2. Metabolic Enzyme/Protease Anti-infection
  3. Ser/Thr Protease Virus Protease Cathepsin
  4. Leupeptin

Leupeptin is a broad-spectrum, membrane-permeable protease inhibitor. Leupeptin potently inhibits serine, cysteine and threonine proteases. Leupeptin inhibits Mpro (the main protease of SARS-CoV-2) and also has anti-inflammatory activity.

For research use only. We do not sell to patients.

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CAS No. : 55123-66-5

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Leupeptin Related Antibodies

Top Publications Citing Use of Products

61 Publications Citing Use of MCE Leupeptin

WB

    Leupeptin purchased from MedChemExpress. Usage Cited in: J Hematol Oncol. 2024 Sep 2;17(1):78.  [Abstract]

    The LTF expression was detected under MG132 (10 μM) or Leupeptin (100 μM) treatment.

    Leupeptin purchased from MedChemExpress. Usage Cited in: Autophagy. 2025 Feb;21(2):352-373.  [Abstract]

    Immunoblotting showing the level of PLCG1 in NPC after treatment with Leu (10 μM), NH4Cl (20 mM), or their combination for 12 h. Right panels represent normalized (/NC) band intensities of interest.

    Leupeptin purchased from MedChemExpress. Usage Cited in: Autophagy. 2025 Feb;21(2):352-373.  [Abstract]

    Western blot showing PLCG1 levels in NPC treated with a combination of Leu (10 μM) and NH4Cl (N/L) for 3, 6, and 9 h. Right panels represent normalized (/NC) band intensities of interest.

    Leupeptin purchased from MedChemExpress. Usage Cited in: Cell Death Differ. 2023 Jan;30(1):137-151.  [Abstract]

    Western blot analysis of SLC7A11, SOCS2 and tubulin proteins in SK-Hep-1 and HepG2 cells at 4 h after 4 Gy IR. Leupeptin (50 μM) were added before IR.

    Leupeptin purchased from MedChemExpress. Usage Cited in: Autophagy. 2021 Aug;17(8):1873-1888.  [Abstract]

    661 W cells were transfected with the indicated siRNA for 24 h. Then, 661 W cells were treated with 10 μg/ml Leupeptin for 24 h. Cell lysates were collected for western blot analysis of LC3B and GAPDH.

    View All Cathepsin Isoform Specific Products:

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    Cathepsin B Cathepsin C Cathepsin D Cathepsin E Cathepsin K Cathepsin L Cathepsin S

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    Description

    Leupeptin is a broad-spectrum, membrane-permeable protease inhibitor. Leupeptin potently inhibits serine, cysteine and threonine proteases. Leupeptin inhibits Mpro (the main protease of SARS-CoV-2) and also has anti-inflammatory activity[1][2][3].

    IC50 & Target

    Cathepsin B, Cathepsin H, Cathepsin L, Ser/Thr Protease, Mpro[1][2][3].
    Cellular Effect
    Cell Line Type Value Description References
    HeLa IC50
    > 224 μM
    Compound: 1
    Antiviral activity against human rhinovirus-14 in HeLa cells
    Antiviral activity against human rhinovirus-14 in HeLa cells
    		10.1016/S0960-894X(96)00537-9
    In Vitro

    Leupeptin (0.06-200 μM; 72 h) significantly decreases copy numbers of SARS-CoV-2 viral RNA (vRNA) in Vero cells[1]
    Leupeptin inhibits RNA levels of SARS-CoV-2 in Vero cells, with an EC50 value of 42.34 μM[1].
    Leupeptin has some inhibitory activity against Mpro, with an IC50 value of 127.2 μM[1].
    Leupeptin againsts human coronavirus strain 229E with an IC50 value of 0.4 μg/mL (about 1 μM)[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Leupeptin Related Antibodies

    RT-PCR[1]

    Cell Line: Vero cells
    Concentration: 0.06-200 µM
    Incubation Time: 72 h
    Result: Significantly decreased copy numbers of SARS-CoV-2 viral RNA (vRNA).
    In Vivo

    Leupeptin (0, 9, 18, 36 mg/kg; i.p.; single) is well tolerated by the animals and produces a strong, dose-dependent increase in LC3b-II in both the total tissue extracts and the lysosome and autophagosome-enriched pellet fraction[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: C57BL/6NCrl male mice (6-8 weeks old, 20-25 g)[1].
    Dosage: 0, 9, 18, 36 mg/kg
    Administration: Intraperitoneal injection; single
    Result: Promoted the accumulation of LC3b-II in mouse liver.
    Molecular Weight

    426.55

    Formula

    C20H38N6O4
    CAS No.
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    Please store the product under the recommended conditions in the Certificate of Analysis.
    Purity & Documentation
    References
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    Leupeptin Related Classifications

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    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Leupeptin55123-66-5Ser/Thr ProteaseVirus ProteaseCathepsinSerine proteasesSerine endopeptidasesThreonine proteasesMproSARS-CoV-2Vero cellscathepsinserine proteasesthreonine proteasescysteine proteasesInhibitorinhibitorinhibit

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