2. Metabolic Enzyme/Protease Anti-infection
  3. Cathepsin Ser/Thr Protease Virus Protease
  4. Leupeptin hemisulfate

Leupeptin hemisulfate 

Cat. No.: HY-18234A Purity: ≥98.0%
COA Handling Instructions

Leupeptin hemisulfate is a broad-spectrum, membrane-permeable protease inhibitor. Leupeptin hemisulfate potently inhibits serine, cysteine and threonine proteases. Leupeptin hemisulfate inhibits Mpro (the main protease of SARS-CoV-2) and also has anti-inflammatory activity.

For research use only. We do not sell to patients.

Leupeptin hemisulfate Chemical Structure

Leupeptin hemisulfate Chemical Structure

CAS No. : 103476-89-7

Size Price Stock Quantity
Solution
10 mM * 1 mL in Water USD 66 In-stock
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
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Solid
5 mg USD 60 In-stock
10 mg USD 84 In-stock
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Based on 24 publication(s) in Google Scholar

Other Forms of Leupeptin hemisulfate:

Leupeptin hemisulfate Related Antibodies

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Description

Leupeptin hemisulfate is a broad-spectrum, membrane-permeable protease inhibitor. Leupeptin hemisulfate potently inhibits serine, cysteine and threonine proteases. Leupeptin hemisulfate inhibits Mpro (the main protease of SARS-CoV-2) and also has anti-inflammatory activity[1][2][3].

IC50 & Target

Cathepsin B, Cathepsin H, Cathepsin L, Ser/Thr Protease, Mpro[1][2][3].
In Vitro

Leupeptin hemisulfate (0.06-200 µM; 72 h) significantly decreases copy numbers of SARS-CoV-2 viral RNA (vRNA) in Vero cells[1]
Leupeptin hemisulfate inhibits RNA levels of SARS-CoV-2 in Vero cells, with an EC50 value of 42.34 µM[1].
Leupeptin hemisulfate has some inhibitory activity against Mpro, with an IC50 value of 127.2 µM[1].
Leupeptin hemisulfate againsts human coronavirus strain 229E with an IC50 value of 0.4 µg/mL (about 1 µM)[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Leupeptin hemisulfate Related Antibodies

RT-PCR[1]

Cell Line: Vero cells
Concentration: 0.06-200 µM
Incubation Time: 72 h
Result: Significantly decreased copy numbers of SARS-CoV-2 viral RNA (vRNA).
In Vivo

Leupeptin hemisulfate (0, 9, 18, 36 mg/kg; i.p.; single) is well tolerated by the animals and produces a strong, dose-dependent increase in LC3b-II in both the total tissue extracts and the lysosome and autophagosome-enriched pellet fraction[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: C57BL/6NCrl male mice (6-8 weeks old, 20-25 g)[1]
Dosage: 0, 9, 18, 36 mg/kg
Administration: Intraperitoneal injection; single
Result: Promoted the accumulation of LC3b-II in mouse liver.
Molecular Weight

475.59

Appearance

Solid

Formula

C20H38N6O4.1/2H2SO4
CAS No.
Sequence Shortening

Ac-LLR-CHO
SMILES

O=C[C@@H](NC([C@@H](NC([C@@H](NC(C)=O)CC(C)C)=O)CC(C)C)=O)CCCNC(N)=N.[0.5H2SO4]
Structure Classification
Initial Source

Microbial Metabolite
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
In Vitro: 

H2O : 83.33 mg/mL (175.21 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.1027 mL 10.5133 mL 21.0265 mL
5 mM 0.4205 mL 2.1027 mL 4.2053 mL
10 mM 0.2103 mL 1.0513 mL 2.1027 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  PBS

    Solubility: 100 mg/mL (210.27 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

*All of the co-solvents are available by MCE.
Purity & Documentation

Purity: ≥98.0%

COA (250 KB) HNMR (273 KB) MS (72 KB)

Handling Instructions (2659 KB)
References
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Leupeptin hemisulfate Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The dilution calculator equation

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This equation is commonly abbreviated as: C1V1 = C2V2

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× = ×
C1   V1   C2   V2

Keywords:

Leupeptin hemisulfate103476-89-7CathepsinSer/Thr ProteaseVirus ProteaseSerine proteasesSerine endopeptidasesThreonine proteasesMproSARS-CoV-2Vero cellscathepsinserine proteasesthreonine proteasescysteine proteasesInhibitorinhibitorinhibit

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