1-Benzylimidazole attenuates the stemness of breast cancer cells through partially targeting CYP4Z1

Cytochrome P450 (CYP) 4Z1 (CYP4Z1) has recently garnered much interest as its expression predicts a poor prognosis and as a oncogene in breast Cancer, and overexpressed in Other many cancers. We previously showed that CYP4Z1 acts as a promoter of Cancer Stem Cells (CSCs) to facilitate the occurrence and development of breast Cancer. Here, RNA Sequencing found that 1-benzylimidazole (1-Benzy) held a preferable correlation with breast Cancer and suppressed the expression of CSC makers. Further functional experiments, including mammary spheroid formation, wound-healing, transwell-invasion, detection of tumor initiation, and metastatic ability, showed that 1-Benzy suppressed the stemness and metastasis of breast Cancer cells. Additionally, we further demonstrated that CYP4Z1 is necessary for 1-Benzy-mediated suppression on breast Cancer stemness and 1-Benzy exerted a weaker effect in breast Cancer cells with CYP4Z1 knockdown. Taken together, our data suggest that 1-Benzy might be a potential drug suppressing breast Cancer stemness via targeting CYP4Z1.