2. Signaling Pathways
  3. Cell Cycle/DNA Damage
  4. Deubiquitinase
  5. USP25 Isoform

USP25

Ubiquitin-Specific Peptidase 25

USP28 is a deubiquitinase (DUB). Three isoforms of USP25 are generated by alternative splicing. Isoform USP25a is found in most adult and fetal tissues. Isoform USP25b is found in all tissues except heart and skeletal muscle. Isoform USP25m is specifically present in the skeletal muscle and heart and is upregulated during myogenesis. Like most USPs, USP25 has three ubiquitin-binding domains (UBDs) at the N terminus: a ubiquitin-associated domain (UBA) and two tandem ubiquitin-interacting motifs (UIMs). However, no clear structural homology among USP family members has been detected in the extended C-terminal region outside of the catalytic domain. Compared to the more common USP25a and USP25b isoforms, USP25m has an added muscle-specific domain. USP25 is not only distributed in the nucleus and cytoplasm but also can be localized to the endoplasmic reticulum (ER), where it negatively regulates ER-associated degradation (ERAD). USP25 as an essential DUB required for pancreatic ductal adenocarcinoma (PDAC) tumor growth and viability.

USP25 Related Products (1):

Cat. No. Product Name Effect Purity
  • HY-184244
    CAS-010
    		Inhibitor
    CAS-010 (CAS-13312-010) is a potent and selective USP28 inhibitor with an IC50 of 2.2 nM against full-length USP28. CAS-010 acts as a ubiquitin-competitive inhibitor and shows higher selectivity over USP25 (IC50 = 51 nM) and other USP family members, disrupting the 53BP1-USP28 interaction and suppressing p53-dependent transcription of CDKN1A (p21), MDM2, and BAX. CAS-010 can be used for studying USP28-mediated DNA damage response and p53 signaling in cancer-related research.