Thrombin (MW 37kDa)

Cat. No.: HY-114164: Data Sheet
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Thrombin (MW 37kDa) is a Na⁺-activated, allosteric serine protease that plays opposing functional roles in blood coagulation. Thrombin recognition sequence and can be used to digest GST-tagged proteins.

For research use only. We do not sell to patients.

CAS No. : 9002-04-4

Size	Price	Stock	Quantity
1000 U		In-stock	Estimated Time of Arrival: December 31
2000 U		In-stock	Estimated Time of Arrival: December 31

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Based on 7 publication(s) in Google Scholar

Other Forms of Thrombin (MW 37kDa):

7 Publications Citing Use of MCE Thrombin (MW 37kDa)

•bioRxiv. 2026 Mar 5:2026.03.03.709348.

Flow Cytometry

Cell Imaging/Staining

ELISA

Cell Migration/Invasion Assay

: Thrombin (MW 37kDa) purchased from MedChemExpress. Usage Cited in: Cancer Commun (Lond). 2025 Feb;45(2):143-166. [Abstract]; Platelets were treated with Fc fragment, Thrombin (1 µg/mL; 2 h), EphB1‐Fc, Thrombin + EphB1‐Fc, mono EphB1, mono EphB1 + EphB1‐Fc, or EFNB1 Ab + EphB1‐Fc. Platelets without treatment served as controls. CD62P expression in activated platelets was tested by flow cytometry analysis. Representative flow cytometry analysis of CD62P expression is presented on the left. The right graph shows the percentage of CD62P+ platelets. Data were presented as the means ± SD, with n = 3 per group.

: Thrombin (MW 37kDa) purchased from MedChemExpress. Usage Cited in: Mol Carcinog. 2024 Jul;63(7):1288-1302. [Abstract]; Effect of knockdown of KDM4E and combined overexpression of BICD1 on the proliferative activity of Thrombin (1 pM)-activated TNBC cells detected by EdU staining (scale bar: 50 μm).

: Thrombin (MW 37kDa) purchased from MedChemExpress. Usage Cited in: Mol Carcinog. 2024 Jul;63(7):1288-1302. [Abstract]; Effect of knockdown of KDM4E and combined overexpression of BICD1 on the invasive activity of Thrombin (1 pM)-activated TNBC cells detected by Transwell assay (scale bar: 50 μm). *p < 0.05 versus the sh-scramble group, #p < 0.05 versus the sh-KDM4E + oe-NC group, &p < 0.05 versus the blank group.

: Thrombin (MW 37kDa) purchased from MedChemExpress. Usage Cited in: Allergy. 2022 Jul;77(7):2104-2120. [Abstract]; Western blots for the HA tag on RRL-expressed IL-33 showing the Thrombin cleavage of human pro-IL-33 into the two main forms, ~18 and ~25 kDa, and the inhibition of these cleavage events by the Thrombin inhibitor Argatroban (50 μg/ml). Cleavage occurred in a time-dependent manner (15 min, 30 min, 1 h, 2 h and 4 h; 0.075 U Thrombin; 15 μL buffer) (A) and a dose-dependent manner (0.01875, 0.0375, 0.075, 0.15 and 0.3 U; RT for 1 h; 15 μL buffer) (B).

: Thrombin (MW 37kDa) purchased from MedChemExpress. Usage Cited in: Allergy. 2022 Jul;77(7):2104-2120. [Abstract]; L-33-dependent IL-5, IL-13 and IL-4 production by ST2+ CD4 cells incubated with or without Thrombin (50 pg RRL; 0.075 U Thrombin; RT for 1 h) and with or without a neutralizing hIL-33 mAb. The bars and error bars represent the mean ± SEM; *p < .05; **p < .01; ***p < .001.

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Description	Thrombin (MW 37kDa) is a Na⁺-activated, allosteric serine protease that plays opposing functional roles in blood coagulation. Thrombin recognition sequence and can be used to digest GST-tagged proteins.
In Vitro	p62 is cleaved from the agarose beads as well as the GST tag by addition of Thrombin (1 Unit/100 μg protein) at room temperature for 20 h^[3]. Thrombin solution: Dissolve in PBS prechilled to 4°C. Swirl gently. Store solution in small aliquots at -80°C to preserve activity. Cleavage buffer: PBS (140 mM NaCl, 2.7 mM KCl, 10 mM Na₂HPO₄, 1.8 mM KH₂PO₄) pH 7.3. Elution buffer: 50 mM Tris-HCl, 10 mM reduced glutathione, pH 8.0 MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Thrombin (MW 37kDa) Related Antibodies
Molecular Weight	37000(Average)
CAS No.	9002-04-4
Appearance	Solid
Color	White to off-white
SMILES	[Thrombin (MW 37kDa)]
Shipping	Room temperature in continental US; may vary elsewhere.
Storage	Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation	Select Batch: Data Sheet (269 KB) SDS (419 KB) COA (242 KB) Handling Instructions (2659 KB)
References	[1]. Golderman V, et al. Thrombin and the Protease-Activated Receptor-1 in Organophosphate-Induced Status Epilepticus. J Mol Neurosci. 2018 Dec 4. [Content Brief] [2]. Krenzlin H, et al. The Importance of Thrombin in Cerebral Injury and Disease. Int J Mol Sci. 2016 Jan 11;17(1). [Content Brief] [3]. Geetha T, et al. p62 serves as a shuttling factor for TrkA interaction with the proteasome. Biochem Biophys Res Commun. 2008 Sep 12;374(1):33-7. [Content Brief]