Rat Thrombin
Based on 8 publication(s) in Google Scholar
Rat Thrombin is a Na+-activated, allosteric serine protease that plays opposing functional roles in blood coagulation. Thrombin recognition sequence and can be used to digest GST-tagged proteins.
For research use only. We do not sell to patients.
- CAS No.: 9002-04-4
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Rat Thrombin
More- Cancer Commun (Lond). 2025 Feb;45(2):143-166. [Abstract]
- Allergy. 2022 Jul;77(7):2104-2120. [Abstract]
- Stem Cell Res Ther. 2020 Feb 21;11(1):76. [Abstract]
- Front Pharmacol. 2022 Jan 10;12:792263. [Abstract]
- Antiviral Res. 2023 Jun:214:105606. [Abstract]
- Mol Carcinog. 2024 Jul;63(7):1288-1302. [Abstract]
- University of Utah. 2026.
- bioRxiv. 2026 Mar 5:2026.03.03.709348. [Abstract]
Customer Validation & Images
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Flow Cytometry
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Cell Imaging/Staining
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Cell Migration/Invasion Assay
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WB
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ELISA
Biological Activity
p62 is cleaved from the agarose beads as well as the GST tag by addition of Rat Thrombin (1 Unit/100 μg protein) at room temperature for 20 h[3].
Rat Thrombin solution: Dissolve in PBS prechilled to 4°C. Swirl gently. Store solution in small aliquots at -80°C to preserve activity.
Cleavage buffer: PBS (140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4) pH 7.3.
Elution buffer: 50 mM Tris-HCl, 10 mM reduced glutathione, pH 8.0
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 9002-04-4
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Appearance Solid
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Color White to off-white
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SMILES
[Rat Thrombin]
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Shipping
Shipping with dry ice.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications (8)
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Journal Impact Factor
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Most Recent
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Cancer Commun (Lond)
Reciprocal tumor-platelet interaction through the EPHB1-EFNB1 axis in the liver metastatic niche promotes metastatic tumor outgrowth in pancreatic ductal adenocarcinoma. [Abstract]2025 Feb;45(2):143-166. PMID: 39648610
Rat Thrombin purchased from MedChemExpress. Usage Cited in: Cancer Commun (Lond). 2025 Feb;45(2):143-166. [Abstract]
Platelets were treated with Fc fragment, Thrombin (1 µg/mL; 2 h), EphB1‐Fc, Thrombin + EphB1‐Fc, mono EphB1, mono EphB1 + EphB1‐Fc, or EFNB1 Ab + EphB1‐Fc. Platelets without treatment served as controls. CD62P expression in activated platelets was tested by flow cytometry analysis. Representative flow cytometry analysis of CD62P expression is presented on the left. The right graph shows the percentage of CD62P+ platelets. Data were presented as the means ± SD, with n = 3 per group.
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Allergy
2022 Jul;77(7):2104-2120. PMID: 34995358
Rat Thrombin purchased from MedChemExpress. Usage Cited in: Allergy. 2022 Jul;77(7):2104-2120. [Abstract]
Western blots for the HA tag on RRL-expressed IL-33 showing the Thrombin cleavage of human pro-IL-33 into the two main forms, ~18 and ~25 kDa, and the inhibition of these cleavage events by the Thrombin inhibitor Argatroban (50 μg/ml). Cleavage occurred in a time-dependent manner (15 min, 30 min, 1 h, 2 h and 4 h; 0.075 U Thrombin; 15 μL buffer) (A) and a dose-dependent manner (0.01875, 0.0375, 0.075, 0.15 and 0.3 U; RT for 1 h; 15 μL buffer) (B).
Rat Thrombin purchased from MedChemExpress. Usage Cited in: Allergy. 2022 Jul;77(7):2104-2120. [Abstract]
L-33-dependent IL-5, IL-13 and IL-4 production by ST2+ CD4 cells incubated with or without Thrombin (50 pg RRL; 0.075 U Thrombin; RT for 1 h) and with or without a neutralizing hIL-33 mAb. The bars and error bars represent the mean ± SEM; *p < .05; **p < .01; ***p < .001.
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Stem Cell Res Ther
Effects of SW033291 on the myogenesis of muscle-derived stem cells and muscle regeneration. [Abstract]2020 Feb 21;11(1):76. PMID: 32085799 -
Front Pharmacol
Hsp47 Inhibitor Col003 Attenuates Collagen-Induced Platelet Activation and Cerebral Ischemic-Reperfusion Injury in Rats. [Abstract]2022 Jan 10;12:792263. PMID: 35082674 -
Antiviral Res
Omicsynin B4 potently blocks coronavirus infection by inhibiting host proteases cathepsin L and TMPRSS2. [Abstract]2023 Jun:214:105606. PMID: 37076089 -
Mol Carcinog
Baicalein promotes KDM4E to induce BICD1 and inhibit triple-negative breast cancer progression by blocking PAR1 signaling. [Abstract]2024 Jul;63(7):1288-1302. PMID: 38607237
Rat Thrombin purchased from MedChemExpress. Usage Cited in: Mol Carcinog. 2024 Jul;63(7):1288-1302. [Abstract]
Effect of knockdown of KDM4E and combined overexpression of BICD1 on the proliferative activity of Thrombin (1 pM)-activated TNBC cells detected by EdU staining (scale bar: 50 μm).
Rat Thrombin purchased from MedChemExpress. Usage Cited in: Mol Carcinog. 2024 Jul;63(7):1288-1302. [Abstract]
Effect of knockdown of KDM4E and combined overexpression of BICD1 on the invasive activity of Thrombin (1 pM)-activated TNBC cells detected by Transwell assay (scale bar: 50 μm). *p < 0.05 versus the sh-scramble group, #p < 0.05 versus the sh-KDM4E + oe-NC group, &p < 0.05 versus the blank group.
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bioRxiv
Protease-Activated Receptor 1 as an Endogenous Model of Peptidergic Gαq-Gα12-Biased G Protein Signaling. [Abstract]2026 Mar 5:2026.03.03.709348. PMID: 41847045
Purity & Documentation
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Data Sheet (263 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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