Canine Thrombin
Based on 8 publication(s) in Google Scholar
Canine Thrombin is a Canine serine protease that plays a central role in blood coagulation. Canine Thrombin stimulates macrophages to polarize into a unique phenotype characterized by anti-inflammatory and pro-repair properties. Canine Thrombin activates PAR1, induces the production of MCP-1, MMP3 and VEGF in intervertebral discs, and causes degradation of the cartilage matrix and destruction of intervertebral disc structure. Canine Thrombin activity increases significantly in paraoxon-induced status epilepticus.
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- CAS No.: 9002-04-4
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Biological Activity
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Publications Citing Use of MedChemExpress (MCE) Canine Thrombin
More- Cancer Commun (Lond). 2025 Feb;45(2):143-166. [Abstract]
- Allergy. 2022 Jul;77(7):2104-2120. [Abstract]
- Stem Cell Res Ther. 2020 Feb 21;11(1):76. [Abstract]
- Front Pharmacol. 2022 Jan 10;12:792263. [Abstract]
- Antiviral Res. 2023 Jun:214:105606. [Abstract]
- Mol Carcinog. 2024 Jul;63(7):1288-1302. [Abstract]
- University of Utah. 2026.
- bioRxiv. 2026 Mar 5:2026.03.03.709348. [Abstract]
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Flow Cytometry
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Cell Imaging/Staining
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Cell Migration/Invasion Assay
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WB
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ELISA
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Biological Activity
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PAR1 |
MMP-3 |
MMP-9 |
Canine Thrombin (100 nM; 72 h) induces degeneration in cultured intervertebral discs and promotes the production of MCP-1 and VEGF[1].
Canine Thrombin (100 nM) induces the polarization of macrophages toward an M1-like phenotype by upregulating the expression of M1 markers and downregulating the expression of M2 markers[1].
Canine Thrombin (0.1-1 U/mL; 12-48 h) upregulates the expressions of CCL22, MRC-1 (CD206), MMP9 and CD36 in macrophages[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 9002-04-4
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SMILES
[Canine Thrombin]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications (8)
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Journal Impact Factor
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Most Recent
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Cancer Commun (Lond)
Reciprocal tumor-platelet interaction through the EPHB1-EFNB1 axis in the liver metastatic niche promotes metastatic tumor outgrowth in pancreatic ductal adenocarcinoma. [Abstract]2025 Feb;45(2):143-166. PMID: 39648610
Canine Thrombin purchased from MedChemExpress. Usage Cited in: Cancer Commun (Lond). 2025 Feb;45(2):143-166. [Abstract]
Platelets were treated with Fc fragment, Thrombin (1 µg/mL; 2 h), EphB1‐Fc, Thrombin + EphB1‐Fc, mono EphB1, mono EphB1 + EphB1‐Fc, or EFNB1 Ab + EphB1‐Fc. Platelets without treatment served as controls. CD62P expression in activated platelets was tested by flow cytometry analysis. Representative flow cytometry analysis of CD62P expression is presented on the left. The right graph shows the percentage of CD62P+ platelets. Data were presented as the means ± SD, with n = 3 per group.
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Allergy
2022 Jul;77(7):2104-2120. PMID: 34995358
Canine Thrombin purchased from MedChemExpress. Usage Cited in: Allergy. 2022 Jul;77(7):2104-2120. [Abstract]
Western blots for the HA tag on RRL-expressed IL-33 showing the Thrombin cleavage of human pro-IL-33 into the two main forms, ~18 and ~25 kDa, and the inhibition of these cleavage events by the Thrombin inhibitor Argatroban (50 μg/ml). Cleavage occurred in a time-dependent manner (15 min, 30 min, 1 h, 2 h and 4 h; 0.075 U Thrombin; 15 μL buffer) (A) and a dose-dependent manner (0.01875, 0.0375, 0.075, 0.15 and 0.3 U; RT for 1 h; 15 μL buffer) (B).
Canine Thrombin purchased from MedChemExpress. Usage Cited in: Allergy. 2022 Jul;77(7):2104-2120. [Abstract]
L-33-dependent IL-5, IL-13 and IL-4 production by ST2+ CD4 cells incubated with or without Thrombin (50 pg RRL; 0.075 U Thrombin; RT for 1 h) and with or without a neutralizing hIL-33 mAb. The bars and error bars represent the mean ± SEM; *p < .05; **p < .01; ***p < .001.
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Stem Cell Res Ther
Effects of SW033291 on the myogenesis of muscle-derived stem cells and muscle regeneration. [Abstract]2020 Feb 21;11(1):76. PMID: 32085799 -
Front Pharmacol
Hsp47 Inhibitor Col003 Attenuates Collagen-Induced Platelet Activation and Cerebral Ischemic-Reperfusion Injury in Rats. [Abstract]2022 Jan 10;12:792263. PMID: 35082674 -
Antiviral Res
Omicsynin B4 potently blocks coronavirus infection by inhibiting host proteases cathepsin L and TMPRSS2. [Abstract]2023 Jun:214:105606. PMID: 37076089 -
Mol Carcinog
Baicalein promotes KDM4E to induce BICD1 and inhibit triple-negative breast cancer progression by blocking PAR1 signaling. [Abstract]2024 Jul;63(7):1288-1302. PMID: 38607237
Canine Thrombin purchased from MedChemExpress. Usage Cited in: Mol Carcinog. 2024 Jul;63(7):1288-1302. [Abstract]
Effect of knockdown of KDM4E and combined overexpression of BICD1 on the proliferative activity of Thrombin (1 pM)-activated TNBC cells detected by EdU staining (scale bar: 50 μm).
Canine Thrombin purchased from MedChemExpress. Usage Cited in: Mol Carcinog. 2024 Jul;63(7):1288-1302. [Abstract]
Effect of knockdown of KDM4E and combined overexpression of BICD1 on the invasive activity of Thrombin (1 pM)-activated TNBC cells detected by Transwell assay (scale bar: 50 μm). *p < 0.05 versus the sh-scramble group, #p < 0.05 versus the sh-KDM4E + oe-NC group, &p < 0.05 versus the blank group.
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bioRxiv
Protease-Activated Receptor 1 as an Endogenous Model of Peptidergic Gαq-Gα12-Biased G Protein Signaling. [Abstract]2026 Mar 5:2026.03.03.709348. PMID: 41847045
Purity & Documentation
References
[1]. Tatsuno R, et al. Thrombin induces degradation of murine intervertebral discs via angiogenesis and M1-like polarization of macrophages. Sci Rep. 2025;15(1):34817. Published 2025 Oct 6. [Content Brief]
[2]. Golderman V, et al. Thrombin and the Protease-Activated Receptor-1 in Organophosphate-Induced Status Epilepticus. J Mol Neurosci. 2018 Dec 4. [Content Brief]
[3]. Ukan Ü, et al. Effect of Thrombin on the Metabolism and Function of Murine Macrophages. Cells. 2022;11(10):1718. Published 2022 May 23. [Content Brief]
[4]. Marino F, et al. Structural basis of Na+ activation mimicry in murine thrombin. J Biol Chem. 2007;282(22):16355-16361. [Content Brief]
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