | 76.8285 mL | 192.0713 mL |
| 5 mM | 1.5366 mL | 7.6829 mL | 15.3657 mL | 38.4143 mL | |
| 10 mM | 0.7683 mL | 3.8414 mL | 7.6829 mL | 19.2071 mL | |
| 15 mM | 0.5122 mL | 2.5610 mL | 5.1219 mL | 12.8048 mL | |
| 20 mM | 0.3841 mL | 1.9207 mL | 3.8414 mL | 9.6036 mL | |
| 25 mM | 0.3073 mL | 1.5366 mL | 3.0731 mL | 7.6829 mL | |
| 30 mM | 0.2561 mL | 1.2805 mL | 2.5610 mL | 6.4024 mL | |
| 40 mM | 0.1921 mL | 0.9604 mL | 1.9207 mL | 4.8018 mL | |
| 50 mM | 0.1537 mL | 0.7683 mL | 1.5366 mL | 3.8414 mL | |
| 60 mM | 0.1280 mL | 0.6402 mL | 1.2805 mL | 3.2012 mL | |
| 80 mM | 0.0960 mL | 0.4802 mL | 0.9604 mL | 2.4009 mL | |
| H2O / DMSO | 100 mM | 0.0768 mL | 0.3841 mL | 0.7683 mL | 1.9207 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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