1. Metabolic Enzyme/Protease
  2. Thrombin
  3. Thrombin (MW 37kDa)

Thrombin (MW 37kDa) 

Cat. No.: HY-114164
Handling Instructions

Thrombin (MW 37kDa) is a Na+-activated, allosteric serine protease that plays opposing functional roles in blood coagulation. Thrombin recognition sequence and can be used to digest GST-tagged proteins.

For research use only. We do not sell to patients.

Thrombin  (MW 37kDa) Chemical Structure

Thrombin (MW 37kDa) Chemical Structure

CAS No. : 9002-04-4

Size Price Stock Quantity
1000 U USD 170 In-stock
Estimated Time of Arrival: December 31
2000 U USD 280 In-stock
Estimated Time of Arrival: December 31

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Based on 3 publication(s) in Google Scholar

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Description

Thrombin (MW 37kDa) is a Na+-activated, allosteric serine protease that plays opposing functional roles in blood coagulation. Thrombin recognition sequence and can be used to digest GST-tagged proteins.

In Vitro

One unit of enzyme cleaves > 90% of 100 µg of a test GST-tagged protein when incubated in 1× PBS at 22°C for 16 h.
Thrombin solution: Dissolve 500 units in 0.5 mL of PBS prechilled to 4°C. Swirl gently. Store solution in small aliquots at -80°C to preserve activity.
Cleavage buffer: PBS (140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4) pH 7.3.
Elution buffer: 50 mM Tris-HCl, 10 mM reduced glutathione, pH 8.0

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

37000(Average)

CAS No.
SMILES

[Thrombin]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Purity & Documentation

References
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Product Name:
Thrombin (MW 37kDa)
Cat. No.:
HY-114164
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